Wyniki wyszukiwania - Biblioteka Nauki

1

Rola fosfolipidów w procesie przepuszczalności błon

100%

Strosznajder J.

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tom 5

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nr 1

2

Nitric oxide in cerebral ischemia. Molecular mechanism of its action

100%

Strosznajder J.

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nr 5

3

Regulation of arachidonic acid release by enzyme(s) of rat brain cortex

63%

Samochocki M. , Strosznajder J.

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1990

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tom 37

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nr 1

4

Effect of 7-Nitroindazole on nitric oxide mediated biochemical and morphological alterations evoked by brain ischemia

63%

Chalimoniuk M. , Strosznajder J.

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1997

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tom 57

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nr 5

5

Reakcje ADP-rybozylacji bialek i ich znaczenie biologiczne w komorkach eukariotycznych

63%

Koladkiewicz I. , Strosznajder J.

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nr 3

265-275

6

63%

Chalimoniuk M. , Strosznajder J.

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1997

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tom 57

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nr 5

7

Brain ischemia increased high affinity binding of [3H]muscimol into synaptic plasma membrane receptor

63%

Samochocki M. , Strosznajder J.

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nr 4-5

8

Fosfolipidy inozytolowe, ich metabolizm i znaczenie w procesie przekazywania informacji

63%

Wikiel H. , Strosznajder J.

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tom 15

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nr 1

9

Effect of beta-amyloid peptides on cholinergic receptormediated calcium signaling in brain cortex synaptoneurosomes

51%

Samochocki M. , Chalimoniuk M. , Strosznajder J.

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1997

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tom 57

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nr 5

10

Receptor mediated second messengers formation and function in aged brain. Modulatory effect of amyloid beta peptides

51%

Strosznajder J. , Samochocki M. , Chalimoniuk M.

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1997

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tom 57

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nr 5

11

Improved analytical procedure for the measurements of selected biogenic polyamines by HPLC following dabsyl derivatisation in brain tissue

51%

Głód B. K. , Albrecht J. , Hilgier W. , Strosznajder J.

Chemia Analityczna

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2000

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tom Vol. 45, No. 1

27-33

EN

We present two improvements of a routine method of analysis of biogenic polyamines after their derivatisation with dabsyl chloride. The use of borate buffer during dabvsylation offers much better reproducibility then bicarbonate one. Also trichloracetic acid extrac-tion of polyamines from biological samples was found to be superior to perchloric acid extraction. Our studies eliminate the possibility to determine together biogenic polya-mines and aminoacids using common extraction procedure.

PL

Przedstawiono dwa udoskonalenia rutynowej metody analizy poliamin biogennych po uzyskaniu pochodnych za pomocą chlorku dabsylu. Stwierdzono, że podczas dabsy-lowania odtwarzalność metody jest lepsza przy zastosowaniu buforu boranowego niż w przypadku buforu wodorowęglanowego. Również zastosowanie kwasu trichlorooctowego do ekstrakcji poliamin z próbek biologicznych okazało się korzystniejsze niż w przypadku ekstrakcji kwasem nadchlorowym. Badania nasze eliminują wspólne ozna-czenie aminokwasów i poliamin biogennych przy użyciu rutynowych metod.

12

51%

Strosznajder J. , Koladkiewicz I. , Chalimoniuk M. , Samochocki M.

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tom 58

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nr 2

13

Mitochondrial and nuclear targets of amyloid wita-evoked oxidative stress

45%

Jesko H. , Strosznajder J. , Cieslik M. , Cakala M. , Strosznajder R.

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nr 3

EN

Amyloid β (Aβ) is responsible for mitochondrial failure and biochemical alterations linked to Alzheimer`s disease (AD). To better understand mechanisms of Aβ toxicity we investigated its mitochondrial and nuclear targets, apoptosis-inducing factor (AIF) and Poly(ADP-ribose) polymerase-1 (PARP-1) in PC12 cells transfected with wild type (APPwt) or double Swedish-mutated human Amyloid Precursor Protein gene (APPsw) characterized by different Aβ concentrations. We found close relationship between Aβ level and cyclooxygenase (COX)- and lipoxygenase (LOX)-related free radical formation leading to p65/NF-κB nuclear translocation. COX and LOX inhibitors protected APPsw cells against p65 translocation. Aβ-evoked oxidative stress enhanced mitochondrial AIF level and inhibited PARP-1 in APPsw cells. Nitrosative stress evoked by 0.5 mM sodium nitroprusside (SNP) had no further effect on Aβ-altered PARP-1 activity and mitochondrial AIF level in APPsw cells. However, SNP evoked death of 70–80% of all cell types after 24 h. COX and LOX inhibitors had ameliorating effect in these conditions. Our data indicated that double Swedish mutation in APP signifi cantly increased cell vulnerability to oxidative stress. Enhanced mitochondrial AIF level and PARP-1 inhibition might be responsible for cell survival under oxidative stress evoked by accumulating Aβ in APPsw cells. COX and LOX inhibitors protected the cells against death caused by simultaneous Aβ toxicity and nitrosative stress.