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Anthocyanis in suspension cultures orginating from different parts of sweetpotato plant

100%

Konczak-Islam I. , Yoshinaga M. , Nakatani M. , Yamakawa O.

Biotechnologia

1999

nr 3

143-153

Anthocyanin accumulating cell lines were established from storage root, leaf and stem explants of sweetpotato (Ipomoea batatas (L.) Lam.), cv. Ayamurasaki. The calli developed on MS basal medium enriched with 2,4-D at 27?C accumulated pigments in the dark. The storage root originated suspension culture generated the highest amount of total anthocyanins expressed as colour value (5.9) followed by the cultures originating from leaf (4.3) and stem (1.7). The cultures displayed similar anthocyanin profile regardless of source of explants. The major pigments derived from suspension cultures appeared with earlier retention time on ODS-column HPLC than the YGM pigments accumulated in vivo, which suggests that they are highly hydrophilic and have simpler chemical structure.

Cytogenetics, embryology and fertility of red clovere (Trifolium pratense L.) with short flowers and anthocyanin spots on the leaves

88%

Kazimierski T. , Kazimierska E. M.

Journal of Applied Genetics

1995

tom 36

nr 4

353-362

Anthocyanin spot on the leaves of red clover is inherited indepently of light spot and uniform leaf colour.The gene determining anthocyanin spot is probably localized on the other chromosomes than the gene determining light spot on the leaves.A short flower tube is also determined by a single recessive gene of a pleiotropic action.It stimultaneously causes reduction of pistil style, thickening of the endithecium layer in anthers - entails their indehiscence, pollen grain-agglutination and often deformation, no division of part of pollen grains into a vegetative and generative cells as well as germination of a single grains in pollen sacks.The changes in the structure of endothecium and pollen grains cause almost complete male and female infertility of plants with short flower tubes.

Regeneration of Oxalis triangularis from hairy roots and the influence of glutathione on anthocyanin production

75%

Wielanek M. , Urbanek H. , Dobras M.

Biotechnologia

2004

nr 2

260-266

The Oxalis triangularis transformed plants were regenerated from hairy roots induced by A. rhizogenes LBA 9402. In vitro shoot proliferation of the transformed roots-regenerated plants was 65% higher by than that of the non-transformed ones. In vivo the hairy root syndrome was observed for the transformed roots-regenerated plants. Anthocyanin content in the leaves of non-transformed plants was about 40% higher than in the transformed roots-regenerated ones. In the non-transformed plants, GSH (1,5 mM) enhanced anthocyanin production by 30% at the most, while in the transformed roots-regenerated plants the maximal increase in its production was found on the 2nd and 9th days after the treatment, it reached 230-320% and 750-600% of control, respectively.

Stimulation of anthocyanin production by two-phase growth system, in callus of Rudbeckia hirta

75%

Luczkiewicz M. , Cisowski W.

Biotechnologia

1999

nr 4

199-210

Biotechnological research to achieve and then increase anthocyanin production in callus tissue of R. hirta L., involved testing a number of growth media, modified with growth regulators. The evaluation of the growth of propagated tissues, their water contents and in particular their ability to produce anthocyanins, led to the development of an original two-phase growth system. This new system has the advantages of both growth-stimulating media and pigment production media. As a result of the research, callus tissue was obtained on a two-phase growth medium, which was made of modified Schenk-Hildebrandt medium (growth phase) and Miller's medium (production phase). The callus synthesised a 12-component anthocyanin compound complex, which constituted 2,18% of dry mass. This is a considerable amount compared to 0,28% in the natural plant. Phytochemical analysis (TLC, HPLC, PC, UV, 1H-NMR) of the anthocyanin complex isolated from callus produced with the two-phase system proved that the dominating compounds in the pigment complex were: cyanidin-3-0-(6-0-malonyl-b -D-glucopyranoside) and cyanidin-3-0-(b-D-glucopyranoside).

Secondary metsabolites of tissue of Lamiaceae species

51%

Skrzypczak-Pietraszek E. , Grzybek j.

Biotechnologia

1997

nr 2

93-110

This paper reviews information from last decade world literature on the occurence in tissue cultures of some Lamiaceae species of secondary metabolites.The introductory part of paper gives information on the occurence of the biological active secondary metabolites in the native plant species from Lamiaceae family.