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EN
In the present study, the influence of bioaugmentation strategy on the co-treatment of 13% v/v reject water and municipal wastewater at a decreasing temperature was evaluated. The experiment was performed in two identical laboratory sequencing batch reactors with the active volume of 8 L. Each one was operated using a 12-hour cycle at sludge retention time of 3 d. The SBR A was bioaugmented with a mixture of wild-living bacteria and Archaea in a dose 0.25 ml. In turn, the comparative reactor (SBR B) was non-bioaugmented, the Archaea product was replaced with an equal volume of dechlorinated tap water. The experiment was divided into 3 phases, each with a different temperature range (20, 15 and 10°C). The temperature reduction did not adversely affect the process performance in the bioaugmented and non-bioaugmented system. Significant removal efficiencies were achieved in both SBRs. The major differences were observed for the COD content in the bioaugmented SBR at a temperature of 10°C. In this case, statistically lower concentrations in the effluent were observed in the bioaugmented system than in the non-bioaugmented one. Additionally, at a temperature of 10°C, an improved process performance was observed in the Archaea presence, but the differences were of no statistical significance.
EN
In this study, the effect of bioaugmentation on the sequencing batch reactor (SBR) performance while treating municipal wastewater and reject water under various temperature conditions was examined. Two lab-scale SBRs, each with the active volume of 8 L were used to perform this research. For bioaugmentation, a mixture of wildliving Bacteria and Archaea in a dose 0.25 mL was added to SBR A, while SBR B was a control one. Both reactors were fed with a mixture of wastewater and 13% v/v reject water. During the experiment, 5 phases with different temperature range were distinguished, each one lasted 14 d. The temperatures were investigated in 5°C increments, i.e. 20, 25, 30, 25 and 20°C. The obtained results indicated that in the bioaugmented reactor (SBR A), lower concentrations of NH4+–N, TSS, NO2-–N in effluent were observed as compared to control (SBR B). While for NH4+–N, regardless the temperature, the observed differences were statistically significant. Importantly, in both SBRs, the process was carried out in a stable way.
PL
Badano różne osady ściekowe po biologicznym oczyszczaniu pod kątem występowania w nich mikroorganizmów utleniających amoniak: Archaea (AOA) i bakterii (AOB). Wyniki badań wykazały, że badane mikroorganizmy występują w ściekowym osadzie nadmiernym, osadzie po dezintegracji i osadzie przefermentowanym. Wyniki wskazują na możliwość izolacji i namnażania Archaea w warunkach oczyszczalni komunalnej.
EN
Samples of concd. activated sludge (both non-disintegrated and disintegrated) were subjected to semi-quant. detection of selected genes using the polymerase chain reaction method, carried out in a thermocycler. The sludges were tested for the presence of archaea and bacteria oxidizing NH₃. The tested microorganisms were present in excess sewage sludge, sludge after disintegration and fermented sludge. The results indicated the possibility of isolation and multiplication of archaea in the conditions of a municipal sewage treatment plant.
EN
Methane is a most important greenhouse gas for planetary heating and it’s produced by methanogenic microorganisms as a metabolic byproduct and creates climate change. Methanogens are ancient organisms on earth found in anaerobic environments and methane is a key greenhouse gas concerned with methanogens. Therefore here is intense interest to writing this paper. A number of experiments have already conducted to study the methanogens in various environments such as rumen and intestinal system of animals, fresh water and marine sediments, swamps and marshes, hot springs, sludge digesters, and within anaerobic protozoa which utilize carbon dioxide in the presence of hydrogen and produce methane. The diversity of methanogens, belong to the domain Archaea and get involved in biological production of methane that catalyzes the degradation of organic compound as a part of global carbon cycle called methanogenesis. Majorly in this article we summaries the diversity of methanogens and their impact on global warming.
PL
Archebakterie stanowią bardzo złożoną, a przez to niezwykle interesującą grupę drobnoustrojów, zarówno ze względu na ich uzdolnienia do zasiedlania rozmaitych środowisk, w tym zwłaszcza ekstremalnych, jak również z powodu specyficznej budowy komponentów komórkowych. W ostatnich latach obserwuje się duży rozwój wiedzy na ich temat, co skutkuje pojawieniem się ogromnej puli mniej lub bardziej szczegółowych publikacji. W literaturze polskojęzycznej brakuje jednak prac przeglądowych ujmujących szerzej temat budowy i fizjologii tych organizmów. Niniejsza praca uzupełnia tę lukę, omawiając wybrane elementy komórek Archaea wyróżniające się unikalną budową lub funkcjami, takie jak warstwa S, ściana komórkowa, błona cytoplazmatyczna oraz różnorodne wypustki komórkowe. Ponadto zwrócono uwagę na niektóre niezwykłe aspekty metabolizmu archebakterii.
EN
Archaea are very complex and thus an extremely interesting group of microorganisms, both in terms of their abilities to colonize different environments, including extreme conditions, as well as due to the specific structure of cellular components. In recent years, a large development of knowledge about those organisms was observed, what results in the appearance of a huge pool of more or less detailed publications. However, Polish literature lacks the reviews wider endearing structure and physiology of those organisms. The present work complements that gap by discussing selected elements of Archaea cells of unique structure or functions, such as the S layer, the cell wall, cytoplasmic membrane and a variety of cellular appendages. In addition, some of the remarkable aspects of the archaebacteria metabolism were brought up.
EN
This paper examines the effect of Archaea on wastewater treatment in sequencing biological reactors (SBR). The research was carried out in two SBR reactors: a reactor with activated sludge bioaugmented with Archaea (microorganisms which constitute a third domain besides Bacteria and Eukaryotes); a reactor with conventional activated sludge was used as a control. Archaea were incubated in laboratory conditions as recommended by Archaea Solutions Inc. The research revealed that the time period required for the acclimation of the activated sludge in the presence of Archaea was twice as long as in the case of regular nitrifying activated sludge. The observed nitrogen and phosphorous removal from wastewater was achieved to a higher extent in sludge with Archaea and the sludge itself settled faster. The required concentration of oxygen in the reactor with Archaea was lower than in the classic set-up – this resulted in lowering the operating costs of the treatment plant. Furthermore, the denitrification process was significantly shorter and did not require nitrate nitrogen (V).
PL
W pracy przebadano wpływ archeanów na proces oczyszczania ścieków w cyklicznych reaktorach biologicznych. Badania przeprowadzono w dwóch reaktorach typu SBR, z których jeden był poziomem odniesienia (oczyszczanie w warunkach klasycznych), a w drugim oczyszczano ścieki osadem czynnym poddanym bioaugmentacji archeanami, mikroorganizmami stanowiącymi trzecią domenę obok bakterii i eukariontów. Archeany były inkubowane w warunkach laboratoryjnych wg metody zalecanej przez ArchaeaSolutions, Inc. Badania wykazały, że adaptacja osadu czynnego do pracy w obecności archeanów wymaga dwa razy dłuższego czasu, niż zwykły osad czynny, zdolny do efektywnej nitryfikacji. Efektywność usuwania związków azotu i fosforu ze ścieków w obecności archeanów jest większa, a osad czynny szybciej sedymentuje. Wymagane stężenie tlenu w reaktorze z archeanami jest mniejsze niż w układzie klasycznym, co zmniejsza koszty eksploatacji oczyszczalni. Proces denitryfikacji jest znacznie skrócony i nie wymaga obecności azotanów(V).
EN
The haloarchaeal diversity of a salt mine, a natural cave in central Anatolia, was investigated using convential microbiological and molecular biology methods. Eight halophilic archaeal isolates selected based on their colony morphology and whole cell protein profiles were taxonomically classified on the basis of their morphological, physiological, biochemical properties, polar lipid and protein profiles and 16S rDNA sequences. From the 16S rDNA sequences comparisons it was established that the isolates CH2, CH3 and CHC resembled Halorubrum saccharovorum by 98.8%, 98.9% and 99.5%, respectively. There was a 99.7% similarity between the isolate CH11 and Halobacterium noricense and 99.2% between the isolate CHA1 and Haloarcula argentinensis. The isolate CH8K and CH8B revealed a similarity rate of 99.8% and 99.3% to Halococcus dombrowskii, respectively. It was concluded that the isolates named CH2, CH3 and CHC were clustered in the genus Halorubrum and that CHA1 and CH7 in the genus Haloarcula, CH8K and CH8B in the genus Halococcus and CH11 in the genus Halobacterium.
EN
The 2',3'-cyclic phosphate termini are produced, as either intermediates or final products, during RNA cleavage by many different endoribonucleases. Likewise, ribozymes such as hammerheads, hairpins, or the hepatitis delta ribozyme, generate 2',3'-cyclic phosphate ends. Discovery of the RNA 3'-terminal phosphate cyclase has indicated that cyclic phosphate termini in RNA can also be produced by an entirely different mechanism. The RNA 3'-phosphate cyclase converts the 3'-terminal phosphate in RNA into the 2',3'-cyclic phosphodiester in the ATP-dependent reaction which involves formation of the covalent cyclase-AMP and the RNA-Ni3'pp5'A intermediates. The findings that several eukaryotic and prokaryotic RNA ligases require the 2',3'-cyclic phosphate for the ligation of RNA molecules raised a possibility that the RNA 3'-phosphate cyclase may have an anabolic function in RNA metabolism by generating terminal cyclic groups required for ligation. Recent cloning of a cDNA encoding the human cyclase indicated that genes encoding cyclase-like proteins are conserved among Eucarya, Bacteria, and Archaea. The protein encoded by the Escherichia coli gene was overexpressed and shown to have the RNA 3'-phosphate cyclase activity. This article reviews properties of the human and bacterial cyclases, their mechanism of action and substrate specificity. Possible biological functions of the enzymes are also discussed.
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