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Ocena zdolności tworzenia biofilmu P. Aeruginosa na próbkach stopu AZ31 uzyskanego w technologii SLM

100%

Pawlak A. , Szymczyk P. , Chlebus E. , Junka A. , Secewicz A.

Aktualne Problemy Biomechaniki

2015

tom z. 9

89--92

W pracy przedstawiono wyniki badań nad oceną zdolności tworzenia biofilmu szczepu bakterii P. Aeruginosa, na powierzchni próbek wyprodukowanych z lekkiego stopu magnezu - AZ31 w technologii SLM. Ilość kolonii bakteryjnych wyhodowanych w biofilnie, jest nawet 700x wyższa, w odniesieniu do skafoldów ze stopu Ti-6Al-7Nb, wyprodukowanych w tej samej technologii.

In this work, results of research on ability to form biofilm by P. Aeruginosa strains on AZ31 specimens manufactured in SLM technology are reported. The number of cells forming biofilm on specimens is even 700 times higher than on scaffolds manufactured from Ti-6Al-7Nb alloy by SLM technique.

The chemical digestion of Ti6Al7Nb scaffolds produced by Selective Laser Melting reduces significantly ability of Pseudomonas aeruginosa to form biofilm

72%

Junka A. F. , Szymczyk P. , Secewicz A. , Pawlak A. , Smutnicka D. , Ziółkowski G. , Bartoszewicz M. , Chlebus E.

Acta of Bioengineering and Biomechanics

2016

tom Vol. 18, no. 1

115--120

In our previous work we reported the impact of hydrofluoric and nitric acid used for chemical polishing of Ti-6Al-7Nb scaffolds on decrease of the number of Staphylococcus aureus biofilm forming cells. Herein, we tested impact of the aforementioned substances on biofilm of Gram-negative microorganism, Pseudomonas aeruginosa, dangerous pathogen responsible for plethora of implant-related infections. The Ti-6Al-7Nb scaffolds were manufactured using Selective Laser Melting method. Scaffolds were subjected to chemical polishing using a mixture of nitric acid and fluoride or left intact (control group). Pseudomonal biofilm was allowed to form on scaffolds for 24 hours and was removed by mechanical vortex shaking. The number of pseudomonal cells was estimated by means of quantitative culture and Scanning Electron Microscopy. The presence of nitric acid and fluoride on scaffold surfaces was assessed by means of IR and rentgen spetorscopy. Quantitative data were analysed using the Mann–Whitney test (P ≤ 0.05). Our results indicate that application of chemical polishing correlates with significant drop of biofilm-forming pseudomonal cells on the manufactured Ti-6Al-7Nb scaffolds ( p = 0.0133, Mann–Whitney test) compared to the number of biofilm-forming cells on non-polished scaffolds. As X-ray photoelectron spectroscopy revealed the presence of fluoride and nitrogen on the surface of scaffold, we speculate that drop of biofilm forming cells may be caused by biofilm-supressing activity of these two elements.

Use of the real time xCelligence system for purposes of medical microbiology

72%

Junka A. F. , Janczura A. , Smutnicka D. , Maczynska B. , Secewicz A. , Nowicka J. , Bartoszewicz M. , Gosciniak G.

Polish Journal of Microbiology

2012

tom 61

nr 3

Roche’s xCelligence impedance-measuring instrument is one of a few commercially available systems of such type. According to the best knowledge of authors, instrument was tested so far only for eukaryotic cell research. The aim of this work was to estimate xCELLigence suitability for the microbiological tests, including (i) measurement of morphological changes in eukaryotic cells as a result of bacterial toxin activity, (ii) measurement of bacterial biofilm formation and (iii) impact of antiseptics on the biofilm structure. To test the influence of bacterial LT enterotoxin on eukaryotic cell lines, Chinese Hamster Ovary (CHO) cell line and reference strain Escherichia coli ATTC 35401 were used. To investigate Roche’s instrument ability to measure biofilm formation and impact of antiseptics on its development, Staphylococcus aureus ATTC6538 reference strain was used. The data generated during the experiments indicate excellent ability of xCelligence instrument to detect cytopathic effect caused by bacterial LT endotoxin and to detect staphylococcal biofilm formation. However, interpretation of the results obtained during real-time measurement of antiseptic’s bactericidal activity against staphylococcal biofilm, caused many difficulties. xCelligence instrument can be used for real-time monitoring of morphological changes in CHO cells treated with bacterial LT enterotoxin and for real-time measurement of staphylococcal biofilm formation in vitro. Further investigation is necessary to confirm suitability of system to analyze antiseptic’s antimicrobial activity against biofilm in vitro.