The life cycle of Acanthostomum burminis (Bhalerao, 1926) Bhalerao, 1936 (Trematoda, Acanthostomidae) infecting Xenochrophis piscator has been elucidated. Life cycle stages were successfully reared in the laboratory. The life cycle, from cercaria to adult, took more than two months to complete. Pleurolophocercous cercariae were found in the thiarid snail Thiara tuberculata. Metacercariae occurred in fin rays of several species of freshwater fishes. Snakes became infected when fed on fishes containing metacercariae. The prepatent period is 28 days. Successive stages in the development of metacercariae and adults were followed.
Acanthostomoides apophalliformis Szidat, 1956 is redescribed and its distribution, prevalence and mean intensity of infection in different Patagonian lakes is included. Mature adults were found mainly in the digestive tract of Percichthys trucha, rarely in Oncorhynchus mykiss and Diplomystes viedmensis (new host record). The metacercaria parasitised mainly the liver of Galaxias maculatus and G. platei. Opistorchioid cercariae emerged from Heleobia hatcheri. Experimental infections with cercana exposed to G. maculatus were successful and the immature metacercaria obtained were similar to those naturally found in the same host.
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