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3 Journal of Applied Genetics

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Chromosomes of Lupinus hispanicus subsp. hispanicus Boiss. et Reut., L. luteus L. and their hybrids

100%

Naganowska B. , Ladon D.

nr 3

167-170

Cytological observations of mitotic chromosomes were performed for two species and interspecific hybrid lines: Lupinus hispanicus subsp. hispanicus (line Badajoz 2, cat. no. Wt 96383), L. luteus cv. Topaz (cat. no. Wt 98006), L. ? hispanicoluteus (cat. no. Wt 98301 and Wt 98302). It was found that L. hispanicus, L. luteus and L. ? hispanicoluteus have the same chromosome number 2n = 52. Chromosome morphology is, generally, similar. Centromeres are located mostly in median or submedian region. Chromosome differentiation appears to be very poor, except the first (the longest) pair which is easily discernible in both parental species and in the hybrid.

1;25 centric fusion recognition in the cattle chromosome by fluorescence in situ hybridization

80%

Ladon D. , Solinas-Toldo S. , Fries R. , Stranzinger G.

nr 1

101-104

Fluorescence in situ hybridization (FISH) experiments with specific probes for chromosome 29 and 25 were carried out on a Brown Swiss bull, previously diagnosed as a carrier of 1;29 centric fusion.The hybridization of the chromosome 29-specific probe (BMC 4216-already located on 29q13), produced signals on two small acrocentrics, but not the translocated chromosome.The signals appeared on the translocated chromosome and on a single chromosome 25 after hybridization of the chromosome-specific probe (BMC 3224 -previously located on 25q24).According to the actual nomenclature, the analysed aberration is a robertsonian translocation involving chromosomes 1 and 25.

Monitoring of cellular chimerism in patients after sex-mismatched bone marrow transplantation: technical report

80%

Jolkowska J. , Ladon D. , Wachowiak J. , Witt M.

nr 3

209-212

Numerous hematological diseases, in particular leukemias, can be treated successfully with allogeneic bone marrow transplantation (allo-BMT). Highly polymorphic microsatellite markers and X, Y-chromosome-specific sequences provide useful genetic markers for detection of complete or mixed chimerism in patients after allo-BMT. Chimerism can be monitored successfully using polymerase chain reaction technique (PCR) and cytogenetic analysis, especially fluorescent in situ hybridization (FISH). It is still unclear whether individuals with mixed chimerism after bone marrow transplantation have an increased risk of developing leukemic relapse or graft rejection. Molecular study of cellular chimerism can also be used for quantitative assessment of the amount of donor's cells in a recipient after bone marrow transplantation and for monitoring of minimal residual disease (MRD) or disease relapse. We report application of three different DNA-typing techniques: automated DNA sizing technology, fluorescent in situ hybridization and also Y-specific DNA probing for analysis of post-BMT chimerism in a case of sex-mismatched bone marrow transplantation. Key words: allo-BMT, chimerism, FISH, PCR.