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EN
In this reported study, a direct high-performance thin-layer chromatographic (HPTLC) method was developed to qualitatively detect and quantitatively determine glycerol in Antarctic krill for the first time. This procedure was based on the extraction of glycerol by ultrasonic solvent extraction with anhydrous ethanol, silica-gel column chromatographic separation, HPTLC detection and quantification using methylene chloride–methanol (5:1, v/v) as the developing solvent and alkaline potassium permanganate as chromogenic agent. The content of glycerol was 1.3725 ± 0.218 mg/g in freeze-dried Antarctic krill. The structure of glycerol in the Antarctic krill was subsequently determined by gas chromatography–mass spectrometry (GC–MS) which verified the presence of the material in the krill. The HPTLC method exhibited excellent accuracy with a recovery of 90.1–103.3% and good precision with a relative standard deviation (RSD) of 1.59–4.84%. The results clearly exhibited the applicability of the proposed for quantifying glycerol in Antarctic krill.
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EN
Antarctic krill mainly inhabit the Antarctic Ocean, not far from Antarctica, especially the Weddell Sea, where krill is dense. Marine fisheries have reached new levels, but the topic of sustainable use of marine fishery resources is far from reaching the required levels. In order to study the sustainable development of the Antarctic krill environment, this paper studies the living environment and applicability of Antarctic krill based on system dynamics, and provides some references for the sustainable development of marine resources. Mentioned the use of case analysis method, literature analysis method and other methods to collect data, build a Model, and read and analyse a large number of related literatures through the literature survey method. The experimental results proved that the salinity has a significant effect on the survival rate of Antarctic krill (p < 0.05). When the salinity is 34, the molting frequency reaches its maximum value, which is 70 %. It is concluded that the ability of Antarctic krill to adapt to gradual changes in salinity is stronger than that of sudden changes in salinity, and the suitable salinity for survival is 30-42. With 34 as the basic salinity, when the salinity rises within a certain range, the molting rate of krill will increase, and as the salinity decreases, the molting rate will gradually decrease. This shows that improving the environmental resources of Antarctic krill is an effective method for improving salinity.
EN
From three krili products (powder, precipitate, and protein concentrate), 132 volatile compounds including 38 hydrocarbons, 18 alcohols, 16 aldehydes, 13 ketones, 22 nitric compounds, 10 fatty acids, 6 sulfuric compounds, and 11 other, have been isolated and identified using GC-MS, and distilling as well as extractive techniques. Total volatile compounds content (expressed as dry weight of the product) in krill protein concentrate, krill powder, and krill precipitate amounted to 75.41, 35.58, and 29.25 mg/kg, respectively. It was found that the specific smell of krill powder was due to: piperazine, 2-metyl-N-phenyl-propanoamide-2, dodecanoic acid amine, 4-methylthiazole, dimethyl trisulphide, pentadecanocarboxylic acid, 2,3-dihydroinden-lH-on-l, and heptadecanocarboxylic acid, and that of krill precipitate was due to nonadien-3,6-ol-1, methyl ester of 4,7,10- hexadecanotrienoic acid, 2,4-dimethyl-pentanol-2, tricyclodecanodimethanol, 3-dodecenylo-dihydro-2,5-furandion, heptenal-4, and octen-2-ol-1. It was further found that the specific smell of krill concentrate was due to: 4-methylpyrimidine, 4-methylpyridine, azulene and furandion derivatives, and methyl ester of 4,7,10-hexadecanotrienoic acid. Variations in qualitative and quantitative composition of volatile compounds in products under test were found to be due to differences in the process of production, in particular within parameters of heat treatment.
PL
Zbadano skład jakościowo-ilościowy lotnych związków występujących w mączce krylowej, precypitacie i białkowym koncentracie krylowym na podstawie analizy GC-MS
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