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2 2014

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Phenotypic and genotypic characteristics of antibiotic resistance of commensal Escherichia coli isolates from healthy pigs

100%

Mazurek J. , Bok E. , Pusz P. , Stosik M. , Baldy-Chudzik K.

nr 2

The objective of the study was to examine the characteristics of the resistance profiles of Escherichia coli isolated from healthy pigs from three farms in Western Poland. The sensitivity to 13 antimicrobial agents was tested by a disk diffusion method, and the presence of 13 resistance genes was determined by PCR. The majority of the isolates were multi-resistant. The most common multi-resistance patterns were streptomycin, trimethoprim, sulfisoxazole, ampicillin, tetracycline. Although some resistance genes, such as strA/strB, blaTEM, sul1, snl2, and tetA, were equally represented in isolates from each farm, differences in the distribution of tetB and tetC, hfrV, dhfrXII, and sull resistance genes were observed among the isolates from different farms. Approximately one-third (35.9%) of the isolates possessed a class 1 integron. The four major different variable regions of the class 1 integron contained streptomycin (aadAl, aadA2, and aadA5) and/or trimethoprim (dhfrI, dhfrV and dhfrXVIl), and/or sulphonamides (sull) resistance genes. The results of this study emphasise that uncontrolled use of antibiotics causes the development of resistance and provides the evidence of frequent occurrence of more than one gene encoding the resistance to the same antimicrobial agent in the multi-resistant strains.

Type 1 fimbriae in commensal Escherichia coli derived from healthy humans

100%

Pusz P. , Bok E. , Mazurek J. , Stosik M. , Baldy-Chudzik K.

nr 2

Type 1 fimbriae are one of the most important factors of Escherichia coli adaptation to different niches in the host. Our study indicated that the genetic marker - fimH gene occurred commonly in commensal E. coli derived from healthy humans but expression of the type 1 fimbriae was not observed. Identification of fim structural subunit genes (fimA-fimH) and recombinase fimE and fimB genes showed that many of the strains were carrying an incomplete set of genes and the genes expression study revealed that in strains with complete set of fim genes, the fimC gene, encoding the chaperone protein, was not expressed.