Wyniki wyszukiwania - Biblioteka Nauki

1

Immunisation of carp [Cyprinus carpio L.] against motile Aeromonas

100%

Kozinska A. , Antychowicz J.

Bulletin of the Veterinary Institute in Puławy

|

2000

|

tom 44

|

nr 1

EN

The purpose of the study was to evaluate an optimum dose of Aeromonas hydrophila and Aeromonas sobria antigens for the vacccination of carp (Cyprinus carpio L.) and to compare the effectiveness of the vaccination with the antigens inactivated with formalin or thermally and given intraperitoneally (ip) or by immersion (imm). The dose was evaluated with the use of formalin antigens. Doses ranging from 3 x 10⁴ to 6 x 10⁸ cells were given by injection and doses of 3 x 10⁵ to 6 x 10⁶ cells/mL of water were administered in bath. Experiments were conducted at 12°C, 16°C, and 23°C ± 1°C . To compare the effectiveness of the antigens inactivated with formalin and thermally the two doses 3 x 10⁸ cells (ip) and 5- 6 x 10⁷cells/mL (imm) were used. The effect of immunisation was evaluated with challenge tests. The relative percentage of health (RPH) and relative percentage of survival (RPS) of fish were calculated. Doses of 3 x 10⁸ cells, and 5-6 x 10⁷ cells/mL given ip and imm, respectively, were considered the most effective irrespective of the water temperature. No marked differences were found between the administration of the antigen by injection and immersion. Fish manifested a significantly higher level of immunity after the administration of formalin antigen in comparison to that following the administration of the antigen inactivated thermally.

2

Aktywnosc hemolityczna i proteolityczna jako wskazniki patogennosci bakterii Aeromonas hydrophila i A.sobria dla karpi [Cyprinus carpio]

84%

Rogulska A. , Antychowicz J. , Zelazny J.

Medycyna Weterynaryjna

|

1994

|

tom 50

|

nr 02

55-58

EN

The pathogenicity of 68 strains of A. hydrophila and 44 of A. sobria was assessed in two-year-old carp (Cyprinus carpio L.). The fish were selected from erythro- dermatitis-free farms and were adapted to the laboratory environment for one month; then they were infected with a subepidermal bacterial suspension. The inoculum contained 107 bacterial cells in 0,2 ml of 0.85 per cent beffered salt solution (PBS). The haemolytic activity was tested on horse blood agar (a base with 5 per cent defibrinated blood) and the proteolytic activity on a base with 15 per cent of gelatine. The individual strains of A. hydrophila and A. sobria displayed different haemolytic and proteolytic activity and also different pathogenicity. Pronounced haemolytic activity was found in 93 per cent of highly pathogenic strains of A. hydrophila and in 87.5 per cent of distinctly pathogenic strains of A. sobria. The above properties were also noted in the 45 per cent and 10 per cent mildly pathogenic strains, respectively. However, these characteristics were also found in 15 per cent nonpathogenic strains of A. hydrophila. Combined pronounced haemolytic and proteolytic activity were displayed in 90 per cent of highly pathogenic strains of A. hydrophila and 87.5 per cent of A. sobria. Only one non-pathogenic strain of A. hydrophila also possessed a marked haemolytic and proteolytic activity.

3

Antibiotic susceptibility of Aeromonas hydrophila and A. sobria isolated from farmed carp [Cyprinus carpio L.]

84%

Guz L. , Kozinska A.

Bulletin of the Veterinary Institute in Puławy

|

2004

|

tom 48

|

nr 4

4

Immune cross reactions in carp [Cyprinus carpio L.] after single or double immunisation with Aeromonas hydrophila and Aeromonas sobria antigens

67%

Kozinska A. , Antychowicz J.

Bulletin of the Veterinary Institute in Puławy

|

2001

|

tom 45

|

nr 1

EN

The aim of the study was to evaluate the influence of monovalent A.hydrophila and A. sobria vaccine on the induction of the protective immunity against the heterologous strains of Aeromonas genus in carp (C. carpio L.). Additionally, the level of immunity in carp after single and double vaccinations was compared. Separate groups of carp were immunised with 1S-95 (A.hydrophila) or 4R-96 (A.sobria) antigens by intrapesi to neal injection or by immersion. The immunisation efficiency was evaluated using challenge tests with various heterologous strains of Aeromonas genus. Carp revealed a partial immunity against the heterologous strains irrespective of the antigen kind and the route of vaccine administration. Fish immunised by immersion demonstrated a particularly high level of immunity. Antigen 1S-95 (A.hydrophila) protected more efficiently against another heterogenous strain of A.hydrophila than against A.sobria strain and vice versa. Double immunisation failed to increase the protective immunity as compared to that produced by a single immunisation.

5

Occurrence of agglutinins and precipitins to motile Aeromonas strains in normal serum of common carp [Cyprinus carpio L.] and their role in protection of the fish against these bacteria

67%

Kozinska A.

Bulletin of the Veterinary Institute in Puławy

|

2001

|

tom 45

|

nr 2

EN

The relationship between the prevalence of normal serum agglutinins and precipitins to Aeromonas hydrophila and A. veronii biotype sobria in carp and the sensitivity of the fish to infection with these bacteria was studied. The presence of these antibodies depended on carp population and on individual fish. No correlation between the prevalence of agglutinins and the sensitivity of carp to infection was found. On the contrary, the fish having the precipitins to particular Aeromonas strains revealed significantly lower sensitivity to infections with the respective homologous strains than those without the precipitins.