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tom 62
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nr 02
207-211
EN
Illnesses of the genital tracts, often complicated with inflammation and degenerative processes in the endometrium and disturbances in ovarian function, reduce fertility in mares. The aim of the study was to estimate ovarian activity and assess intranuclear estrogen receptors in the endometrium of mares suffering from endometriosis. The study was performed prior to the breeding season (December.January) on 13 Arabian mares suffering from average to highly advanced degenerative changes in the endometrium. Ovarian ultrasonography and estimations of progesterone concentration in serum were performed three times over a 2 week period (examinations: I, II, III). Segments of endometrium were collected twice (at follicle sizes < 20 mm and > 25 mm) by biopsy and the quantity of intranuclear estrogen receptors were estimated in each layer of the endometrium. An ovarian cycle culminating with ovulation occurred in most of the mares over a period of 29 days (8 animals, 62%). The remaining 5 animals (38%) displayed: atresia of the dominant follicle (2 mares), anovulatory hemorrhagic follicles (2 mares), and persistent corpus luteum (1 mare). The average concentration of progesterone serum in two examinations (I and III) clearly exceeded the value of 1 ng/ml (p ≤ 0, 05 between I and II examinations). Stronger expressions and a greater score of immunoreactive estrogen receptors in each layer of the endometrium were noted when large follicles (p ≤ 0, 01) were found on the ovaries.
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tom 71
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nr 07
EN
Insulin-like growth factor I (IGF-I) is a polipeptyde hormone produced mainly by the liver in response to the endocrine growth hormone stimulus, but it is also secreted by multiple tissues for autocrine or paracrine purposes. IGF-I represents one of the most important growth regulators, playing a central role in fetal and neonatal growth. However, the role of IGF-I in the reproductive physiology of horses is still little known. Therefore, the aims of this work were 1) to evaluate the IGF-I serum concentration in mares during the first 4 days after parturition and in their newborn foals during the first 4 days of life, and 2) to determine whether the IGF-I concentration may be influenced by the type of parturition. Two groups of subjects were examined: 7 healthy mares and their foals born by spontaneous parturition (group F), and 10 healthy mares and their foals born by non-spontaneous parturition, requiring medical assistance (group P). From each animal, the first blood samples were collected within 30 min of birth, and then, daily, during 4 days after parturition. The samples were collected once a day at 8.00 a.m. IGF-I was analyzed by the radioimmunoassay method with the IGF-RIA-CT kit (BioSource, Belgium). The results revealed that the mares from the group P had a statistically significantly higher concentration of IGF-I compared with the mares from the group F (90.9 ± 7.02 vs. 40.9 ± 5.94 ng/ml, respectively, p ≤ 0.01). Similarly, the statistically higher values of this factor were found in the foals from the group P than in those from the group F (130 ± 8.59 vs. 83.1 ± 6.56 ng/ml, respectively, p ≤ 0.01). Furthermore, a positive correlation was found between the concentration of IGF-I in the blood serum of mares and their foals on the first day of the study (r = 0.50, p ≤ 0.05). In summary, disturbances in the course of parturition did not have a negative impact on the IGF-I level in either mares or their newborn foals.
EN
The article presents a case of a newborn foal (32 hours of life) with an acute abdominal pain. The foal was sent to the clinic after 24 hours of conservative treatment in the field. Physical examination revealed acute obstruction of the small intestine. The patient was admitted for emergency surgery. General multimodal anaesthesia with endotracheal tube placement and oxygen supplementation was performed. During medial laparotomy, about one meter of necrotic small intestine was resected, and the right ovary was removed. This surgical procedure was performed under general infusion multimodal anaesthesia with intratracheal administration of a mixture of oxygen and air. Postoperative recovery was uneventful, and no complications were observed. During the 5-month observation period, no disturbances in the somatic development and general condition of the patient were found.
EN
The aim of the study was to determine the immunological state of bitches suffering from pyometra. The study was performed on 87 bitches with open pyometra, 13 bitches with closed pyometra and 17 healthy control bitches. All bitches with pyometra were treated by ovario-hysterectomy. Prior to surgery, blood samples were collected from them to determine: w.b.c., r.b.c., Hb, Ht, differential w.b.c. A NBT reduction test and phagocytic index was also performed on the blood. In addition, the lysozyme activity, total protein level and globulin fraction were evaluated in the serum. C-reactive protein (CRP) was determined in 40 of the bitches. Uterus aspirates were collected from 68 bitches with open pyometra and 13 with closed pyometra for bacteriological examination. The study demonstrated that all immunological parameters in bitches with pyometra were lower compared to those of the control group. Only the CRP level was very high. In bitches with open pyometra the following aerobes were isolated: E. coli (78%), streptococci (13%), staphylococci (6%) and Pseudomonas aeruginosa (3%). No aerobes were isolated in bitches with closed pyometra.
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