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EN
To understand the phytoremediation capability of Cd by Salix babylonica L. we studied Cd accumulation and translocation, antioxidant enzyme activities, lipid peroxidation, and soluble protein contents in S. babylonica exposed to 10, 50, and 100 μM Cd for 7, 14, 21, and 28 d. The results indicated that seedling growth was accelerated by 10 μM Cd, and significantly inhibited by 50 and 100 μM Cd. The contents of Fe and Mn decreased significantly. The superoxide dismutase (SOD) activity in roots exposed to Cd was significantly higher than that in leaves. The level of peroxidase (POD) was significantly higher than that of control except for the roots treated with 10 and 50 μM Cd on day 28. POD activity in leaves was lower than that in roots. The level of catalase (CAT) was significantly lower than that of control. At 100 μM Cd, malondialdehyde (MDA) content increased significantly during the whole experiment. 50 μM Cd could induce high content of MDA in leaves. In general, the contents of hydrogen peroxide (H₂O₂), superoxide anion (O₂·-), and soluble protein showed an increasing trend. S. babylonica could be an efficient phytoextraction plant as it had considerable ability to accumulate Cd.
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EN
Hydraulic characteristics and contaminant removal effect in a vertical subsurface-flow constructed wetland were investigated by establishing a numerical model. The results demonstrated that retardation factor decreased with time while wetland substrate was gradually filled with the importation of contaminated water until reaching the saturation state. The flow velocity increased with time when the substrate was not on saturated condition, and decreased rapidly after saturation. The process of the substrate reaching saturation state was layered and gradual. The increased rate of effective saturation in substrate was less than that of the post-period: the higher the saturation level, the more easily water flowed. When the substrate was not completely saturated, pollutant concentration increased sharply to the max value. After the substrate was at the saturation state, concentrations of contaminants decreased slowly with time as adsorption within the solid particles and biodegradation. At the early stage the retardation factor gradually decreased as water depth increased, resulting in a close relationship between effective saturation and the retardation factor. With the increase of porosity and partition coefficient and decrease of degradation rate, hydraulic efficiency of the vertical subsurface-flow constructed wetland lessened.
EN
Salix matsudana Koidz was exposed to different concentrations of Cd (0, 10, 50, and 100 µmol/L) to study the effects of Cd on mineral metabolism and antioxidant enzyme activities. The results showed that plant height and root length were inhibited by 50 and 100 µmol/L Cd, except the one under 10 µmol/L Cd treatment. The Cd content accumulated in different organs of S. matsudana, gradually increasing with increased Cd concentrations and prolonged treatment times. The root was the main organ for absorbing and accumulating Cd. Cd inhibited the accumulation of Fe, Zn, Mn, and Cu. In addition, the activities of antioxidant enzymes and the contents of reactive oxygen species were also changed by different concentrations of Cd. The results obtained here can provide scientific and objective data for the use of S. matsudana in the remediation of Cd-contaminated soil.
EN
The digital holographic phase microscopy (DHPM) technique which has been proposed for cellular morphology and dynamic analysis yielded highly desirable results. However, for nucleated cells (especially white blood cells (WBCs)), their submicroscopic structure has not yet been deconstructed through a phase unwrapping method due to the heterogeneity of an internal phase. By analyzing the phase heterogeneity of subclasses of WBCs, the typical phase models of them are built first in this paper; using the simulation method, the wrapped phase distributions of these models are obtained. However, by optimizing the wrapped phase maps and analyzing the relationships between them and typical blood cells, their features are selected and extracted. Then the models built are sorted out from each other successfully without unwrapping via analyzing these extracted features, which provides a valuable approach and technological base for the classification and identification of blood cells.
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