Wyniki wyszukiwania - Biblioteka Nauki

1

Krzemowy skarb

100%

Bienkowski M.

Wiedza i Życie

|

2013

|

nr 03

2

Ewolucja "cegły"

100%

Bienkowski M.

Wiedza i Życie

|

2013

|

nr 06

3

Effect of basic ingredients of a salt-including antipyren agenton timber fire protection

63%

Bienkowski M. , Cyrankowski M.

|

tom 56

4

Effect of epoxy lacquer for increasing the durability of timber fire-protection with salt-containing agents

63%

Bienkowski M. , Cyrankowski M.

|

tom 56

5

Obliczenia na kwantach

63%

Bienkowski M.

Wiedza i Życie

|

2014

|

nr 03

6

Czas atramentowych "plujek"

63%

Bienkowski M.

Wiedza i Życie

|

2008

|

nr 04

7

The influence of selected chlorine compounds on the effectiveness of fireprotection of timber

51%

Bienkowski M. , Cyrankowski M. , Osipiuk J.

Annals of Warsaw Agricultural University. Forestry and Wood Technology

|

2006

|

tom 58

8

Chlorine, sulphur and potassium compounds as antipyrins used to protect pine wood

51%

Bienkowski M. , Osipiuk J. , Cyrankowski M.

|

2007

|

tom 61

9

Ocena skutecznosci dezynfekcji pola operacyjnego i jej wplywu na czestosc zakazenia miejsca operacyjnego u chorych poddawanych cholecystektomii laparoskopowej

51%

Bienkowski M. , Gospodarek E. , Olejarz A. , Deptula A.

Medycyna Doświadczalna i Mikrobiologia

|

2007

|

tom 59

|

nr 2

183-191

10

The research on influence of silicone agents on increase in the durability of wood fire-protection with the use of saline antipyrene

51%

Cyrankowski M. , Bienkowski M. , Osipiuk J.

Annals of Warsaw Agricultural University. Forestry and Wood Technology

|

2006

|

tom 58

11

Impact of sodium compounds on pine wood fire protection

51%

Bienkowski M. , Cyrankowski M. , Osipiuk J.

|

2007

|

tom 61

12

Research on the properties of selected timber fire-protective chemical compounds with the usage of combustion tube

51%

Bienkowski M. , Cyrankowski M. , Osipiuk J.

Annals of Warsaw Agricultural University. Forestry and Wood Technology

|

2006

|

tom 58

13

Influence of non-washable impregnator on increase in the durability of wood fire-protecion with the use of saline antipyrene

51%

Cyrankowski M. , Bienkowski M. , Osipiuk J.

Annals of Warsaw Agricultural University. Forestry and Wood Technology

|

2006

|

tom 58

14

Laserem po papierze

44%

Bienkowski M.

Wiedza i Życie

|

2008

|

nr 05

15

In vitro cultured 3D aggregates of glioblastoma cells preserve in vivo observed phenotype and genotype of this tumour

26%

Witusik-Perkowska M. , Bienkowski M. , Jurga M. , Stoczynska-Fidelus E. , Hulas-Bigoszewska K. , Roci I. , McGuckin C. P. , Banaszyk M. , Wolanczyk M. , Sikorska B. , Liberski P. P. , Rieske P.

|

nr 1

EN

Surprisingly in vitro conditions allowing to culture glioblastoma cells presenting EGFR amplification were not known until now. Our evaluation of EGFR amplification status in glioblastoma (GBM) culture demonstrated that this anomaly was preserved for months in spheroids (aggregated glioblastoma cells) at a level comparable to the earliest passage of cell culture. In contrast, and in accordance with already published data we detected it as completely lost in the adherent culture. Apparently glioblastoma cells presenting EGFR amplification become apoptotic in the regular cell culture conditions. In addition discrepant expression of SOX2 and multilineage phenotype recognized as a markers of neural progenitors was observed in monolayer and 3D culture. Moreover, our analyses showed a decreased invasion potential of adherent GBM loosing EGFR amplification, and spheroids maintaining EGFR amplification. In conclusion, our findings confirm that GBM-derived spheroids seem to be a promising tool to preserve original molecular features of the tumor in vitro, with a special emphasis on EGFR gene aberrations, including EGFRvIII, regarded as novel therapeutic target. Our last unpublished and preliminary data suggest that mechanism responsible for in vitro death of adherent glioblastoma cells showing EGFR amplification seems to be linked to the artificial in vitro cell-cell interaction rather than to the lack of proper autocrine effects. Those suggestion came from analysis of glioblastoma cells in artificial brain tissues system and analysis of aggregated glioblastoma cells only. The present study was supported by the Ministry of Science and Higher Education, Poland, grant No. N N401047337 and Foundation for Polish Science(PARENT-BRIDGE Programme - support for women, No.POMOST_C/15).