Wyniki wyszukiwania - Biblioteka Nauki

1

A trial attempt to define the course of yersiniosis in experimentally infected pigs

100%

Platt-Samoraj A.

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nr 1

37-47

2

Immunomodulacja u psow - aktualny stan wiedzy

63%

Siwicki A. K. , Platt-Samoraj A.

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nr 3 Suplement - Psy

3

Zakażenia Yersinia enterocolitica u ludzi i zwierząt

63%

Platt-Samoraj A. , Szweda W.

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nr 09

4

Ekstensywność inwazji Toxoplasma gondii u kotów z terenu Olsztyna

63%

Michalski M. , Platt-Samoraj A.

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tom 50

5

Distribution of the ymoA and ystA genes and enterotoxins Yst production by Yersinia enterocolitica strains isolated from humans and pigs

51%

Bancerz-Kisiel A. , Szczerba-Turek A. , Platt-Samoraj A. , Szweda W.

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nr 4

EN

Yersinia (Y.) enterocolitica is the third etiological agent of human diarrhea in terms of the number of confirmed clinical cases. One of the important virulence markers is the yst gene which encodes the production of enterotoxins Yst (Yersinia stable toxins). However, not all strains with yst genes produce enterotoxins, what seems to be caused by the ymoA gene encoding the production of the YmoA protein inhibiting the expression of various genes. The purpose of our study was to evaluate the distribution of the ymoA and ystA genes and Yst production by Y. enterocolitica isolated from humans and pigs. All the studied strains obtained from pigs had the ystA gene which indicates that they belong to the group of strains commonly regarded as pathogenic, but the ability to produce YstA was detected in only 14 out of 96 examined strains. The fragments of ystA gene were also detected in all Y.enterocolitica strains isolated from human cases of diarrhea. Amplification of a fragment of the ymoA gene was detected in all the studied strains, both from humans and pigs, based on the presence of a 330 bp band. Thus no correlation was identified between the occurrence of the ymoA and ystA genes and the production of a specific type of enterotoxin.

6

Experimental immunization of animals against Yersinia enterocolitica infection

51%

Platt-Samoraj A. , Szweda W. , Siwicki A. K.

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nr 3

7

Role of goats in epizootiology and epidemiology of Q fever

51%

Platt-Samoraj A. , Ciecierski H. , Michalski M.

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tom 08

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nr 1

8

Przypadek zakazenia koz wirusem zapalenia stawow i mozgu koz w jednym ze stad w wojewodztwie warminsko-mazurskim

51%

Platt-Samoraj A. , Michalski M. , Sobiech P. , Mikulska-Skupien E.

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nr 09

802-804

9

Distribution of the ymoA and ystA genes and enterotoxins Yst production by Yersinia enterocolitica strains isolated from humans and pigs

51%

Szczerba-Turek A. , Szweda W. , Bancerz-Kisiel A. , Platt-Samoraj A.

Polish Journal of Veterinary Sciences

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2012

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nr 4

10

The influence of experimental Yersinia enterocolitica infection on the pregnancy course in sows - preliminary studies. I. Bacteriological examination

51%

Platt-Samoraj A. , Szweda W. , Procajlo Z.

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nr 3

EN

The aim of the study was to determine the influence of Yersinia enterocolitica (Y. enterocolitica) experimental infection on the carrying and shedding states of the microorganism by pregnant sows and on the bacteria occurrence in tissues of the infected animals and aborted or stillborn piglets. Twelve pregnant sows were divided into 4 groups and infected per os on 33 – group I (n=3), 54 – group II (n=3) and 89 – group III (n=3) day of pregnancy with the Y. enterocolitica strain isolated from the palatine tonsil of aborted swine fetus. The control group (n=3) remained uninfected. Rectal, oral and vaginal swabs from sows, placentas and the specimens of tissues from stillborn piglets were collected for bacteriological examination. Eight weeks after delivery, the sows were slaughtered and samples of internal organs were subjected to bacteriological examination. Pregnancy in all groups of sows took a normal course, and no cases of abortion were observed. Y. enterocolitica was isolated from oral, rectal and vaginal swabs of all infected sows. The number of stillborn piglets in the litters was highest in group III, where two macerated fetuses with putrefactive lesions were found. The bacteria were isolated from tissues of stillborn piglets in groups I and III, and only from placenta in group II. In rectal swabs of piglets in all groups, Y. enterocolitica was not isolated. The results of experimental infection of pregnant sows with Y. enterocolitica revealed that in animals infected in the last part of pregnancy, the microorganisms were isolated most frequently from vaginal, rectal and oral swabs of sows as well as from internal organ tissues of stillborn piglets.

11

Badania nad etiopatogeneza jersiniozy zakazonych doswiadczalnie swin

51%

Jakubczak A. , Platt-Samoraj A. , Siemionek J. , Anusz Z.

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nr 01

33-35

EN

The aim of the studies was to evaluate the carrier state of Yersinia enterocolitica and its effect on the immune response in experimentally infected pigs. The experiment was performed on 15 piglets aged eight weeks divided into three groups: I - piglets infected intravenously, II-animals infected intragastrically, III - a control group (non-infec- ted). In experimentally infected piglets the bacterial colonization caused by Y. enterocolitica was found in the intestines and tonsils and the carrier state lasted for 8 to 12 weeks. The infection brought about an increase in the level of specific antibodies (0:3). There was no correlation between the concentration of specific antibodies and the detectability of Y. enterocolitica in faeces of the piglets.

12

The influence of experimental Yersinia enterocolitica infection on the pregnancy course in sows - preliminary studies. II. Antibodies, C-reactive proteins and haptoglobin as an immunological response

51%

Platt-Samoraj A. , Szweda W. , Procajlo Z.

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nr 4

EN

The aim of the study was to determine the time of emergence and level of Y. enterocolitica antibodies in pregnant sows challenged orally with Y. enterocolitica in particular trimesters of pregnancy (groups I, II and III, respectively) and also the assignation of its influence on the CRP and Hp concentration in sera of pigs. Levels of antibodies measured by tube agglutination test increased slowly from 2 weeks post infection (wpi) and positive results were obtained not in all animals. In ELISA, in 2 weeks in all groups of infected animals high levels of antibodies against Y. enterocolitica were formed and lasted up to the end of the experiment. In newborn piglets in all groups, a significant decrease in antibody levels 6 weeks after birth was observed in both agglutination and ELISA tests. Concentrations of CRP as Hp in all groups of infected animals increased in 1 week post infection. Statistically significant differences (P ≤ 0.05) between CRP levels in groups I and II (46-fold and 44-fold) as well as III (29-fold) were revealed. In case of Hp, statistically significant differences between groups of animals in the first week post infection were not observed. Our findings indicate that Y. enterocolitica infection evoked strong and long-lasting immunological reaction in the form of specific antibodies production in all inoculated animals. The significant increase in CRP and moderate increase in Hp concentrations in the sera of pregnant sows also occurred. However, relationships between colostrums antibody levels in piglets’ sera and phase of pregnancy when the Y. enterocolitica infection happened in sows were not observed.

13

Wplyw zakazen Yersinia enterocolitica psow i kotow na wystepowanie jersiniozy u czlowieka

51%

Platt-Samoraj A. , Szweda W. , Siwicki A. K.

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nr 06

379-381

EN

An increase in Yersinia enterocolitica human infections has recently been noted. The numerous cases noted in other countries of this bacterium has begun to rival other widespread alimentary tract pathogens. Yersiniosis is a disease diagnosed mainly in humans, but symptoms of infection in numerous domestic and wild species of animals have been observed. Swine are the most important reservoir of human pathogenic Yersinia enterocolitica strains. However, there is evidence that pets can also be carriers of these strains, thus causing the disease in people. Most human pathogenic strains of Yersinia enterocolitica have been derived from puppies and kittens. These animals can be a source of infections, particularly during close contacts with children. Although dogs and cats are not the most important reservoir and source of Yersinia enterocolitica infections, this micro-organism should be included in differential diagnosis of enteritis, especially when its symptoms include bloody diarrhoea in young animals.

14

Effect of the coupled administration of Lydium-KLP or methisoprinol and respisure on the health status and prevention of mycoplasmal pneumonia of swine in large-scale commercial breeding

51%

Procajlo Z. , Szweda W. , Mikulska-Skupien E. , Platt-Samoraj A.

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2010

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tom 54

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nr 1

9-14

EN

The study was aimed on determining the clinical protective value of Lydium-KLP and Methisoprinol in the prevention of respiratory system diseases, as well as their effect on the health status of pigs in large-scale commercial breeding and on the improvement of in the effectiveness of specific immunoprophylaxis of mycoplasmal pneumonia of in swine (MPS). Piglets at the age of 7 d were divided into eight groups of 25-30 piglets each. On days 7 and 21 of life they were immunised with Respisure vaccine (group I) or administrated Lydium-KLP or Methisoprinol (groups IV and VII). In other groups, Lydium-KLP or Methisoprinol were given simultaneously (groups II and V) or 48 h before immunisation with Respisure (groups III and VI). Control piglets were administrated PBS (group C). Clinical observations as well as post-slaughter and animal husbandry analyses indicated that the simultaneous administration of Lydium-KLP or Methisoprinol with Respisure gives better results in comparison to immunisation- only with Respisure. The statistically-significant differences were found in the mean percentage of meatiness between groups receiving Methisoprinol with or 48 h before Respisure and control group, as well as in the point evaluation of lesions in the lungs between groups receiving Lydium-KLP with Respisure and Methisoprinol 48 h before Respisure and control group. The study demonstrated the beneficial effect of coupled administration of Lydium-KLP or Methisoprinol and Respisure on the improvement of health status and the productive performance of pigs, as well as on the effectiveness of specific immunoprophylaxis of MPS in large- scale commercial breeding. The most beneficial variants of coupled administration were the simultaneous application of Lydium- KLP and Respisure, and Methisoprinol application 48 h before immunisation with Respisure.

15

Zjadliwosc Yersinia enterocolitica oraz znaczenie biotypu 1A w patogenezie jersiniozy

51%

Platt-Samoraj A. , Bancerz-Kisiel A. , Szweda W.

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nr 10

1113-1115

EN

Yersinia enterocolitica is an extremely varied organism due to its biochemical, antigen and pathogenic properties. Six biotypes are distinguished within the species but, from the clinical point of view, only five of them: 1B, 2, 3, 4, and 5 are considered to be the cause of yersiniosis. The main criterion for the above division is pathogenicity, which correlates with presence of the virulent pYV plasmid. Microorganisms which lack this plasmid are considered to be a-virulent. However the pYV plasmid may easily be lost during the process of culturing or storage. Biotype 1A strains of Y. enterocolitica are often considered to be non-pathogenic primarily because they do not posses the pYV. These same strains of 1A biotype are able to cause diseases through unknown mechanisms that are independent of the virulent plasmid presence. Evaluating the pathogenicity of this biotype seems to be more complex. The increasing significance of 1A biotype and the possibility of loosing pYV in strains belonging to residual biotypes necessitate further studies which would clear up doubts relating to Y. enterocolitica pathogenicity.

16

Bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus)

45%

Szczerba-Turek A. , Socha P. , Szweda W. , Bancerz-Kisiel A. , Platt-Samoraj A.

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nr 2

17

Activity of T and B lymphocytes and blood phagocytes in pigs immunomodulated with bioimmuno and/or immunised with respisure one vaccine against mycoplasmal pneumonia of swine

45%

Procajlo Z. , Szweda W. , Siwicki A. , Platt-Samoraj A. , Mikulska-Skupien E.

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nr 4

EN

The purpose of the studies was to determine the proliferation activity of T and B lymphocytes as well as the metabolic and potential killing activity of blood phagocytes in pigs after the immunomodulation with the Bioimmuno preparation and/or immunisation with the 'Respisure One' vaccine against mycoplasmal pneumonia of swine. The studies were performed on piglets at the age of 4 weeks, divided into four groups of seven animals each. The biopreparations were administered according to the following pattern: Bioimmuno (1 kg/50 kg of feedstuff) for 48 h before vaccination with Respisure One (2 ml/animal i.m.) on the 28th d of life (group I), Bioimmuno (1 kg/50 kg of feedstuff) on days 26 and 27 of life (48 h before vaccination with Respisure One of groups I and III) (group II), Respisure One on the 28th d of life (group III) and PBS (2 ml/animal i.m.) simultaneously with vaccination of groups I and III (group C - control). The proliferation activity of T and B lymphocytes (MTT test) and the metabolic and potential killing activity of blood phagocytes (RBA and PKA tests) were determined in whole blood. Statistically significantly higher (P<0.05) proliferation activity of Con A-stimulated T lymphocytes as well as LPS-stimulated B lymphocytes were found in the stimulated and immunised group I and the immunised group III in comparison with the stimulated group II and group C. Higher metabolic and potential killing activities of the phagocytes were also detected in all experimental groups compared with the control one. The studies have demonstrated that the Bioimmuno immunomodulator and/or the Respisure One vaccine stimulate the proliferative response of T and B lymphocytes, as well as they increase the metabolic and potential killing activity of blood phagocytes, contributing to the improvement in the immune system functioning and to the protection of the swine organism against the infections of the respiratory system.

18

Application of multiplex PCR for the evaluation of the occurrence of ail, ystA, and ystB genes in Yersinia enterocolitica strains isolated from wild boars (Sus scrofa)

45%

Barcerz-Kisiel A. , Szczerba-Turek A. , Platt-Samoraj A. , Socha P. , Szweda W.

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nr 3

EN

The study aimed at the evaluation of the occurrence of genes directly connected with pathogenicity in Yersinia enterocolitica (Y. enterocolitica) strains isolated from wild boars, obtained in the 2007/2008 hunting season in the North-East territory of Poland. Multiplex PCR, used for αil, ystA, and ystB gene detection, was optimised in order to determine the existence of the genes in one reaction. Forty-six palatine tonsils, gained from 46 wild boars of various ages, were preliminary examined from the bacteriological, sero- and biotyping points of view; then bacterial DNA was isolated and multiplex PCR was performed. The presence of the product of volume corresponding to the ystB gene fragment was found in two cases, which constituted 4.35% of the examined samples. The ail and ystA genes were not detected in any of the tested samples. The molecularly confirmed existence of Y. enterocolitica in wild boar palatine tonsils, indicates the carrier state and possibly shedding of the microorganism into the environment, and suggests that wild boars, likewise pigs to a lesser extent, may constitute a reservoir of the bacteria and a potential source of infection for the man.

19

Dynamics of variations of acute phase proteins in pigs fed Bioimmuno and/or immunised with Respisure One vaccine against mycoplasmal pneumonia

45%

Procajlo Z. , Szweda W. , Siwicki A. , Platt-Samoraj A. , Mikulska-Skupien E.

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2011

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tom 55

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nr 1

EN

The purpose of the study was to determine the influence of the Bioimmuno administration in feed and/or immunisation with the 'Respisure' One vaccine against mycoplasmal pneumonia of swine on the development of non-specific immune response expressed by the level of acute phase proteins (APP). The study was performed on 28 piglets at the age of 4 weeks, divided into four equal groups. The biopreparations were administered according to the following pattern: group I - Bioimmuno (1 kg/50 kg of feedstuff) for 48 h and then vaccination with Respisure One (2 ml/animal i.m.), group II - Bioimmuno ( 1 kg/50 kg of feedstuff) for 48 h, group III - Respisure One (2 ml/animal i.m.), and group C (control) - PBS (2 ml/animal i.m.). Serum concentration of APP: C- reactive protein, haptoglobin, and ceruloplasmin were determined on days 0, 3, 7, 14, and 21 after immunomodulation and/or immunisation. The statistically significantly lower (P<0.05) levels of all determined APP in the experimental groups in comparison with the control group were demonstrated. This proves the protective value of the biopreparations applied in specific patterns, through their beneficial influence on the decrease in consequences of the respiratory system infections in pigs.

20

Sytuacja epizootyczna choroby Aujeszkyego w woj.olsztynskim w latach 1992-1997

45%

Szweda W. , Jedryczko R. , Pirus T. , Siemionek J. , Platt-Samoraj A.

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nr 03

176-180