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Assessment of biodegradation activity of selected strains of lipolytic bacteria

100%

Latala A. , Wierzba S.

Biotechnologia

2004

nr 3

193-201

The existing experience point out to the fact that the application of microbiological methods to degrade fat contained in organic waste is the most effective method of their biodegradation. The efficacy of this method depends on the rational screening of fat biodegrading microorganisms. The examination assesses the lipolytic activity and the efficacy of waste oil biodegradation by selected lipolytic bacteria. The examination was carried out in laboratory conditions and it assessed such values as lipolytic activity, total bacteria count including lipolytic bacteria and the amount of fatty substances. Both archived strains and strains isolated from organic waste were used in the examination. The highest lipolytic activity was manifested by the bacteria of the Bacillus and Pseudomonas strains, including Bacillus subtilis, Pseudomonas fluorescens and Pseudomonas fragi. For those strains, the fatty substance reduction after 7 days of culturing was between 50 and 60%.

Biological utilisation of fatty waste - initial laboratory examination

80%

Latala A. , Wierzba S. , Latala B.

Biotechnologia

2000

nr 1

124-134

This article presents the process of biodegradation of aliphatic substances in oily bleaching soil. For the experiment, oily bleaching soil from the NZPT (a fat-processing plant) from the town of Brzeg was used. Its characteristic features were a high aliphatic substance content and low (3.8-4.5) pH. By means of experiment three composites of bacteria and yeast were prepared with the use of field and library strains. In a 44-day process, the mixture of field and library strains caused the aliphatic substance content in the oily bleaching soil to drop by 69-71%. The application of bacterial and yeast strains proved efficient for the aliphatic low-pH waste.

Biodegradation of industrial waste with the application of lipolytic, proteolytic and cellulolytic bacteria

70%

Latala A. , Wierzba S. , Farbiszewska T. , Polaczek B. , Boniewska E.

Biotechnologia

2004

nr 3

202-212

The intensive civilization growth has lead to an increase in the amount of industrial waste. One of the ways to reduce the amount of waste is biodegradation. This paper presents the process of industrial waste biodegradation with the use of lipolytic, proteolytic and cellulolytic bacteria. Due to the complex composition of the waste, the degradation of fat, protein and carbohydrates. The biodegradation was carried out with the use of both archived and field bacteria. The aforementioned strains were also used in their genetically modified forms. The examination proved that genetically modified bacteria manifested much better biodegradation capacity than unmodified strains, and that the former reduced 89-94% of fat, 90-95% of protein and 90-96% of carbohydrates after 4 weeks of examination. In the case of unmodified strains, the reduction rates were lower by a few or more percent respectively, regardless of the substrate applied. In all the variations of the examination of reduction of fat, protein and carbohydrates the count of microorganisms was growing. Also, a close dependence between the oxidation and the intensity of the processes examined was noticed.