Wyniki wyszukiwania - Biblioteka Nauki

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Immunohistochemical detection of tumour cell proliferation and intratumoural microvessel density in canine malignant mammary tumours

100%

Sennazli G. , Yildirim F. , Arun S. S. , Gurel A. , Sonmez K.

Bulletin of the Veterinary Institute in Puławy

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2015

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tom 59

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nr 2

EN

The objective of this study was to investigate the correlation between different histological types and grades of canine malignant mammary tumours, tumour cell proliferation and their angiogenic activity using immunohistochemical markers. Mammary tissue samples from 47 bitches with mammary cancer were evaluated. The expression of cellular proliferation marker Ki-67 and endothelial marker Von Willebrand's factor (vWF) were immunohistochemically demonstrated. The tumours with the highest Ki-67 and vWF expressions were found to share similar histomorphological features. Simple solid carcinoma had the highest levels of Ki-67, vWF, and higher histological grade while complex carcinomas, osteosarcomas, and carcinosarcomas had the lowest ones. The differences between the expressions of Ki-67 and vWF in different tumour types were considered to be of great importance in determination of biological behaviour and prognosis of these tumours. This study is one of the few studies that evaluate these differences among the subtypes of malignant canine mammary tumours.

2

Effects of tyrosine kinase inhibitor-masitinib mesylate on canine mammary tumour cell lines

86%

Ustun-Alkan F. , Bakirel T. , Ustuner O. , Anlas C. , Cinar S. , Yildirim F. , Gurel A.

Journal of Veterinary Research

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2021

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tom 65

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nr 3

3

An abattoir survey on bovine spongiform encephalopathy (BSE) in Turkey

86%

Yilmaz H. , Turan N. , Gurel A. , Gulcubuk A. , Sonmez K. , Bozkurt H. H. , Morgan K. L.

Polish Journal of Veterinary Sciences

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2007

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tom 10

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nr 4

EN

The aim of this study was to investigate Bovine Spongiform Encephalopathy (BSE) in Turkish cattle in the Marmara region which borders the European Union (EU). For this, cattle brought to abattoirs in Istanbul were analysed. The high risk group were selected and therefore 384 cattle above 2 years old were included in the study. They were primarily examined for the presence of any clinical signs of nervous system and also other clinical disorders. The whole brains were taken and analysed for the presence of vacuolar degeneration and prion protein by PLATELIA BSE test kit. Only 5 cattle were found to be nervous and showed aggressive behaviour. There were no cattle showing incoordination or other neurological disorders. Cysts were observed in 3 brains. Histopathologically, no vacuolar degeneration indicative of BSE was found in any cattle examined. However, in 8 brains, few vacuoles were observed in neurons in sections taken from the brain, cerebellum, medulla oblongata and medulla spinalis. Slight mononuclear cell infiltration in 9 brain, intensed mononuclear cell infiltration in 1 brain, haemorrhages in 5 brains and gliosis in 11 brains were also found. No infective prion was detected by ELISA in samples taken from 384 cattle brain.

4

First report on the molecular detection, phylogeny, virological and pathological investigations of Avibacterium paragallinarum in chickens in Turkey

58%

Umar S. , Ongor H. , Bayraktar E. , Ozturk Gurgen H. , Sigirci B. D. , Satir E. , Ozkan K. , Turan N. , Yilmaz A. , Gurel A. , Ak S. , Cetinkaya B. , Yilmaz H.

Medycyna Weterynaryjna

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2020

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tom 76

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nr 04

EN

Avibacterium paragallinarum is an important pathogen affecting the respiratory tract of chickens. There is a paucity of information on the molecular characteristics and pathology of A. paragallinarum in Turkish poultry flocks. In the present study, broiler and layer flocks (n = 2) suspected of viral infections with serious respiratory signs and significant mortality were visited. Chickens showed various disease signs and necropsy lesions, including purulent nasal discharge, respiratory distress, facial edema, sticky eyes, mucoid tracheitis, hemorrhagic inflammation of the infraorbital sinuses along with fibrinous mass and conjunctivitis. Histopathological lesions included loss of cilia along with necrosis and exfoliation of the superficial mucosal epithelium of the trachea, facial cellulitis, dermatitis, fibrinous plasmatic edema and infiltration of inflammatory cells, especially heterophils. A. paragallinarum was detected in tissue samples by species-specific polymerase chain reaction (PCR). The sequencing and phylogenetic analysis of the core region of the hemagglutinin (HA) gene revealed that Turkish strains detected here belonged to serotype A (serovar A1). They were related to strains reported from India (VRDC), the United States (0083), and Japan (0221), which are representatives of serovar A1. A homology of 88-90% was found between Indian strains and the Turkish strains detected in this study. Surprisingly, only vaccine strains of infectious bronchitis virus (IBV) were detected as a co-infecting agent in all samples positive for A. paragallinarum. Our findings suggest that A. paragallinarum may be an emerging pathogen in Turkish poultry flocks, and direct PCR may facilitate rapid diagnosis of infectious coryza. These results will also help to develop control strategies for A. paragallinarum.