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Bioconversion of trans-cinnamic acid to L-phenylalanine by Rhodotorula sp.

100%

Gientka I. , Blazejak S. , Duszkiewicz-Reinhard W.

Biotechnologia

2006

nr 2

117-129

The synthesis of the aromatic amino acid L-phenylalanine has received considerable attention in recent years due to its increasing importance as precursor to the dipeptide sweetener aspartame. Phenylalanine ammonia lyase (PAL), which occurs in yeast, catalyzes the nonoxidative deamination of L-Phe to trans-cinnamic acid (tCA), has industrial application in the synthesis of L-Phe. Superior producers of PAL are Rhodotorula sp. PAL is induced in yeast cell by the presence of L-Phe, while glucose represses PAL synthesis. Different additives and conditions during inducing PAL: permeabilizing, reducing and stabilization agents, as well as pH and temperature during bioconversion targeting to higher productivity of L-Phe were discussed.

Bioconversion of D-sorbitole into L-sorbose with immobilized bacterial cells

100%

Walisch S. , Lipinski J.

Biotechnologia

1994

nr 4

164-170

Gluconobacter sp. cells were immobilized in three different carriers:agar, calcium alginate and k-carrageenan.In all the three cases we achieved good conversion of D-sorbitol into L-sorbitol.The best effects were obtained while using alginate as a carrier.The yield of bioconversion reached about 80% as soon aas after in 31 hours of the process,at pH=7.Using agar as a carrier, a similar yield (about 80%) was achieved during 43 hours of the process.Carrageenan used as a carrier gave a little worse results- the yield of about 70%.

Microbial production of ethanol from lignocellulosic wastes

100%

Szczodrak J. , Fiedoruk J.

Biotechnologia

1993

nr 3

145-156

Current trends in the production of ethanol from lingocellulosics are reviewed. Particular emphasis is laid on the preytreatment of the lignocellulose materials and their simultaneous saccharfication and fermentation to ethyl alcohol.