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EN
Genome-wide gene expression profiling was conducted by Solexa sequencing in order to gain insight into the transcriptome dynamics that are associated with salt stress of cotton seedlings. A total of 145,794 and 138,518 clean tags were generated from the control and salinity libraries, respectively. Of these, 75,500 (51.8%) and 72,077 (52.0%) tags were matched to the reference genes. The most differentially regulated tags with a log2ratio >2 or >-2 (P <0.001) were analyzed further, representing 125 up- and 171 down-regulated genes except for unknown transcripts, which were classified into ten functional categories. The most enriched categories were those of metabolism, signaling pathway, environmental response and transcription. Many genes or biological pathways were found to be commonly shared between salt and other abiotic stresses in plants such as genes participating in environmental response, ABA signaling JA signaling, etc. Furthermore, the expression patterns of 12 genes were assessed by quantitative real-time PCR, and the results obtained showed general agreement with the Solexa data. Further analysis indicated the important roles of selected genes in salt tolerance by comparison with the mRNA levels in salt-tolerant cotton cultivar ZM3 with that in salt-sensitive cultivar LM6. Overall, we reveal the complex changes at the transcriptional level during salt stress of cotton seedlings and provide useful starting points for more in-depth analyses of cotton’s salt tolerance.
EN
Human gait motion analysis was one useful method for lower limb prosthesis study. The most often measured parameters were plantar pressure, kinetic and kinematic parameters. It was indispensable for prosthetic knee design and performance assessment. The aim of this study was to analysis the plantar pressure in traumatic above-knee amputee equipped with a developed microprocessor-controlled prosthetic knee. Methods: The maximum force of forefoot and rearfoot, the average vertical reaction force and pressure and the centre of pressure (COP) offset trajectories of ten above-knee amputees under different walking speeds were obtained. Results: Both forefoot and rearfoot force were bigger in intact leg than prosthetic leg. As the speed increased, the pressure increased in both sides. Forefoot bore more pressure than rearfoot in both legs. The average vertical pressure and force both increased along with the increase of speed. The force and pressure of intact side were always bigger than the prosthetic side. The trend of COP and gait line of the prosthetic and intact side had no significant difference. The length of the gait line of prosthetic side was greater than the intact side. Conclusions: The results of this study exhibited reduced plantar pressure in the prosthetic side. The typical butterfly diagrams were produced during different walking speeds. It indicated that the stability of the microprocessor-controlled prosthetic knee could be guaranteed.
3
Content available remote Synthesis and characterization of jar-like ZnO structures
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EN
A new jar-like ZnO structure was synthesized by heating a mixture of Zn and InI3 powder with a weight ratio of 4:1 dispersed on Si wafer at 450 °C in air. The diameter of the jar was of the order of 15 ?m and the length of 20 ?m. The formation of a molten InI3 drop, coating the drop with Zn powders, oxidation of these Zn grains, decomposition of InI3 to In and I vapors and the subsequent release of these vapors from the structure are considered important steps in the formation of the observed structures. The necessary elements in forming such structures are analyzed, which can be used as a guide in the design of experiments to synthesize similar structures for different purposes. Such structures are predicted to be able to soak large amount of liquid and release it at low rate, which is a desired property in some applications.
EN
The effects of different concentrations (10-5 M, 10-4 M. 10-3 M) of Cu2+ on growth, antioxidant enzyme activity and malondialdehyde (MDA) content were investigated in hydroponically grown Allium sativum L. The results indicated that the growth of garlic seedlings was not inhibited under treatment with 10-5 M Cu2+. Garlic seedlings exposed to 10-4 M and 10-3 M Cu2+ exhibited significant growth reduction. With increasing Cu2+ concentration and treatment time, superoxide dismutase (SOD) activity increased in leaves and roots, and peroxidase (POD) activity increased in leaves. In roots of plants exposed to 10-4 M and 10-3 M Cu2+, POD activity increased within 9 d and then dropped, but was still higher than in the control at the end of the experiment. Catalase (CAT) activity increased in seedlings grown at 10-5 M and l0-4 M, whereas a highly toxic level of Cu2+ (10-3 M) markedly inhibited CAT activity. SOD and POD activity were higher in roots than in leaves, whereas CAT activity was higher in leaves than in roots under both control and Cu2+ treatments. There was no obvious effect on MDA content in the seedlings treated with 10-5 M Cu2+; at 10-4 M and 10-3 M Cu2+ it increased. The mechanisms of Cu2+ toxicity and Cu2+ tolerance in garlic are briefly discussed.
EN
The effects of different concentrations of Al (10 μM, 50 μM, 100 μM) on nucleoli in root tip cells, root growth, antioxidant enzyme activity and malondialdehyde (MDA) content were investigated in hydroponically grown Vicia faba L. Aluminum significantly inhibited root growth of V. faba treated with 50 μM and 100 μM Al. In the nucleolus in root tip cells, some particulates containing argyrophilic proteins were extruded from the nucleus into the cytoplasm, and some were scattered in the nucleus after Al stress. Superoxide dismutase (SOD) activity in leaves and roots exposed to different concentrations of Al was mostly higher than in the control. Seedlings exposed to 100 μM Al showed significantly higher peroxidase (POD) activity in roots than in the control. POD activity increased much more in roots than in leaves. Catalase (CAT) activity was lower in roots than in leaves. Malondialdehyde (MDA) content in leaves and roots of plants exposed to 50 μM and 100 μM Al was significantly higher than in the other groups and the control at 6 to 9 days of treatment. These results suggest that alterations in nucleoli and altered antioxidant enzyme activity and MDA content in V. faba can serve as useful biomarkers for detection of Al toxicity. The mechanisms of Al toxicity and tolerance in V. faba are briefly discussed
EN
The Jurassic-Cretaceous strata of the northern margin of the Dabie Orogen consist of terrestrial sediments comprising thirteen formations, about eight km of thickness at the southern margin of the Hefei-Huangchuan basins, and are divided into four chronosomes. The chronosome I comprises the Lower Jurassic Fanghushan Formation and the lower part of Middle-Upper Jurassic Yuantongshan Formation. The formations represent braided rivers, meandering rivers and marginal to shallow lacustrine environments, and are developed only at the east end of the Hefei Basin. The Chronosome II consists of the Middle-Upper Jurassic Sanjianpu and Zhuji formations, and the upper part of Yuantongshan Formation. The alluvial fans are recognized in the lower parts, and braided rivers in the middle and upper parts of the formations. Transverse water systems crossing the orogen were developed at an early stage, and longitudinal water systems paralleling the orogen in the late stage of sedimentation in the area of study. The East-West stretching Xinyang-Jinzhai-Shucheng Fault (XJSF) borders the sedimentation area in the south. The Chronosome III comprises lower parts of Lower Cretaceous Fenghuangtai, Duanji and Zhougongshan formations, of which the former two consist of alluvial fans and the latter formation of braided rivers and overbank deposits. Coarse-grained siliciclastic deposits prograded into the basins for a few kilometers. The transverse water system developed on the southern margin of the basins, and the longitudinal water system - in the middle of the basins. Chronosome IV comprises the late Early Cretaceous Heishidu and Chenpeng formations. The Xiaotian-Mozitan Fault (XMF) and the Tongbai-Shangcheng Fault (TSF) are placed at the southern boundary of the depositional basin, and the faults are mainly of strike-slip character. Alluvial fans to fan-deltas developed at an early stage, whereas turbidites were deposited in deep lacustrine environments at the late stage of sedimentation. The study area mostly suffered uplift and erosion during the Late Cretaceous; sedimentation was then limited to the western part of the Huangchuan Basin. Longitudinal faults such as XJSF, XMF and TSF paralleling the orogen, and more or less, East-West trending, limited the chronosomes in the south. Transverse faults such as the Tancheng-Lujiang Fault (TLF) and the Shangcheng-Macheng Fault (SMF), more or less North-East trending, controlled lateral facies changes of the chronosomes. Deposition overlapped progressively from east to west at the southern margin of Hefei-Huangchuan basins, which implies that exhumation and unroofing of the Dabie Orogen was diachronous from east toward west. The chronosomes were restricted by TLF and SMF. TLF controlled the development of the Jurassic depocenter, which implies that the fault did not become active until the Early Jurassic.
EN
In nickel sulfide processing, magnesium silicates (serpentines) can easily form slime coatings or hetero-aggregation on pentlandite surfaces, and hence decrease the pentlandite flotation rate and recovery. In this work, magnetic separation of pentlandite from serpentine using selective magnetic coating through adding magnetite fines as magnetic seeds was investigated. Interactions of magnetite-pentlandite and magnetite-serpentine were calculated by the DLVO (Derjaguin-Landau-Verwey-Overbeek) theory. The results show that the interaction of magnetite-pentlandite was obviously stronger than that of magnetite-serpentine with an external weak magnetic field (4776 A/m-1). Therefore, fine magnetite fractions selectively adhered to the pentlandite surfaces and enhanced its magnetism, resulting in being separated from serpentine by magnetic separation, which was further verified by magnetic coating-magnetic separation and SEM observations.
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