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1

Badania hydrofobowosci szczepow Proteus vulgaris oraz zdolnosci szczepow Proteus vulgaris i Proteus penneri do penetracji komorek nablonka pecherza moczowego HCV T-29

100%

Bartodziejska B. , Blaszczyk A. , Wykrota M. , Kwil I. , Babicka D. , Rozalski A.

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nr 4

335-345

PL

Zbadano hydrofobowe oraz inwazyjne właściwości szczepów P. vulgaris należących do różnych grup serologicznych, wytwarząjących hemolizynę HlyA, a także szczepów P. penneri wytwarząjących różnego typu fimbrie. Stwierdzono, że rodzaj wytwarzanych fimbrii nie wpływa na inwazyjne zdolności badanych szczepów. Bakterie wytwarząjące hemolizyny działały cytotoksycznie wobec komórek HCV T-29.

EN

Proteus bacilli play a particularly important role in urinary tract infections (UTI). Fimbriae and adherence ability and hemolysins production (HpmA, HlyA) are one of the factors of pathogenicity of these bacteria. In this paper we describe the invasion of HCV T-29 transitional bladder urothelial cells carcinoma strains of P. penneri, as well as P. vulgaris strains belonging to different serogroups. The cytotoxic effect was observed at 8 hour of incubation of the tested cells with P. vulgaris O21 and the same effect (complete lysis) at 6 hours by P. vulgaris O4 (this strain manifests maximal activity in the production of HlyA hemolysin). P. penneri strains, produce different types of fimbriae, expressed similar bacterial invasiveness. The hydrophobic properties of 25 P. vulgaris strains were also tested and only 3 strains occur to have hydrophobic cell surface.

2

Przeciw gryzoniom w oborach, stajniach i chlweniach

99%

Babicka D. , Grubisz M.

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nr 11

3

Struktura chemiczna, swoistość antygenowa i znaczenie w chorobotwórczości lipopolisacharydu (LPS, endotoksyna) na przykładzie bakterii Proteus vulgaris

84%

Różalski A. , Torzewska A. , Bartodziejska B. , Babicka D. , Kwil I. , Perepelov A. V. , Kondakova A. N. , Senchenkova S. N. , Knirel Y. , Vinogradov E. V.

Wiadomości Chemiczne

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2002

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tom [Z] 56, 7-8

585-604

EN

Gram-negative bacteria of the genus Proteus from the family Enterobacteriaceae are opportunistic pathogens, which cause mainly wounds and urinary tract infections (UTI), the latter leading to severe complications, such as acute or chronic pyelonephrithis and formation of bladder and kidney stones. Virulence factors and properties of Proteus sp. mediating infectious process are swarming phenomenon, adherence due to the fimbriae or glycocalyx, flagella, invasiveness, urease, amino acids deaminases, proteases, hemolysins, capsular polysaccharide (CPS), and lipopolysaccharide (LPS). LPS is an integral component of cell wall of bacteria. It also represents the endotoxin which, after being released from bacterial cells, causes a broad spectrum of pathological effects leading in severe cases to the septic shock. Lipopolysaccharide consists of three parts - O- specific chain (O-antigen), core and lipid A; all of them have been studied in Proteus LPS. It has been documented that Proteus is an antigenically heterogeneous genus, principally because of structural differences in its O-specific polysaccharide chain of LPS. The serological classification of P. mirabilis and P. vulgaris shares 60 serogroups : 22 described for P. vulgaris, 33 characteristic for P. mirabilis and 5 common for both P. mirabilis and P. vulgaris. Serological classification of Proteus penneri still remains to be completed. Proteus O-antigens are branched or linear polysaccharides, built up of oligosaccharide repeating units, varying from a trisaccharide to a hexasaccharide. Acidic O-specific polysaccharides represent the majority of Proteus O-antigens; it was found that 80% of Proteus O-antigens were acidic. Uronic acids and amino sugars usually determine the serological specificity of Proteus O-antigens. Amino sugars in Proteus O-antigens are usually N-acetylated. In many O-antigens, sugars constituents carry an O-acetyl groups. Hexuronic acids either have free carboxyl group or are amidated with the a-amino group of amino acids - lysine, serine, alanine or threonine. Chemical and serological studies have been undertaken with the aim to understand on the molecular level the immunospecificity of Proteus LPS and its potential role during infection of bacteria. The O-antigens and O-antisera against Proteus with defined epitope specificity can be used for serodiagnosis and epidemiological studies. It was found that O-specific polysaccharide Proteus bacteria is involved in creation of glycocalyx which allows bacteria to grow in microcolony or in biofilm. Biofilm protects bacteria against action of antimicrobial agents and leukocytes, and it is also a organic gel-like surrounding contributing to stone formation. LPS from the S form of bacteria, containing all three regions also contributes to their resistance against bactericidal action of serum. The present review is mainly focused on the structure, specificity and biological function of Proteus vulgaris LPS.

4

Przeciwko gryzoniom

83%

Babicka D.

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1986

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nr 12