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Effects of Saline and Other Peritoneal Lavage Solutions on the Morphology and Function of in Vitro Mesothelial Cells

100%

Winckiewicz M. , Połubińska A. , Staniszewski R. , Bręborowicz A.

2007

tom 79

nr 8

540-547

The aim of the study was to evaluate the effects of 0.9% NaCl and other solutions suitable for peritoneal lavage on in vitro viability and fibrinolytic activity of human peritoneal mesothelial cells.Material and methods. Mesothelial cells were isolated from the intra-operatively collected greater omentum specimens and then cultured. Subsequently, eight cultures (n=8) were incubated for six hours with M199 culture medium (control group) or culture medium with the addition of 0.9% NaCl, PD fluid Gambrosol-Trio 10 or Hanks' solution. Immediately after exposure to the studied solutions, the amount of LDH released by the cultured cells was determined. Then, cultured cells were incubated for the next 24 hours; after this period their metabolism and fibrinolytic activity were determined by means of IL-6, t-PA and PAI-1 levels. Levels of these substances were compared to the amount of total cellular protein that was determined simultaneously.Results. Hanks' solution showed no significant influence on cellular culture architecture and mesothelium metabolism. PD fluid Gambrosol-Trio 10 damaged mesothelial cell structure and function as manifested by an increased release of LDH and enhanced t-PA synthesis, as well as decreased production of IL-6 and PAI-1. Exposure to 0.9% NaCl solution was found to cause a disturbance in cellular adhesion without significant structural damage (marked changes in cellular morphology without increased LDH release) and significant metabolism impairment as presented by reduced production of t-PA, IL-6 and PAI-1.Conclusions. Saline solution and PD fluids should not be used for peritoneal lavage. Further investigations are required to identify the least harmful solution for mesothelial cells suitable for application in intra-operative peritoneal lavage.

Creating Digital Question Databases: Use of Self-Tests in Teaching Medical Subjects

88%

Kołodziejczak B. , Roszak M. , Ren-Kurc A. , Bręborowicz A. , Kowalewski W.

Studies in Logic, Grammar and Rhetoric

2015

tom 43

nr 1

211-227

Enhancement of teaching using digital materials is rapidly entering the world of medical studies. Creation of a self-learning environment supported with self-tests is received well, or even enthusiastically, among students. On the other hand, there is a relatively large group of opponents among university teachers, who do not see the need for changes in teaching and testing methodology to be made. This attitude may be surfacing as a result of anxiety connected with implementing new technologies in teaching medical subjects, as well as the belief that implementing new technologies does not have an immediate effect on learning quality. The authors of this article attempt to demonstrate that a thoughtful choice of e-learning platform facilitates the process of implementing online learning and testing aids in medical faculties. The second part of the article presents initial results of studies concerning the efficiency of learning enhanced with self-tests. Our analysis details the results of exams in pathophysiology taken by students of the medical faculty at the Poznan University of Medical Sciences. After the course, an evaluation survey was completed by 195 students concerning the quality of teaching with the use of the OLAT (Online Learning and Training) e-learning portal. It showed that students had positive attitudes toward learning with the use of online materials, particularly with regard to the use of self-tests, which allowed students to check their knowledge independently in exam-like conditions. The article that follows is targeted at those teachers who are interested in implementing a self-study and electronic knowledge evaluation environment for their courses, not necessarily in medical subjects.

E-learning Portal Tools for Medical Education

75%

Roszak M. , Kołodziejczak B. , Półjanowicz W. , Bręborowicz A. , Ren-Kurc A. , Kowalewski W.

Studies in Logic, Grammar and Rhetoric

2015

tom 43

nr 1

177-193

The necessary prerequisites for efficiently pursuing distance learning include carefully planned organization of work and resource management tools, typically found in an internet application – a distance learning portal. The portal application must meet a number of capacity and functionality requirements. Learning goals are pursued through implementation of appropriate tools available in the portal. In the article that follows, the authors attempt to analyze this aspect of medical education as utilized by blended learning and e-learning classes. The article presents a proposal for a set of tools to organize classes in an e-learning portal at a medical school. In addition, the article presents the opinions of students of the Poznan University of Medical Sciences and the Medical University of Bialystok who took part in distance education classes.