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Straight and branched (ω-1)-hydroxylated very long chain fatty acids are components of Bradyrhizobium lipid A

100%

Choma A. , Komaniecka I.

tom 58

nr 1

Lipopolysaccharides of seven Bradyrhizobium strains and three whole-cell fatty acid preparations from bacteria isolated from nodules of Sarothamnus scoparius (Common Broom) were studied for the presence of very long chain (ω-1)-hydroxy fatty acids. Several such fatty acids were identified. Among them, straight-chain as well as mono- and dimethyl branched acids with chains in the range from 26 to 34 carbon atoms were found. Pyrrolidides and 4,4-dimethyloxazoline derivatives were used to determine the branching position. Carbons at the (ω-10) and/or (ω-11) positions in alkyl chains were points of attachment of methyl groups. These data complete the structure of bradyrhizobial lipid A with important details. The obtained results can be applied in the chemotaxonomy of Bradyrhizobium.

Kwasy tłuszczowe błon erytrocytarnych pacjentów z przewlekłą niewydolnością nerek (PNN)

80%

Kimak E. , Komaniecka I. , Solski J.

tom 37

nr 2

Biological activity of Nod factors

61%

Kidaj D. , Krysa M. , Susniak K. , Matys J. , Komaniecka I. , Sroka-Bartnicka A.

tom 67

nr 4

Characterisation of the periplasmic beta-glucans isolated from Azorhizobium caulinodans HAMBI 21

61%

Komaniecka I. , Choma A. , Urbanik-Sypniewska T. , Russa R. , Turska-Szewczuk A. , Koltun J.

nr Supplement 1

Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance

61%

Komaniecka I. , Zamlynska K. , Zan R. , Staszczak M. , Pawelec J. , Seta I. , Choma A.

nr 3

Fruiting bodies of Hericium erinaceus (Bull.) Pers. - a new source of water-insoluble (1→3)-alfa-d-glucan

51%

Wiater A. , Choma A. , Komaniecka I. , Pleszczynska M. , Siwulski M. , Polak p. , Janusz G. , Szczodrak J.

nr 3

A water-insoluble polysaccharide (WIP) was isolated from the fruiting bodies of Hericium erinaceus HE01 by an alkaline solution with the yield of 5%. Structural and compositional analyses by total acid hydrolysis, methylation analysis, FT-IR, FT-Raman, and ¹H NMR spectroscopy as well as other instrumental techniques showed predominantly glucose linked by α-glycosidic bonds and small amounts of mannose, xylose, rhamnose, galactose, and ribose. The methylation analysis showed that (1→3)-linked Glcp is the major constituent (70.8%) of the polymer, while the 3,4 substituted d-Glcp represents the main branching residue of the glucan. The presence of (1→3)-α-d-glucan in the hyphae of H. erinaceus was additionally confirmed by the use of specific fluorophore-labeled antibodies.