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Effects of inhibitor of Src kinases, SU6656, on differentiation of megakaryocytic progenitors and activity of alpha1,6-fucosyltransferase

100%

Kaminska J. , Klimczak-Jajor E. , Skierski J. , Bany-Laszewicz U.

Acta Biochimica Polonica

2008

tom 55

nr 3

499-506

α1,6-fucosyltransferase (FUT8) attaches fucose residues viaan α1,6 linkage to the innermost N-acetylglucosamine residue of N-linked glycans. Glycans with this type of structure are present in GpIIb/GpIIIa complex (CD41a) which is present on megakaryocytes (Mks) and platelets. CD41a is the earliest marker of megakaryocytopoiesis. The aim of this study was to analyse the morphology, phenotype, ploidy level and activity of FUT8 during induced differentiation/maturation of Mk progenitor cells in ex vivoculture. We used SU6656, a selective inhibitor of Src tyrosine kinases, as differentiation-inducing agent for Mks. The addition of SU6656 to the culture system of megakaryocytic progenitors from cord blood CD34+cells and Meg-01 cell line induced their maturation towards later stages of Mk differentiation with increased activity of FUT8. We suggest FUT8 as a candidate for an early marker of differentiation and possibly of the ploidy level of Mks. We confirm a special status of FUT8 in megakaryocytopoiesis.

Kinazy Src w procesie dojrzewania progenitorów megakariocytów

100%

Kaminska J. , Klimczak-Jajor E. , Bany-Laszewicz U.

Postępy Biochemii

2008

tom 54

nr 4

The activity of alpha 1,6-fucosyltransferase during human megakaryocytic differentiation

100%

Bany-Laszewicz U. , Kaminska J. , Klimczak-Jajor E. , Koscielak J.

Cellular and Molecular Biology Letters

2004

tom 09

nr 1

α1,6-Fucosyltransferase (6FucT, E.C. 2.4.1.68) is one of the enzymes involved in the synthesis of N-linked glycans of the GpIIb/IIIa complex (CD41a) which is present on megakaryocytes (MKs) and platelets. In this study, we examined 6FucT activity in ex vivo cultures of immunoselected cord blood CD34+ cells grown in a medium promoting megakaryocytopoiesis. Our results show that the activity of 6FucT increased ahead of, and thereafter concomitantly with, cells expressing the CD41a antigen. When the CD41a+ subpopulation of cells was immunoselected (using anti-CD61 i.e. anti-GpIIIa antibodies), its 6FucT activity increased proportionally to the yield of CD61+ cells. Taking into account the heavy load of 6FucT in platelets and megakaryocytes, we regard this enzyme as a candidate for the earliest marker of MK-commitment in cultured hematopoietic stem cells. Such a marker should allow an earlier detection and earlier transplantation of patients’ own, ex vivo expanded, Mk progenitors.

Ocena dojrzewania komorek progenitorowych megakariocytow w hodowli in vitro komorek macierzystych hematoporezy

88%

Bany-Laszewicz U. , Klimczak-Jajor E. , Palynyczko G. , Koscielak J. , Kaminska J.

Postępy Biologii Komórki. Suplement

2003

tom 21

61-71

Src kinases inhibitor, SU6656, induces differentiation and maturation of megakaryocyte progenitor cells

75%

Kaminska J. , Bany-Laszewicz U. , Klimczak-Jajor E. , Skierski J. , Zdebska E. , Koscielak J.

Acta Biochimica Polonica

2007

tom 54

nr Supplement 4

Pierwszy przypadek nieimmunologicznego obrzęku płodu (HbBart’s) w wyniku alfa talasemii zdiagnozowany w Polsce

63%

Skulimowska J. , Turowski P. , Klimczak-Jajor E. , Debska M. , Pyl H. , Guz K. , Uhrynowska M. , Brojer E.

nr 1A

Identyfikacja mutacji w genach w klastrze alfa globiny z zastosowaniem gap-PCR, MLPA i sekwencjonowania

63%

Klimczak-Jajor E. , Skulomowska J. , Turowski P. , Guz K. , Uhrynowska M. , Mendek-Czajkowska E. , Ejduk A. , Kopec I. , Adamowicz-Salach A. , Brojer E.

nr 1A