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1 World News of Natural Sciences

1 2021

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Phytochemical Screening and Antimicrobial Activity of Ethanol and Methanol Extracts of Lantana camara Leaf

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Ezebo R. O. , Okonkwo C. C. , Ozoh C. N. , Nwankwo C. A. , Nwafor E. C. , Esimai B. G. , Achonye C. C. , Obienyem J. N.

tom 37

151-163

This study investigated the phytochemical composition and antimicrobial activities of ethanol and methanol leaf extracts of Lantana camara Linn against some clinical pathogens. The ethanol and methanol extracts were obtained by soaking each of the powdered leaf in each solvent. The soaked powdered leaf was allowed to stand for four days at room temperature and later filtered using Whatman filter paper. The filtrate was further concentrated using rotary evaporator and then freeze-dried. The minimum inhibitory concentration (MIC) of the ethanol and methanol leaf extracts was carried out using agar well diffusion method. The phytochemical analysis was done using standard techniques. Data were analysed using Analysis of Variance (ANOVA) to test for significance. Means were separated using Duncan’s Multiple Range Test (DMRT). The results of the antimicrobial activity revealed that V. cholerae was the most susceptible while E. coli was the most resistant to plant extracts. The phytochemicals present in the plant leaf had antimicrobial properties and may serve as a good substitute for resistant human pathogens.