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EN
The paper presents the assessment of the water condition of the Łękawka stream according Water Framework Directive, carried out on the basis of research on benthic macroinvertebrates and the analysis of data on other indicators used in the monitoring of waters. Sampling of macroinvertebrates, taxonomic identification and calculation of the MMI_PL index were carried out in accordance with the Multi-Habitat Sampling (MHS) method according to the EU standard EN16150:2012, used in Poland for the monitoring of surface waters. Field studies were carried out in September 2022. From each site, 20 quantitative samples were taken from various fractions of the substrate present in the bottom, using a Surber net. Data on other biological indicators, physicochemical and hydrobiological parameters and chemical status were obtained from the resources of the State Environmental Monitoring, concerning the results of monitoring of rivers and dam reservoirs in Poland in 2016-2021. Studies of the invertebrate macrofauna of the Łękawka stream showed the occurrence of only nine families, dominated by taxa with a wide range of ecological tolerance. The final assessment of the ecological status of the Łękawka water body, according to the RDW guidelines, based on the results of monitoring studies, showed a bad ecological status (class V) of the ecosystem. The key in-fluence on this assessment, in accordance with the principle "the worst decides" was the value of the Polish Multimetric Ecological Quality Index MMI_PL, confirmed by the results of the hydromorphological index (HIR). The chemical status of the examined water body, determined on the basis of the results of monitoring priority substances in the field of water policy (a group of 45 indicators) and other pollutants (eight indicators), showed a "below good" class. Finally, on the basis of a comparison of the ecological and chemical status, the bad condition of the Łękawka stream was confirmed. The obtained results indicate a significant disruption in the functioning of the water ecosystem as well as a violation of the hydrological regime and morphological conditions of the streambed. It means that the achievement of the environmental goal set by the Water Framework Directive is very seriously threatened. Therefore, it is necessary to urgently regulate activities in the field of water and sewage management in the catchment area, regarding water intake and discharge of municipal and industrial sewage.
EN
The paper presents an assessment of the water quality of the Vistula river and its tributaries in selected municipalities in the Cieszyn district based on benthic macroinvertebrates. A total of 45 samples were taken on nine positions. Most of the collected individuals were identified to the family level, except: Heptageniidae which was diagnosed to the genus; Turbellaria and Oligochaeta identified into the class. The conducted research showed that 54 taxa of benthic macroinvertebrates occurred at the analyzed sites. Among them, 40 taxa have a bioindicative value. The most sensitive to pollution are mayflies from the family Ameletidae and caddisflies from the families Glossosomatidae, Leptoceridae and Beraeidae. However, their frequency and density at the study sites was very low. The families Ephemerellidae, Heptageniidae, Hydropsychidae and Polycentropodidae as well as annelids from the Oligochaeta class were much more frequent. Based on macrozoobenthos diversity data, two biological indices were calculated: BMWP-PL index and Margalef's biodiversity index. The values of these biological indicators was using to the classiffication of water into the appropriate quality class. The final classification showed that the examined sections of the Vistula river represents the I, II and III class of water quality what indicated high, good and moderate ecological potential/state. The first class of water quality in terms of both indices was recorded at the sites located in Ustroń, Skoczów and Drogomyśl. For these positions, the value of the BMWP-PL index was over 100, while the value of the Margalef's index was in the range of 6.26-7.17.The lowest, third class of water quality was found in Kiczyce and Ochaby Wielkie. In these places, the diversity of macroinvertebrates was the lowest, which was reflected in the low values of both studied indices (BMWP-PL: 40; 41, Margalef's index: 2.6; 3.37).
EN
Two species of mosses in relation to nitrogen metabolism were examined. This subject is little known in this group of plant. Investigations of nitrate reductase activity in green tissues of Brachythecium rutabulum (Hedw.) Schimp. and Atrichum undulatum (Hedw.) P.Beauv. were performed. The study was conducted in two localities: heavy contaminated waste tip Skalny located in Upper Silesia, and Blonia City Park in Bielsko-Biala which place was chosen as a control area. For both species high activity of the enzyme was detected. The nitrate reductase activity varied between 99 to 9093 nmol per g dry mass per hour for B. rutabulum and 265 to 5135 nmol per g d.m. per hour of nitrite synthesized for A. undulatum respectively on Skalny waste tip. In the control area the results varied between 747 to 1077 for B. rutabulum and 171 to 518 nmol per g d.m. per hour of nitrite synthesized for A. undulatum, respectively. The differences were statistically significant only between the two species but not between habitats probably due to high dispersion and small amount of replications. The levels of nitrate and nitrite in stream water in both areas were also measured. In the Skalny waste tip there were high and reached 1.66 mg · dm-3 of nitrite and 65 mg · dm-3 of nitrate, respectively. In the control area these amounts were lower and reach zero level for nitrite and 4.5 mg · dm-3 of nitrate, respectively.
PL
Dwa gatunki mchów badano pod kątem asymilacji azotu. To zagadnienie jest mało poznane u tej grupy roślin. Przeprowadzono badania aktywności reduktazy azotanowej w zielonych tkankach Brachythecium rutabulum (Hedw.) Schimp. i Atrichum undulatum (Hedw.) P.Beauv. Badania zostały wykonane w dwóch miejscach: na hałdzie powęglowej Skalny na Górnym Śląsku i w parku w dzielnicy Błonia w Bielsku-Białej, które zostało wybrane jako miejsce kontrolne. Dla obu gatunków stwierdzono wysoką aktywność enzymu. Aktywność reduktazy azotanowej wahała się od 99 do 9093 nmol na g suchej masy na godzinę dla B. rutabulum i 265 do 5135 nmol na g suchej masy na godzinę azotynu syntetyzowanego u A. undulatum na hałdzie Skalny. W miejscu kontrolnym wyniki wahały się od 747 do 1077 dla B. rutabulum i 171 do 518 nmol na g sm/h u A. undulatum. Różnice były statystycznie znaczące tylko pomiędzy gatunkami, ale nie pomiędzy typem siedliska prawdopodobnie ze względu na wysoką dyspersję i małą liczbę powtórzeń. Zmierzono także poziom azotanów i azotynów w strumieniu wody w obu miejscach. Na hałdzie Skalny zawartość była wysoka i osiągnęła odpowiednio: 1,66 mg dm-3 azotynu oraz 65 mg dm-3 azotanu. W miejscu kontrolnym wartości te były niższe i osiągnęły odpowiednio poziom zerowy dla azotynów i 4,5 mg dm-3 dla azotanu.
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