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Infectious cDNA clones of plant viruses

100%

Sadowy E. , Zagorski W. , Hulanicka D.

Biotechnologia

1996

nr 3

60-68

Obtaining of infectious clones corresponding to the genomes of RNA viruses has greatly enhanced investigations.The different strategies employed to construct infectious clones from plant RNA virus and parameters affecting infectivity of such clones have been described.The system represent a powerful tool fo studying plant virus replication, short and long distance virus movement and the virus - host interactions.It may serve as autonomously replicating vectors for the expression of foreign genes in plants.

Genetically engineered potato resistant to potato leafroll virus infection

100%

Palucha A. , Chrzanowska M. , Zagorski W. , Hulanicka D.

Biotechnologia

1997

nr 4

38-47

Binary plasmids carrying different fragments of the coat protein gene of potato leafroll luteovirus were constructed for transformation of commercial potato cultivar Bzura. Several transgenic plant lines were obtained and characterised. The resistance of transgenic plants to the virus was tested by inoculation with aphids or grafting. Selected transformants expressing viral RNA were resistant to virus challenge by viruliferous aphids. Expression of the antisense RNA prevented virus infection even after grafting with scions from infected plants.