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EN
Shrimps were exposed to various concentrations of CdCl2 under laboratory conditions for 96 h. Abdominal muscles were isolated from exposed and control animals. The induction of stress proteins (heat shock proteins of the Hsp70 family and metallothionein) was detected following polyacrylamide gel electrophoresis in the presence of SDS (SDS-PAGE) and specific staining (Western blotting method in the case of the Hsp70 or Coomassie blue and silver staining in the case of metallothionein). The short-term cadmium poisoning in the shrimp Crangon crangon resulted in the induction, in a concentration-dependent manner, of metallothionein and a new protein with an approximate molecular weight of 70 kDa in abdominal muscle. This protein was immunologically cross-reactive with the 70 kDa heat shock protein of the mouse.
EN
Previous studies in the Gulf of Gdańsk discussed the responses of selected enzymatic biomarkers to the contaminant gradient in fish and mussels. In the present study, flounder muscle and liver tissues were analyzed for polychlorinated biphenyls (PCB congeners: 28, 52, 101, 118, 138, 153 and 180), organochlorine pesticides (HCHs, HCB and DDTs), and trace metals (Pb, Cd, Zn, Cu, Hg, Cr). An attempt was made to identify the relationship between the measured enzymatic biomarker responses (cholinesterases, malic enzyme, isocitrate dehydrogenase, glutathione S-transferase) and contaminant concentrations in selected flounder tissues. The observed differences in enzymatic biomarker levels suggest that chronic exposure to low-concentration mixtures of contaminants may be occurring in the studied area. However, no conclusive evidence was found of a clear link between the biomarker responses and contaminant concentrations in flounder tissues.
EN
Previous studies in the Gulf of Gdańsk discussed the responses of selected enzymatic biomarkers to the contaminant gradient in fish and mussels. In the present study, flounder muscle and liver tissues were analyzed for polychlorinated biphenyls (PCB congeners: 28, 52, 101, 118, 138, 153 and 180), organochlorine pesticides (HCHs, HCB and DDTs), and trace metals (Pb, Cd, Zn, Cu, Hg, Cr). An attempt was made to identify the relationship between the measured enzymatic biomarker responses (cholinesterases, malic enzyme, isocitrate dehydrogenase, glutathione S-transferase) and contaminant concentrations in selected flounder tissues. The observed differences in enzymatic biomarker levels suggest that chronic exposure to low-concentration mixtures of contaminants may be occurring in the studied area. However, no conclusive evidence was found of a clear link between the biomarker responses and contaminant concentrations in flounder tissues.
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