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In vitro interaction between fumonisin B1 and the intestinal microflora of pigs

100%

Dang H. A. , Zsolnai A. , Kovacs M. , Bors I. , Bonai A. , Bota B. , Szabo=Fodor J.

Polish Journal of Microbiology

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2017

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tom 66

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nr 2

EN

The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B₁ (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B₁) and control 1 groups (buffer + chyme) were observed in both methods. Fumonisin B₁ and hydrolysed fumonisin B₁ concentration were analysed by liquid chromatograghy – mass spectrometry. There was no significant difference in FB₁ concentration between the experimental and the control 2 group (buffer and fumonisin B₁) at 0 h incubation, 5.185 ± 0.174 μg/ml compared with 6.433 ± 0.076 μg/ml. Fumonisin B₁ concentration in the experimental group was reduced to 4.080 ± 0.065 μg/ml at 24 h and to 2.747 ± 0.548 μg/ml at 48 h incubation and was significantly less than that of in the control group. Hydrolysed fumonisin B₁ was detected after 24 h incubation (0.012 ± 0 μg/ml). At 48 h incubation time, hydrolysed fumonisin B₁ concentration was doubled to 0.024 ± 0.004 μg/ml. These results indicate that fumonisin B₁ can be metabolised by caecal microbiota in pigs though the number of studied bacteria did not change.

2

Comparison of different Hungarian Grey herds as based on microsatellite analysis

84%

Zsolnai A. , Kovacs A. , Anton I. , Ratky J. , Brussow K. P. , Jozsa C. , Ban B. , Gyurman A.

Animal Science Papers and Reports

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2014

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tom 32

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nr 2

EN

The present study aimed at assessing the genetic position of the Hungarian Gray population. Hungarian Grey cattle kept at different farms in Hungary have been sampled (34 herds, n=3,187 in the period of 2009-2011) to investigate their genetic relationship based on analysis of allelic variation at eleven microsatellite loci. The mean observed heterozygosities per herd were above a moderate degree (0.60-0.80). Calculation of pairwise genetic distances and analysis of the history of herds revealed that among the most closely related herds we can find those, which are the core of the current Hungarian Gray population. The results of the population differentiation showed that all Hungarian Gray herds were significantly different from each other. In most cases (22 herds) FST values were within a range of low degree of genetic differentiation (0.003-0.050), while the remaining 12 herds differed from the central population by FST values of 0.060-0.119. Principal coordinate analysis, assignment tests and dendrograms all suggest that there are mainly two different groups among Hungarian Grey herds. Structure analysis has yielded K=3 as the most probable number of clusters. Based on the private allelic richness, genetic distance and FIS values identified were 12 herds where more attention should be paid by the management to avoid genetic drift and to preserve genetic diversity. The results presented could also contribute to the proper selection of animals for further whole genome scan studies of Hungarian Grey.

3

Physiological effects of whey- and milk-based probiotic yogurt in rats

84%

Szabo-Fodor J. , Bonai A. , Bota B. , Egyed L. S. , Lakatos F. , Papai G. , Zsolnai A. , Glavits R. , Horvatovich K. , Kovacs M.

Polish Journal of Microbiology

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2017

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tom 66

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nr 4

EN

In an in vitro experiment commercially available probiotic products were tested for the survival of bacteria under conditions of simulated human digestion either when used alone or mixed into yogurt. In the in vivo experiment the effects of feeding a whey- and milk-based yogurt prepared with the probiotic strain showing adequate survival in the in vitro experiment, was measured on body weight, feed consumption and immune response of rats (IgG and IgA level after immunisation), on the composition and volatile fatty acid production of the intestinal microbiota and on the structure of intestinal villi. The Lactobacillus acidophilus (LA-15) strain had inadequate surviving ability in rats. Bifidobacterium animalis ssp. lactis (BB-12) improved the composition of the intestinal microflora, whereas whey-containing product had a mild immunostimulating effect and exerted a favourable influence on the morphology of intestinal villi. The consumption of yogurts increased the depth of crypts in the ileum, which resulted in enhanced secretion and thus softer faeces.

4

Lethal Angiostrongylus vasorum infection in a Hungarian dog

84%

Benda T. , Csivincsik A. , Nemes C. , Turbok J. , Zsolnai A. , Simonyai E. , Majoros G. , Nagy G.

Acta Parasitologica

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2017

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tom 62

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nr 1