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Antibiotics resistance in Enterococcus isolates from poultry waste

100%

Cybulska K. , Krzyśko-Łupicka T.

Ecological Chemistry and Engineering. S

2020

tom Vol. 27, nr 2

305--316

The aim of the study was to evaluate the drug resistance of Enterococcus faecalis and Enterococcus faecium isolated from different types of poultry waste. The study material consisted of feather samples (duck, turkey, chicken), sludge and centrifuge sediment, originating from three poultry farms. The study was conducted in two stages; isolation and identification of Enterococcus bacteria from the waste and evaluation of their drug resistance using Kirby-Bauer method. Contamination of the poultry waste with Enterococcus isolates included E. faecium species (79 %) and E. faecalis (21 %). The most contaminated were sludge and sediment from the centrifuge as well as chicken feathers, irrespective of the place and time of sampling. Tested isolates showed multiple resistance and similar reaction to all antibiotics used in the study and E. faecalis strain was more resistant. Enterococcus isolates showed the highest resistance to streptogramins, carbapenems, fluoroquinones, aminoglycosides and penicillins, and the lowest for nitrofurantions and phenicols.

The effect of ash and compost on the content and bioaccumulation of selected heavy metals

80%

Możdżer E. , Cybulska K. , Krzyśko-Łupicka T. , Meller E.

Polish Journal of Chemical Technology

2019

tom Vol. 21, nr 2

20--25

Therefore the carried out study aimed at determination of the effect of high-calcium brown coal ash and compost being produced from municipal sewage sludge on the content and bioaccumulation of heavy metals in potato tubers, wheat grains and rapeseeds during a three-year period. Rapeseeds contained most Cd whereas wheat rains less. Potato tubers, wheat grains and rapeseeds contained more Mn, Ni and Zn in the fertilization objects with municipal sewage sludge with or without coal ash and compared to those where calcium carbonate or coal ash had been introduced into the soil at a dose corresponding to 1.5 Mg CaO · ha−1 at the beginning of this study. Differences in the Mn, Ni and Zn contents in test plants between the fertilization objects with sewage sludge of with and without addition of ash were not significant.

The bactericidal effect of extracts from Humulus lupulus l marynka variety on selected bacteria

80%

Rył B. , Sporek M. , Krzyśko-Łupicka T.

Ecological Chemistry and Engineering. A

2020

tom Vol. 27, nr 1-2

1--11

The aim of the research was to obtain extracts of Humulus lupulus L hop cones of the Marynka variety and to evaluate their antibacterial properties against three strains of gram-negative bacteria (Escherichia coli ATCC 10536, E. coli IPS and Pseudomonas aeruginosa ATCC 27853) and against two strains of gram-positive bacteria (Enterococcus hirae ATCC 10541 and Bacillus cereus ATCC 12826). The research material consisted of dried Marynka variety hop cones, from which extracts in the form essential oil (EO), infusion and decoction were obtained. EO was obtained in the process of hydrodistillation. The antibacterial activity of the extracts was evaluated by the plate-cylinder diffusion method against the tested gram-negative and gram-positive bacteria. Diverse antibacterial activity of water extracts of H. lupulus L hop cones of the Marynka variety (essential oil, infusion and decoction) on the tested bacteria was demonstrated, with higher sensitivity of gram-positive than gram-negative bacteria. The highest resistance to hop extracts was demonstrated by P. aeruginosa ATCC 27853. The best antibacterial properties were demonstrated by the essential oil at a concentration of 2.0 %. In the group of gram-positive bacteria, E. hirae ATCC 10541 was more sensitive to EO, and E. coli IPS in the group of gram-negative bacteria. In the case of B. cereus ATCC 12826, the sensitivity to infusions and decoctions of hop cones requires further investigation, as the obtained large zones of growth inhibition were unstable.