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  1. 6 Annals of Agricultural and Environmental Medicine
  2. 3 Acta Parasitologica
  3. 3 Annals of Parasitology
  4. 3 Weterynaria w Praktyce
  5. 2 Medycyna Weterynaryjna
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  1. 1 2020
  2. 2 2018
  3. 5 2016
  4. 3 2012
  5. 4 2009
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  1. 2 Adamska M.
  2. 2 Adaszek L.
  3. 2 Blanarova L.
  4. 2 Buczek A.
  5. 2 Kowalska D.
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1
Content available Clinical course of granulocytic anaplasmosis in hunting dogs
100%
Teodorowski O. , Winiarczyk S. , Debiak P. , Skrzypczak M. , Mazurek Ł. , Adaszek Ł. , Teodorowski O. , Winiarczyk S. , Debiak P. , Skrzypczak M. , Mazurek Ł. , Adaszek Ł.
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nr 2
2
Identification of Anaplasma phagocytophilum on the basis of a fragment of the 16S rDNA gene
88%
Rymaszewska A.
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nr 3-4
3
Content available Coincidence of three pathogens [Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti] in Ixodes ricinus ticks in the Lublin macroregion
63%
Wojcik-Fatla A. , Szymanska J. , Wdowiak L. , Buczek A. , Dutkiewicz J.
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nr 1
151-158
4
Prewalencja infekcji Anaplasma phagocytophilum i Borrelia burgdorferi s.l. u psów z poszczególnych województw Polski
63%
Polozowski A. , Pantchev N. , Piekarska J. , Szwedko A. , Kowalska D.
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nr 04
5
Wild boar (Sus scrofa) — reservoir host of Toxoplasma gondii, Neospora caninum and Anaplasma phagocytophilum in Slovakia
63%
Reiterova K. , Spilovska S. , Blanarova L. , Derdakova M. , Cobadiova A. , Hisira V.
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tom 61
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nr 2
6
Content available Anaplasma phagocytophilum infection of red foxes [Vulpes vulpes]
63%
Karbowiak G. , Vichova B. , Majlathova V. , Hapunik J. , Pet'ko B.
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nr 2
299-300
7
Nowe wiadomosci na temat anaplazmozy psow
63%
Alleman A. R. , Wamsley H. L.
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tom 10
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nr 2
5-10
8
Molecular screening for bacteria and protozoa in Great Cormorants (Phalacrocorax carbo sinensis) nesting in Slovakia, Central Europe
63%
Vichova B. , Reiterova K. , Spilovska S. , Blanarova L. , Hurnikova Z. , Turcekova L.
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nr 3
9
Nonspecific bacterial flora isolated from the body surface and inside Ixodes ricinus ticks
63%
Okla H. , Sosnowska M. , Jasik K. P. , Slodki J. , Wojtyczka R. D.
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nr 3
EN
Ixodes ricinus and other representatives of the order Ixodida are vectors of typical pathogens: Borrelia burgdorferi sensu lato, Anaplasma phagocytophilium, Babesia spp., a tick-borne encephalitis virus, and other microorganisms which are important from a medical and veterinary point of view. The presented study focuses on the verification of nonspecific bacterial flora of I. ricinus. We analyzed ticks collected in a forest region in Silesia, an industrial district in Poland. Methods of classical microbiology and biochemical assays (API 20 NE test, API Staph test and MICRONAUT System) were used for isolation and identification of microorganisms living on the body surface of I. ricinus and inside ticks. The results show the presence of various bacteria on the surface and inside ticks’ bodies. During the study, we isolated Acinetobacter lwoffi, Pseudomonas fluorescens, Aeromonas hydrophila, Achromobacter denitrificans, Alcaligenes faecalis, Stenotrophomonas maltophilia, Pseudomonas oryzihabitans, Micrococcus spp., Kocuria varians, Staphylococcus lentus, Kocuria kristinae, Streptococcus pneumo- niae, Rhizobium radiobacter, Staphylococcus xylosus. Majority of the isolated species are non-pathogenic environmental microorganisms, but some of the isolated bacterial strains could cause severe infections.
10
Content available Prevalence of infection with Francisella tularensis, Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in rodents from an endemic focus of tularemia in Bulgaria
63%
Christova I. , Gladnishka T.
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nr 1
EN
The prevalence of Francisella tularensis, Borrelia burgdorferi and Anaplasma phagocytophilum in rodents is a determinant for their role in maintaining pathogens in the environment. A total of 169 rodents, trapped in an endemic focus of tularemia, were examined by PCR to asses the frequency of infection with the etiological agents of tularemia, Lyme borreliosis and human granulocytic anaplasmosis. The overall prevalence of F. tularensis was 22%. In 6% of the black rats, F. tularensis was identified in coinfection with B. burgdorferi and in 3% in coinfection with A. phagocytophilum. B. burgdorferi and A. phagocytophilum were detected in 26% and 8% of the trapped rodents. Results obtained show the existence of an active natural focus of tularemia. The high level of coinfections indicated that the endemic focus is mixed and generates risk for multiple infections in humans. Further investigations are needed to reveal interactions between the pathogens in the infected animals.
11
Content available Incidence and prevalence of infection with Anaplasma phagocytophilum. Prospective study in healthy individuals exposed to ticks
63%
Grzeszczuk A. , Puzanowska B. , Miegoc H. , Prokopowicz D.
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nr 1
EN
The seroprevalence of human granulocytic anaplasmosis (former human granulocytic ehrlichiosis, HGE) has been documented in several studies, but little data exists on incidence rates in healthy individuals. In a prospective study, we tested 125 healthy adults (mean age 43 years) - workers of the Białowieża Primeval Forest National Park, north-eastern Poland - for Anaplasma phagocytophilum IgG antibodies using an indirect immunofluorescence antibody assay, and for Borrelia burgdorferi IgG with ELISA in a 12-month interval. The data concerning clinical symptoms consistent with human granulocytic anaplasmosis were collected using a standardized questionnaire. Of these 125 subjects, 9 were anti-A. phagocytophilum positive at the study entry. Four participants (3.2%) seroconverted from IgG negative to positive during the observation period. Three subjects (2.4%) converted from initially anti-A. phagocytophilum positive to negative. Specific IgG antibodies against Borrelia burgdorferi were detected in 27 (21.6%) individuals. Concurrence of Borrelia burgdorferi and Anaplasma phagocytophilum was observed in 3.2%, whereas 4% were Anaplasma phagocytophilum IgG positive and Borrelia burgdorferi IgG negative (not significant). Clinical symptoms associated with human granulocytic anaplasmosis were not present in seroconverting individuals. The obtained results confirm the occurrence of Anaplasma phagocytophilum infection in north-eastern Poland with asymptomatic clinical course.
12
Content available Wild game as a reservoir of Anaplasma phagocytophilum in North-Western Poland
63%
Adamska M. , Skotarczak B.
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tom 53
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nr 2
13
Content available The risk of exposure to Anaplasma phagocytophilum infection in Mid-Eastern Poland
63%
Tomasiewicz K. , Modrzewska R. , Buczek A. , Stanczak J. , Maciukajc J.
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nr 2
EN
Both the presence of Anaplasma phagocytophilum in ticks and the seroprevalence of human granulocytic anaplasmosis have been reported in different parts of Europe. There are few reports concerning this problem in Poland. The aim of the study was to assess the prevalence of Anaplasma phagocytophilum in ticks, and to detect antibodies against the HGE agent in serum of forest workers in the region of Mid-Eastern Poland. In our opinion, this should reflect the real probability of infection of people exposed to Ixodes tick bites. Seroactivity against Anaplasma phagocytophilum was detected in 20.6% of persons in the study group. Coexistence of anti-Borrelia burgdorferi was present in 84.6% of individuals seropositive to A. phagocytophilum. The PCR test identifying Anaplasma phagocytophilum was positive in 13.1% of overall tick samples. The highest prevalence of infection (45.7%) was found in female ticks. Anaplasmal DNA was detected in 4.5% of male ticks and only in 0.9% of nymphs. The results of our study confirmed the existence of A. phagocytophilum in the natural environment of Mid-Eastern Poland. As the risk for infection exists, it should call the attention of public health services to the possibility of an increasing number of patients with this disease.
14
Content available Detecting DNAs of Anaplasma phagocytophilum and Babesia in the blood of patients suspected of Lyme disease
63%
Hermanowska-Szpakowicz T. , Skotarczak B. , Kondrusik M. , Rymaszewska A. , Sawczuk M. , Maciejewska A. , Adamska M. , Pancewicz S. , Zajkowska J.
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nr 2
EN
Co-occurrence of granulocytic anaplasmosis, borreliosis and babesiosis in humans is a result of common vectors for the respective pathogens of these diseases, most commonly ticks from the genus Ixodes. Studies on ticks in Europe and also in Poland have shown that several pathogens may co-occur in individuals of I. ricnus. A total of 96 hospitalised patients infected or suspected of being infected with borreliosis were screened for A. phagocytophilum and Babesia sp. DNA. Positive results of PCRs for A. phagocytophilum DNA were obtained for 10 patients, 8 of whom were diagnosed with borreliosis earlier, and 4 of whom were diagnosed with tick-borne encephalitis (on the basis of serological studies of serum and cerebrospinal fluid). None of the 10 patients had clinical or biochemical markers of anaplasmosis, corroborating the existence of asymptomatic anaplasmosis or self-limiting course. in Europe. Similarly, Babesia DNA was not found in the blood of any of the patients. The results of the studies show that in diagnosing tick-borne diseases, clinical examinations should consider infection by two or even three tick-borne pathogens.
15
Anaplazmoza granulocytarna psów
51%
Zygner W.
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tom 05
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nr 4
16
Detection of Dirofilaria immitis antigen and antibodies against Anaplasma phagocytophilum, Borrelia burgdorferi and Ehrlichia canis in dogs from ten provinces of China
51%
Wang J. , Kelly P. , Zhang J. , Shi Z. , Song C. , Zheng X. , Zhang Y. , Hao Y. , Dong H. , El-Mahallawy H. S. , Xiong W. , Wang H. , Li J. , Zhang X. , Wang C.
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nr 2
17
Prewalencja infekcji Anaplasma phagocytophilum i Borrelia burgdorferi s.l. u psów z poszczególnych województw Polski
51%
Polozowski A. , Pantchev N. , Piekarska J. , Szwedko A. , Kowalska D.
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tom 21
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nr 03
18
Content available The comparative study of vector-borne pathogens in dogs and red foxes from the Mazovia voivodship
51%
Mierzejewska E. J. , Tolkacz K. , Dwuznik D. , Kakietek D. , Koczwarska J. , Bajer A.
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tom 62
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nr Suppl.
19
Comparison of the occurrence of tick-borne diseases in ticks collected from vegetation and animals in the same area
51%
Roczen-Karczmarz M. , Dudko P. , Demkowska-Kutrzepa M. , Meisner M. , Studzinska M. , Junkuszew A. , Sopinska A. , Tomczuk K.
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nr 08
EN
The aim of this study was to compare the prevalence of selected pathogens in ticks taken from cats and dogs and from vegetation in urban settlements. A study was conducted to estimate the distribution of Anaplasma phagocytophilum and Borrelia burgdorferi in adult Ixodes ricinus (236), Dermacentor reticulatus (237) and Ixodes hexagonus (3) ticks collected from animals in veterinary clinics (184) and from vegetation in urban settlements (292). The most numerous ticks collected from animals were Ixodes ricinus (73.9%), followed by Dermacentor reticulatus (24.5%) and Ixodes hexagonus (1.6%). A total of 65.8% of the ticks collected from vegetation were Dermacentor reticulatus and 30% were Ixodes ricinus. The arthropods removed from the animals were most commonly located around the neck (48.1%) and in the mouth area (17.1%). All ticks were analyzed by molecular techniques. The percentages of ticks positive for Borrelia burgdorferi and Anaplasma phagocytophilum among those collected from animals differed from the corresponding rates for ticks taken from vegetation in the same area. Anaplasma phagocytophilum was more common in ticks collected from vegetation (N = 137, or 47.20%) than in those from animals (N = 12, or 6.6%). Borrelia burgdorferi, as well, was more common in ticks collected from the vegetation (N = 96, or 32.9%) than in those from animals (N = 19, or 10.5%). The DNA of A. phagocytophilum and B. burgdorferi were detected in 30.4% and 22.8% of D. reticulatus ticks, respectively, and in 32.6% and 25.4% of I. ricinus ticks, respectively. The DNA of A. phagocytophilum was also found in one Ixodes hexagonus tick. Single infections were noted in 69 I. ricinus ticks, 56 D. reticulatus ticks and 1 I. hexagonus tick. Coinfections of A. phagocytophilum with B. burgdorferi were detected in 33 (14.0%) I. ricinus ticks and in 29 (12.24%) D. reticulatus ticks. Infected companion animals can form a reservoir for human tick-transmitted infectious agents. The monitoring of the pathogens transmitted by ticks is an important tool in preventing and combating infections transmitted to humans and animals.
20
Badania serologiczne w kierunku boreliozy i erlichiozy swin i krow na Lubelszczyznie
51%
Winiarczyk S. , Adaszek L. , Stefancikova A. , Pet'ko B. , Cislakova L. , Puchalski A.
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tom 63
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nr 05
561-565
EN
The aim of this paper was the detection of the specific antibodies anti-Borrelia and anti-Ehrlichia in the sera of 28 sick pigs and 29 sick cows from the area of the Lublin voivodeship. ELISA tests, and Western blot were conducted. In ELISA tests, 17.86% sera of swine were positive for spirochetes and 7.14% for Ehrlichia. In a bovine group 44.83% of sera specimens were positive for Borrelia, and 20.69% for Ehrlicha. All sera that were positive in ELISA tests for spirochetes were examined additionally in Western blot method for the presence of antibodies against Borrelia afzelii (13 bovine sera, and 5 swine sera). In 8 out of 13 bovine sera (61.54%) and in one out of 5 (20%) swine sera that were positive in the ELISA test, specific antibodies against Borrelia were detected. In all cases IgG immunoglobins reacted strongly with the antigens: 31, 39, 41, 66 kDa of B. afzelii. The results revealed a low specificity of the ELISA test for the detection of borreliosis and confirmed an endemic occurrence of Borrelia and Ehrlicha in the area of the Lublin voivodeship
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