Wyniki wyszukiwania - Biblioteka Nauki

1

Zasady oceny oddziaływania na zdrowie w kontekście ochrony środowiska

100%

Targosz A.

Aura

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2021

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nr 12

2

Disappearance of lake Jelenino since the end of the XVIII century as an effect of anthropogenic transformations of the natural environment

67%

Ptak M. , Choinski A. , Strzelczak A. , Targosz A.

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tom 22

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nr 1

EN

This study presents the contribution of reclamation works to the disappearance of Lake Jelenino. On the basis of cartographic materials from the 19th and 20th centuries, as well as historical records, it was possible to establish that the lake of area 495.2 ha disappeared as a result of precise actions aimed at creating new settlements. Reclamation works proceeded in two stages. After the first stage, which took place in 1778-86, the area of Lake Jelenino was reduced by over 360 ha. In the 1970s the reservoir, whose area then amounted to 130 ha, was completely dried. Such a transformation of the natural environment was followed by social and economic changes – inhabitants of Jelenino village, who until then lived by fishing, in a short period of time retrained for agriculture and breeding.

3

Molecular alterations in fibroblasts exposed to Helicobacter pylori: a missing link in bacterial inflammation progressing into gastric carcinogenesis?

51%

Krzysiek-Maczka G. , Targosz A. , Ptak-Belowska A. , Korbut E. , Szczyrk U. , Strzalka M. , Brzozowski T.

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nr 1

4

Helicobacter pylori inhibits expression of heat shock protein 70 (HSP70) in human epithelial cell line. Importance of Cag A protein

51%

Targosz A. , Pierzchalski P. , Krawiec A. , Szczyrk U. , Brzozowski T. , Konturek S. J. , Pawlik W. W.

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nr 2

EN

Heat shock proteins (HSP) are crucial for the maintenance of cell integrity under normal cell growth and at pathophysiological conditions such as colonization of gastric mucosa by Helicobacter pylori (Hp). The effect of Hp on mRNA expression for HSP70 in the gastric epithelial cells in vitro has been little studied and remains inconclusive. In this study we attempted to determine the alterations in gene expression for HSP70 induced by two live strains of Hp in the epithelial MKN7 cells. The following Hp strains were employed; 1) Hp strain expressing cagA and vacA, and 2) cagA and vacA negative Hp strain without or with addition of exogenous recombinant protein CagA. MKN7 cells were incubated in a standard medium RPMI 1640 supplemented with 10% fetal bovine serum at 37°C with 5% CO2 and humidified atmosphere under basal condition or in a presence of Hp (1x109 CFU per dish) without or with the recombinant CagA (10 µg/ml of RPMI 1640 medium). After 3h, 24h and 48 h of incubation with Hp and in some experiments with the prolonged incubation time up to 72 h, the cells were harvested, the total cellular RNA was isolated and the expression of mRNA for HSP70 was determined by RT-PCR. The incubation of the MKN cells with CagA protein alone failed to affect significantly the expression of HSP70. In contrast, the strain Hp (cagA+, vacA+) inhibited in time-dependent manner the expression of mRNA for HSP70. When the MKN7 cells were coincubated with Hp (cagA+, vacA+) and exogenous CagA, the significant inhibition of the signal intensity for HSP70 mRNA was observed at 3 h and 24 h of incubation and these effects were followed by complete disappearance of the signal for HSP70 mRNA at 48 h. The incubation of MKN7 with Hp (cagA-, vacA-) also significantly attenuated the expression of HSP70 mRNA with the most pronounced inhibitory effect observed at 72 h of incubation with this Hp strain. Addition of the recombinant CagA to Hp (cagA-, vacA-) completely suppressed the expression of HSP70 at 48 h and 72 h after the end of incubation periods. We conclude that 1) both, Hp (cagA+, vacA+) and Hp (cagA-, vacA-) inhibit expression of HSP70 in MKN7 human gastric epithelial cells independently of the presence or absence of cagA gene, and that 2) recombinant CagA protein may exert biological activity in vitro via acceleration of inhibitory effect of Hp negative for Cag A and VacA on HSP70 expression in epithelial cells infected with this bacteria.

5

Role of curcumin in protection of gastric mucosa against stress-induced gastric mucosal damage. Involvement of hypoacidity, vasoactive mediators and sensory neuropeptides

43%

Czekaj R. , Majka J. , Ptak-Bielowska A. , Szlachcic A. , Targosz A. , Magierowska K. , Strzalka M. , Magierowski M. , Brzozowski T.

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2016

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tom 67

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nr 2

6

Association of the presence the Helicobacter pylori in the oral cavity and in the stomach

43%

Czesnikiewicz-Guzik M. , Karczewska E. , Bielanski W. , Guzik T. J. , Kapera P. , Targosz A. , Konturek S. J. , Loster B.

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nr 2

105-115

EN

Helicobacter pylori is a gram-negative, microaerophilic rod-shaped bacteria that lives beneath the gastric mucous layer, on the surface of epithelial cells. Stomach infection with this organism causes inflammation of the gastric mucosa, which can lead to gastritis, duodenal or gastric ulcer and even in rare cases to gastric carcinoma or MALT lymphoma. Approximately 50% of the world's population is believed to be infected with H. pylori. Most infections is probably acquired in childhood, but the exact route of transmission is unknown. It has been speculated that dental plaque might harbour Helicobacter pylori and, therefore, might be a source of gastric infection. In order to address this issue we studied the relationships between oral and gastric infections with H. pylori in 100 subjects. Methods: Gastric H. pylori infection was determined by 13C-urea breath test (UBT) and the presence of the bacteria in oral cavity was monitored by the culture from the saliva and from dental plaque. Results: H. pylori was found in the stomach in 51% of studied individuals, while oral H. pylori was found in 54% (in saliva) and in 48.3% (in gingival pockets), the difference was not statistical significant (p=NS). Interestingly, anti-Hp IgA was found in 84% of studied individuals. No relationship was found between the presence of the bacteria in the oral cavity and the H. pylori gastric infection. 54.9% of subjects with stomach infection showed concomitant presence of H. pylori in saliva. 53.2% of examined subjects with negative UBT-test revealed the presence of H. pylori in culture from the saliva. The X2 value of relationship between UBT and culture H pylori in saliva was 0.029 (p=0.9). Similarly, no relationship was found between the presence of H. pylori in the stomach and in the dental plaque (X2=0.6; p=0.4). As expected, the presence of H. pylori in the dental plaque was significantly correlated with the presence of bacteria in the saliva (X2=18.4; p=0.0002). We also compared the presence of H. pylori in the saliva of patients with and without teeth. The cultured H. pylori was found in 63.7% of patients without teeth and in 52.9% of patients with teeth. This indicates that the presence of teeth does not seem to affect the occurrence of H. pylori in saliva. We conclude that oral cavity contamination with of H. pylori occurs at similar degree to that in the stomach. However, there was no significant correlation between the occurrence of H. pylori in the stomach and in the oral cavity indicating that other factors, like susceptibility to infection due to acid environment in the stomach may be the major factor in gastric infection with that bacteria, while oral cavity may serve only as transient food-related contamination without clear relation to gastric infection.

7

Asymmetric dimethylarginine, an endogenous inhibitor of nitric oxide synthase, interacts with gastric oxidative metabolism and enhances stress-induced gastric lesions

34%

Kwiecien S. , Ptak-Bielowska A. , Krzysiek-Maczka G. , Targosz A. , Jasnos K. , Magierowski M. , Szczyrk U. , Brzozowski B. , Konturek S. J. , Konturek P. C. , Brzozowski T.

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nr 5