Wyniki wyszukiwania - Biblioteka Nauki

1

Attempts at the further characterization of exoprotease from Pseudomonas sp. strain S9

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Sklodowska A. , Matlakowska R.

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nr 2

2

The role of microorganisms in dispersion of thallium compounds in the environment

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Sklodowska A. , Matlakowska R.

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tom 53

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nr 4

EN

Thallium is a highly toxic element and very rarely studied in the context of environmental hazards connected with zinc and non-ferrous metal industry. Microorganisms naturally existing in post-flotation and smelt wastes can participate in thallium release from waste deposits and can contribute to its dispersion in the environment. Twenty-one isolates were obtained from wastes of a non-ferrous smelter in Southern Poland characterised by high heavy metal contamination. Ten isolates showed high activity in thallium leaching from wastes (post-flotation and smelt wastes) as well as from pure thallous sulphide. Additionally, cadmium and lead were bioleached from wastes. The isolated bacteria indicated thallium resistance at a concentration up to 100 mg/l and some of them were able to survive in good condition at a concentration of up to 4 g/l. The same bacteria were isolated from rivers and wastewater in this region. A preliminary characterisation of isolates was performed. It was shown that some petroleum products i.e. asphalt-base crude occasionally used for waste immobilisation at the edge of pond or flotation surfactants partially stopped the activity of sulphide oxidising bacteria.

3

Microbial transformations of fossil organic matter of kupferschiefer black shale – elements mobilization from metalloorganic compounds and metalloporphyrins by a community of indigenous microorganisms

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Matlakowska R. , Ruszkowski D. , Sklodowska A.

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tom Vol. 49, iss. 1

223--231

EN

The presented article describes the role of community of indigenous microorganisms occurring in the Kupferschiefer black shale in elements mobilization from fossil organic matter of the deposit. The short description of metalloorganic compounds and metalloporphyrins extracted from black shale was presented as well as the characterization of indigenous microorganisms. The processes of fossil organic matter colonization, biofilm development and elements mobilization accompanying bacterial growth was summarized.

4

Extracellular elements-mobilizing compounds produced by consortium of indigenous bacteria isolated from kupferschiefer black shale – implication for metals biorecovery from neutral and alkaline polymetallic ores

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Matlakowska R. , Wlodarczyk A. , Slominska B. , Sklodowska A.

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2014

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tom Vol. 50, iss. 1

87--96

EN

Extracellular compounds produced by a consortium of indigenous bacteria interact with the polymetallic Kupferschiefer black shale and implicate for elements mobilization and/or complexation. The extracellular compounds were identified by GC-MS as carboxylic acids, but also aromatic acids and alcohols. Due to their action in batch experiment 16 elements were mobilized from black shale. Among mobilized elements precious metals (Cu, Ni, Co, V) as well as toxic heavy metals (As) were determined. Extracellular metabolites produced by studied consortium may be utilized in non-contact biorecovery of precious metals from neutral or slightly alkaline ores, and in the bioremediation of heavy metalcontaminated sites.

5

Geomikrobiologia podziemnych środowisk kopalnianych monokliny przedsudeckiej

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Bąkowska A. , Karlicki M. , Włodarczyk A. , Matlakowska R.

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tom nr 469

201--216

PL

W wyniku działalności górniczej na obszarze monokliny przedsudeckiej powstało unikalne środowisko podziemne, w którym żyją mikroorganizmy – bakterie, archeony i grzyby. Są to przede wszystkim tzw. litobionty, dla których miedzionośny łupek bitumiczny zwany Kupferschiefer jest źródłem węgla i energii oraz makro- i mikroelementów. Wśród mikroorganizmów zasiedlających podziemne kopalnie wykryto wiele ciekawych rodzajów i gatunków wykazujących przystosowanie do tego wyjątkowego środowiska. Szczególnie ciekawe są zespoły mikroorganizmów, które reprezentują różnorodne strategie metaboliczne. Są to m.in. mikroorganizmy, które wykorzystują związki organiczne (organoheterotrofy), ale także takie, które mogą żyć korzystając jedynie ze związków mineralnych (chemolitoautotrofy), mikroorganizmy produkujące metan (metanogeny) oraz takie, które metan rozkładają (metanotrofy). Ponadto w kopalniach miedzi występują mikroorganizmy oporne na metale ciężkie oraz tolerujące duże zasolenie. Mikroorganizmy odgrywają ważną rolę w transformacji łupka bitumicznego Kupferschiefer, wpływają na jego skład geochemiczny i właściwości fizykochemiczne, a także decydują o składzie chemicznym wód podziemnych. Aktywność metaboliczna mikroorganizmów powoduje bioutlenienie kopalnej materii organicznej, w tym kerogenu oraz bioutlenienie minerałów siarczkowych. W wyniku tych procesów powstaje szereg utlenionych związków organicznych i nieorganicznych. Wśród nich są produkty degradacji materii organicznej, takie jak: alkohole, kwasy, ketony, aldehydy, oraz wtórne związki nieorganiczne, w tym liczne biominerały (m.in. siarczany). Część z tych związków ulega mobilizacji do wód podziemnych, część immobilizacji w formie osadów naskalnych.

EN

The unique underground environment developed in the area of Fore-Sudetic Monocline as a result of mining activity. This environment is inhabited by bacteria, archaea and fungi. They are mainly lithobionts for which copper-bearing (Kupferschiefer) black shale is the source of carbon and energy as well as macro- and microelements. Among them, many interesting genera and species adapted to this unique environment were found. Particularly interesting are microbial communities that represent a variety of metabolic strategies: microorganisms using organic compounds (organoheterotrophs), utilizing only mineral compounds (chemolitoautotrophs), producing methane (methanogens) and degrading it (methanotrophs). Additionally, underground mines inhabit microorganisms resistant to heavy metals and high salinity. Microorganisms play an important role in the transformation of Kupferschiefer, affecting its geochemical composition and physicochemical properties, and the groundwater chemistry. Microbial metabolic activity leads to biooxidation of fossil organic matter (including kerogen) and sulphide minerals. As a result of these processes a number of oxidized organic and inorganic compounds are formed. They are products of organic matter degradation, such as alcohols, organic acids, ketones and aldehydes as well as secondary inorganic compounds, including numerous biominerals (e.g., sulphates). Some of these compounds are mobilized to groundwater; some are immobilized in the form of sediments.

6

The growth, ferrous iron oxidation and ultrastructure of Acidithiobacillus ferrooxidans in the presence of dibutyl phthalate

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Matlakowska R. , Skudlarska E. , Sklodowska A.

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nr 3

EN

The iron-oxidizing bacteria Acidithiobacillus ferrooxidans is an example of strictly chemolitotrophic extremophile occurring in acidic environments. The prime niche of these microorganisms is an environment with low pH and high concentrations of iron, sulfide minerals or sulfur. Besides these environments, A. ferrooxidans is also isolated from heavy metal contaminated environments such as soil and sewage sludge and is known to be useful in bioremediation processes of these environments. In the current study, the influence of dibutyl phthalate on the growth, activity and ultrastructure of A. ferrooxidans ATCC19859 was shown. The presence of dibutyl phthalate in 9K medium did not influence A. ferrooxidans growth or ability to oxidize ferrous iron although changes in growth medium were accompanied by changes in the protein expression profiles of periplasmic fractions and remarkable changes in ultrastructure of the cell.