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EN
The quantity and quality of these constituent may not usually be the same under certain conditions, so fruits sold in Owerri where heavy pollutant abound were analysed, three samples of each water melon, Pawpaw, pineapple and orange fruits were bought from Owerri commercial centre and their juices extracted and stored in screw cap bottles prior to analysis. The physiochemical parameters revealed that fruits were of good quality but showed positive and negative significant relationship correlation ships such as pH-FW (0.958), pH-SG (-0.025), TS-MC (-0.999), TA-FW (-0.987). Vitamin C content ranged from 13.14 ± 0.39 mg/100g to 75.46 ±1.72 mg/100g for water melon and orange respectively. In conclusion, fruits studied showed good quality and a good source of vitamin C and mineral content. Such analysis needs to be carried out periodically to ensure quality and create awareness to the public.
EN
The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L.) Merr.] and papaya (Carica papaya L.).The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own taxa and formed monophyletic clades. From the pathogenicity test, all isolates of D. zeae and E. mallotivora showed pathogenic reactions on their respective host plants. Genetic variability of these isolates was assessed using repetitive sequence-based PCR (rep-PCR) fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC) and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis indicated that all Dickeya and Erwinia isolates from the same species were grouped in the same main cluster. Similarity among the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among Dickeya and Erwinia isolates. But this method appears to be reliable for discriminating isolates from pineapple heart rot and papaya dieback.
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