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Detection of RNA1 and RNA2 of Soil-borne wheat mosaic virus in winter wheat grown from infected seeds

100%

Jezewska M. , Trzmiel K. , Zarzynska-Nowak A.

Journal of Plant Protection Research

2016

tom 56

nr 4

A Polish isolate of Soil-borne wheat mosaic virus (SBWMV-Pol1) was characterized by limited pathogenicity and a low concentration of virus particles in infected plant tissues. The aim of this research was to consider the possibility of seed-transmission dissemination of the virus. Seeds of winter wheat cv. Muszelka served as material for the studies. Two methods were involved in the diagnostics of seedlings grown from potentially infected seeds: enzyme-linked immunosorbent assay (ELISA), as the screening assay and immuno-capture-reverse transcription-polymerase chain reaction (IC-RT-PCR) for molecular confirmation of the infection. RNA1 and RNA2 of SBWMV-Pol1 were detected in 6 out of 1,410 plants submitted to diagnostic procedures. The possibility of seed transmission of SBWMV-Pol1 was discussed.

Czy to wirus?

100%

Zarzynska-Nowak A. , Hasiow-Jaroszewska B. , Borodynko-Filas N.

Warzywa i Owoce Miękkie

2019

nr 07

Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland

100%

Jezewska M. , Trzmiel K. , Zarzynska-Nowak A.

Journal of Plant Protection Research

2018

tom 58

nr 2

Water samples were collected from irrigation ditches and drainage canals surrounding fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed centrifugation and obtained pellets were used to perform biological assays. Viral identification involved biological, electron microscopic as well as molecular methods. The occurrence of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) was demonstrated in 12 of the 17 examined water sources. The molecular analysis results showed TMV and ToMV co-infections in the analysed water samples. To our knowledge, this is the first report of tobamoviruses being found in environmental water in Poland.

Detection and characterization of Chrysanthemum virus B (CVB) isolates in Poland

100%

Zarzynska-Nowak A. , Hasiow-Jaroszewska B. , Borodynko N. , Pospieszny H.

Progress in Plant Protection

2014

tom 54

nr 4

In 2013 and 2014 in the Plant Disease Clinic of Institute of Plant Protection – National Research Institute in Poznań, chrysanthemum plants showing symptoms of stunted growth, mosaic and malformation of leaves and abnormal pigmentation of flowers were tested. All plants were analyzed using biological, electron microscopy and molecular biology methods. The plants were examined for the presence of the following pathogens: Chrysanthemum virus B (CVB), Tomato aspermy virus (TAV), Chrysanthemum stunt viroid (CSVd) and Chrysanthemum chlorotic mottle viroid (CChMVd). The presence of CVB was confirmed in the samples collected from 15 plants. The isolates were genetically identical and shared high level of similarity with isolates from India and Japan.

W latach 2013–2014 w Klinice Chorób Roślin Instytutu Ochrony Roślin – Państwowego Instytutu Badawczego w Poznaniu testowano rośliny chryzantemy wykazujące objawy: zahamowania wzrostu, mozaiki i deformacji blaszek liściowych oraz nieprawidłowej pigmentacji kwiatów. Przeprowadzono testy biologiczne, obserwacje mikroskopowe oraz badania z zastosowaniem technik biologii molekularnej. Rośliny testowano na obecność patogenów takich, jak: wirus B chryzantemy (Chrysanthemum virus B, CVB), wirus aspermii pomidora (Tomato aspermy virus, TAV), wiroid karłowatości chryzantemy (Chrysanthemum stunt viroid, CSVd) oraz wiroid chlorotycznej plamistości chryzantemy (Chrysanthemum chlorotic mottle viroid, CChMVd). W 15 próbach wykryto jedynie wirusa B chryzantemy. Sekwencje nukleotydów genu białka płaszcza badanych izolatów CVB były identyczne. Analiza filogenetyczna wykazała podobieństwo genetyczne polskich izolatów CVB do izolatów z Indii i Japonii.