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2 Journal of Applied Genetics

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Reproductive performance of a transgenic boar carrying the bovine growth hormone gene (bGH)

100%

Rejduch B. , Slota E. , Sysa P. , Rozycki M.

nr 3

337-341

The aim of this study was to obtain information on the possible influence of the bovine growth hormone gene (bGH) on gametogenesis and reproductive parameters of a 2-year-old Polish Landrace transgenic boar. The bGH gene construct had been introduced into the zygote of the boar with the use of the microinjection technique. On the basis of the available documentation we established that the fertility of the investigated transgenic (bGH) boar was low in comparison with other animals of the Polish Landrace breed, with a poor libido, ineffective matings and, on average, 3 live piglets less per litter. Samples of testis tissue from the boar were obtained after castration. In total, we observed 100 spermatocytes and all of them had normally paired bivalents. It is possible that the boar?s lower fertility was caused by some, as yet unknown factor.

A new case of reciprocal translocation t(10;13)(q16;q21) diagnosed in an AI boar

88%

Danielak-Czech B. , Slota E.

2007

tom 48

nr 4

379-382

A new case of reciprocal translocation t(10;13)(q16;q21) was detected in a hybrid boar (Large White ? Pietrain ? Duroc ? Hampshire) from an artificial insemination (AI) station. Altogether, 258 sires of 4 pure breeds as well as hybrid lines and crossbreeds were investigated. The diagnosis was based on classical cytogenetic examination following the standard protocols of lymphocyte cultures, Giemsa staining and G-, C- and Ag-I banding techniques. The population screening performed was an initial part of a long-term karyotype control system of boars kept at AI stations, which was started by the National Research Institute of Animal Production in Poland in 2007.