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2 Acta Neurobiologiae Experimentalis

2 2010

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Combined estradiol and lithium increase ER-α mRNA in embryonic C57BL/6J primary hippocampal cultures

100%

Valdes J. J. , Weeks O. I.

Acta Neurobiologiae Experimentalis

2010

tom 70

nr 3

Estrogen replacement therapy (ERT) is commonly prescribed during menopause. Post-menopausal women also tend to suffer from bipolar disorders and as a result are prescribed mood stabilizers – in addition to ERT. There is a paucity of data on how combined hormones and mood stabilizers interact in regulating gene expression that led us to hypothesize that in primary cultures of mixed brain cells predominated by glia, combined 17β-estradiol (E2) and lithium chloride (LiCl) (E2/LiCl) will alter estrogen receptor-alpha (ER-α) mRNA expression. We quantified mRNA expression of ER-α using the cDNA of treated primary cultures of mixed brain cells from a previous study. Our results indicate that hippocampal cultures predominated by glia increase in ER-α mRNA expression when treated for 48 h with combined E2/LiCl. Our findings may encourage further investigation on the molecular mechanisms involved in combined estrogen and lithium treatment.

Lithium enhances cortical mRNA expression in ovariectomized C57BL/6J mice

80%

Valdes J. J. , Ramirez F. M. , Juarez B. , Weeks O. I.

Acta Neurobiologiae Experimentalis

2010

tom 70

nr 3

The hippocampus and cortex of the mammalian brain are regions involved in learning and long-term memory. Estrogen and lithium affect similar learning and memory molecular processes. We hypothesized that in ovariectomized mice lithium treatment will enhance genetic factors in the brain that are involved in neuroprotection, learning and memory. Our study used bilaterally ovariectomized (bOVX) C57BL/6J mice treated for one month with 14.2 mM LiCl in their drinking water. Results indicate that LiCl-treated bOVX mice show enhanced cortical increases in mRNA expression of ER-α, NR1, Bcl-2, BDNF, and CaMkII-α; hippocampal mRNA showed no changes. Our results indicate that in bOVX C57BL/6J mice, lithium enhances the expression of specific cortical genes coded for proteins involved in learning, memory and neuroprotection.