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Mitochondrial DNA sequence from an extinct population of European vendace, Coregonus albula L.

100%

Brzuzan P. , Ciesielski S.

nr 3-4

151-154

Nucleotide sequence of the mitochondrial DNA control region was determined for the formalin-preserved specimen of the individual European vendace, Coregonus albula, belonging to an extinct population. The specimen has been preserved and stored in formalin for 39 years. A comparison with two previously determined sequences obtained for specimens from other populations of vendace revealed both nucleotide substitutions and site heteroplasmy. The ability to sequence DNA from formalin-preserved museum specimens of rare or extinct taxa provides another sampling strategy for phylogenetic studies on fish.

Accumulation of polyhydroxyalkanoates (PHAs) in activated sludge under nitrogen limitation conditions. Part II. Estimation of bacterial diversity in activated sludge and analysis of a type of accumulated polyhydroxyalkanoates

80%

Klimiuk E. , Ciesielski S. , Pokoj T. , Rogowski J.

nr 4

214-226

Community diversity of the activated sludge and the type of accumulated PHAs were investigated in this study. Fed-batch culture was carried out using medium culture containing sodium acetate as a carbon source. A factor triggering PHAs synthesis was a lack of nitrogen. The medium culture containing ammonium was periodically fed into bacterial culture for 6 hours each day of the experiment. Genetic analysis of the bacterial community was performed using 16S and 23S rRNA Intergeneric Spacer Region. The obtained results showed that the dominant belonged to the Betaproteobacteria class. In this study, the Time of flight - Secondary Ion Mass Spectrometry was used. This method revealed that the cells of the microorganisms contained mainly monomer units of 3-hydroxybutryric acid. The content of P(3HB) in dry weight accounted for 51% between 72nd and 120th hour of the experiment.