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EN
The combining ability effects of five silkworm (Bombyx mori L.) strains and their 20 F₁'s including reciprocals were analysed in a 5x5 diallel cross for seven silk yield attributes, viz. effective rate of rearing (ERR), a measure of survival, cocoon yield, cocoon weight, shell weight, raw silk percentage, silk filament length/cocoon and silk reelability. Higher values of specific combining ability (SCA) than the corresponding general combining ability (GCA) for all the attributes except ERR and cocoon yield are indicative of non-additive gene action. The parental strain JC2P was the best general combiner for all the attributes, except cocoon yield. The highest general combining ability effect for cocoon yield was shown by N4. The highest desirable or positive SCA effects resulted from N4 × SH2 (all the attributes except shell weight), JC2P × 14M (all the attributes except cocoon weight and shell weight), 14M × SH2 (all the attributes except ERR), N4 × SPJ1 (all the attributes except shell weight and raw silk percentage). Only one reciprocal, namely SPJ1 × N4, showed positive effects for all the attributes except silk reelability. The improvement of cocoon yield through mass selection followed by intermating and the use of the parental strain JC2P in future cross breeding are discussed.
EN
Bombyx mori. L. (CSR2XCSR4) is a bivolitne crossbreed that produces high quantity of silk. The weights of worms and glands are directly related to the yield of silk, higher larval weights leads to higher silk production. In the present study, quantitative parameters of 5th instar and pupal stages of silkworm larvae were observed. The analysis of quantitative estimation showed that the maximum weights and lengths of silk gland and silk worm were recorded on 7th day of 5th instar larval stage. The day dependent variation in protein concentration was observed in total silk gland 5th instar larval and pupal stages.
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88%
EN
Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
EN
Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.
EN
The article presents the history of the development of sericulture in the world, including Poland. The advantages of natural silk which cause interest in its production and processing in many countries of the world are indicated. A brief description of mulberry silkworm breeding and the technology of silk processing into textile products are presented. The article provides information on the production of natural silk in several countries in 2015-2019. The share of silk in the world global production of fibres is about 0.2%. Over the last few years, the largest amounts of natural silk have been produced by the following countries: China, India and Uzbekistan – the total share of these countries in the world silk production is about 98%.
PL
W artykule przedstawiono historię rozwoju jedwabnictwa w świecie, w tym również w Polsce. Wskazano na walory jedwabiu naturalnego, które powodują zainteresowanie jego produkcją i przetwarzaniem w wielu krajach świata. Przedstawiono także hodowlę jedwabnika morwowego oraz technologię przetwarzania jedwabiu na wyroby. W artykule zawarto dane statystyczne produkcji jedwabiu naturalnego dziewiętnastu krajów w ciągu ostatnich pięciu lat (2015-2019). Udział w produkcji jedwabiu na rynku tekstylnym wynosi około 0,2%. Główne kraje produkujące jedwab naturalny to: Chiny (63%), Indie (32,9%) i Uzbekistan (1,6%) – łączny udział tych krajów w światowej produkcji wynosi 97,5%.
EN
Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
EN
In this study, we report the cloning and characteristics of an adiponectin-like receptor gene from Bombyx mori (BmAdipoR) with highly conserved deduced amino-acid sequences and similar structure to the human adiponectin receptor (AdipoR). Structural analysis of the translated cDNA suggested it encoded a membrane protein with seven transmembrane domains. BmAdipoR was found to be expressed in multiple tissues and highly expressed in Malpighian tubules, fat body and testis. BmNPV (Bombyx morinucleopolyhedrovirus) bacmid system combined with confocal microscopy revealed that BmAdipoR was targeted to the cell membrane. We also found that infection with BmNPV did not have an effect on BmAdipoR mRNA quantity in the midgut of susceptible Bombyx moristrain (306) at 48 h, but BmAdipoR mRNA quantity increased significantly at 72 h. We concluded that BmAdipoR gene was a membrane protein ubiquitously expressed in Bombyx moritissues and that its expression was altered by treating with BmNPV.
EN
The telomere structures in Bombyx mori are thought to be maintained mainly by the transposition of the specialized telomeric retroelements SART and TRAS. The silkworm genome has telomeric TTAGG repeats and telomerase, but this telomerase displays little or no activity. Here, we report that the transcription of the telomeric retroelements SART1 and TRAS1 is suppressed by the silkworm Piwi subfamily proteins BmAgo3 and Siwi. The silkworm Piwi subfamily was found to be expressed predominantly in the gonads and early embryo, as in other model organisms, but in BmN4 cultured cells, these proteins formed granules that were separate from the nuage, which is a different behaviour pattern. The expression of TRAS1 was increased in BmN4 cells when BmAgo3 or Siwi were silenced by RNAi. Our results suggest that B. mori Piwi proteins are involved in regulating the transposition of telomeric retroelements, and that the functional piRNA pathway is conserved in BmN4 cultured cells.
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