Wyniki wyszukiwania - Biblioteka Nauki

1

Stanowisko mieczyka dachowkowatego Gladiolus imbricatus L. w Parku Krajobrazowym Chelmy na Pogorzu Kaczawskim

100%

Rombel-Bryzek A.

Chrońmy Przyrodę Ojczystą

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2007

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tom 63

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nr 1

101-105

2

Clinical trials of new therapies for mucopolysaccharidoses

63%

Dewalska A. , Rombel-Bryzek A.

Medical Science Pulse

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2024

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tom 18

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nr 3

3

Wpływ kwasów huminowych na aktywność metaboliczną buraka cukrowego w warunkach suszy

63%

Rombel-Bryzek A. , Pisarek I.

Proceedings of ECOpole

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2017

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tom Vol. 11, No. 1

279--286

PL

Reaktywne formy tlenu (RFT) powstają w komórkach jako produkt uboczny wielu procesów metabolicznych, a ich wytwarzanie nasila się pod wpływem wielu czynników środowiskowych. W pewnych granicach stężeń obecność RFT jest niezbędna do zachowania prawidłowego funkcjonowania komórki, dlatego rośliny wykształciły liczne mechanizmy, których rola polega na utrzymaniu stałego stężenia RFT. Zachwianie równowagi pomiędzy powstawaniem RFT a działaniem ochronnym systemu antyoksydacyjnego prowadzi do stanu zwanego stresem oksydacyjnym. Susza jest głównym czynnikiem ograniczającym wzrost i rozwój roślin. Jednocześnie badania dowiodły, że kwasy huminowe stosowane dolistnie łagodziły negatywne skutki niedoboru wody dzięki specyficznemu aktywowaniu enzymatycznych systemów antyoksydacyjnych. Celem badań była ocena wpływu kwasów huminowych zastosowanych dolistnie na aktywność metaboliczną buraka cukrowego Beta vulgaris L. w warunkach suszy. Badania przeprowadzono w warunkach laboratoryjnych. W materiale roślinnym, który stanowiły rośliny buraka cukrowego, oznaczano aktywność właściwą peroksydazy gwajakolowej (GPOX), stopień peroksydacji lipidów, zawartość chlorofilu a i b. Wyniki ujawniły, że dolistne zastosowanie kwasów huminowych u roślin wzrastających w warunkach suszy nie zwiększa aktywności właściwej GPOX w porównaniu do roślin regularnie podlewanych. Jednocześnie u tych roślin stwierdzono wzrost stopnia peroksydacji lipidów. W badaniach nie stwierdzono wpływu warunków uprawy na zawartość chlorofilu a i b w badanych roślinach.

EN

Reactive oxygen species (ROS) are the byproducts of many metabolic processes and their production is increased under of number of environmental factors. In certain concentration range, the presence of ROS is necessary to maintain proper cell function. Thus, cells have many mechanisms, which role is focused on maintaining a constant concentration of ROS. Imbalance between the formation and action of a protective antioxidant system leads to oxidative stress. Drought is a major limiting factor for plant growth and development. At the same time studies have shown that humic acids foliar application moderated the negative effects of water deficient through activation of the enzymatic antioxidant systems. The aim of the study was to evaluate the effect of humic acid foliar application on the metabolic activity of sugar beet Beta vulgaris L. under drought conditions. Studies were conducted under laboratory conditions. In the plants of sugar beet were determined the specific activity of guaiacol peroxidase (GPOX), lipid peroxidation and chlorophyll a and b content. The results revealed that the humic acid foliar application in plants growing under drought conditions does not increase the specific activity GPOX compared to plants watered regularly. At the same time, found an increase in the degree of lipid peroxidation in these plants. There was no impact of growing conditions on the content of chlorophyll a and b in the studied plants.

4

Wetland restoration enhances the development of protected species (Iris siberica L.) - a case study

63%

Sporek M. , Rombel-Bryzek A.

Polish Journal of Ecology

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2005

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tom Vol. 53, nr 4

591-595

EN

The diminution of biological diversity is widely observed throughout the world in a variety of plant communities. Species protection itself is an insufficient form of preservation of the Siberian Iris population. A serious threat to this species is systematic diminishing of its typical habitat - Molinietum caeruleae plant community, i.e. Molinia meadows on calcareous, peaty or clayey-silt-laden soils. Its maintenance is possible only through conservation and restoration of the plant communities in which it occurs.

5

Wetland restoration enhances the development of protected species [Iris siberica L.] - a case study

63%

Sporek M. , Rombel-Bryzek A.

Polish Journal of Ecology

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2005

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tom 53

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nr 4

EN

The diminution of biological diversity is widely observed throughout the world in a variety of plant communities. Species protection itself is an insufficient form of preservation of the Siberian Iris population. A serious threat to this species is systematic diminishing of its typical habitat – Molinietum caeruleae plant community, i.e. Molinia meadows on calcareous, peaty or clayey-silt-laden soils. Its maintenance is possible only through conservation and restoration of the plant communities in which it occurs.

6

The effect of cadmium on oxidative stress in Beta vulgaris

51%

Rombel-Bryzek A. , Rajfur M. , Żuk O. , Zając P.

Ecological Chemistry and Engineering. S

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2018

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tom Vol. 25, nr 3

457--467

EN

As a heavy metal, cadmium has strongly toxic effects on plants and can induce oxidative stress. It is absorbed by the roots and transported to the stems and leaves. The aim of the study was to evaluate the effect of various concentrations of cadmium on the metabolic activity of Beta vulgaris and assess the dependence of these processes on the content of metal in the plants. To demonstrate the effect of cadmium on metabolism, protein and photosynthetic pigment content, lipid peroxidation, and the activity of enzymes specific for oxidative stress in roots and shoots were measured. Seeds of B. vulgaris were treated with different concentrations of Cd supplied via a CdCl2 solution: 0 (control), 200, 300 and 400 mg/dm3. Results of the present study revealed increased GPOX activity as cadmium concentration rose, while SOD activity was stimulated by a low Cd concentration (200 mg/dm3) and reduced by high levels of Cd. Based on the present findings, it can be concluded that GPOX in B. vulgaris played a more important role in ROS scavenging than SOD did and was able to reduce the level of lipid peroxidation in plants. Cadmium, in the concentration range used, did not show any significant effect on protein or photosynthetic pigment content.

7

Optymalizacja procesu immobilizacji komórek Saccharomyces cerevisiae w żelu alginianowym

51%

Rombel-Bryzek A. , Zhuk O. , Latała A.

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tom Vol. 10, No. 1

289--297

PL

Celem prezentowanych badań była ocena wpływu dwuwartościowych kationów wapnia, magnezu i baru, na zdolność sieciowania żelu alginianowego, stosowanego do unieruchamiania komórek Saccharomyces cerevisiae, oraz oznaczenie aktywności enzymatycznej dehydrogenazy bursztynianowej w immobilizowanych komórkach. Do immobilizacji komórek Saccharomyces cerevisiae wykorzystywano zawiesinę komórek drożdży w alginianie sodu, który następnie żelowano roztworami chlorku wapnia (2, 5, 10 i 30%), chlorku baru (1, 2, 3, 4 i 5%) oraz chlorku magnezu (5%). Aktywność dehydrogenazy bursztynianowej badano spektrofotometrycznie i mierzono za pomocą ilości zredukowanego błękitu metylenowego (sztuczny akceptor elektronów). Uzyskane wyniki ujawniły, że zarówno stężenie, jak i rodzaj stosowanych jonów mają istotny wpływ na stopień usieciowania żelu alginianowego i w konsekwencji na aktywność enzymatyczną SDH w unieruchomionych komórkach. Spośród zastosowanych kationów metali zdolne do sieciowania alginianu były jony wapnia i baru. Ponadto okazało się, że kationy baru charakteryzują się silniejszym powinowactwem do alginianu, dając silniej usieciowany żel alginianowy. Wykazano, że zbyt silne usieciowane alginianu może ograniczać wymianę substancji między żelem alginianowym a roztworem mieszaniny reakcyjnej i, w konsekwencji, zaburzać przebieg reakcji enzymatycznej.

EN

The aim of this study was to evaluate the effect of divalent cations, calcium, magnesium and barium, for the ability to crosslink the alginate gel. The alginate gel was used to immobilize cells of Saccharomyces cerevisiae. The enzymatic activity of succinate dehydrogenase in the immobilized cells was determined. A suspension of yeast cells in sodium alginate, was gelation by solutions of calcium chloride (2, 5, 10 and 30%), barium chloride (1, 2, 3, 4, and 5%) and magnesium chloride (5%). Succinate dehydrogenase activity was tested spectrophotometrically and measured using a reduced amount of methylene blue (an artificial electron acceptor). The results revealed that both the concentration and type of ions employed are important to the degree of cross-linking of the alginate gel and consequently on the enzymatic activity of the SDH immobilized cells. Among the used metal cations capable of crosslinking the alginate are calcium and barium. It was also shown that the barium cations have a stronger affinity for the alginate, to give highly crosslinked alginate gel. It has been shown that too strong cross-linked alginate may limit the exchange of substances between the alginate gel and the solution of the reaction mixture, and consequently interfere with the course of the enzymatic reaction.

8

The impact of copper ions on oxidative stress in garden cress Lepidium sativum

51%

Rombel-Bryzek A. , Rajfur M. , Zhuk O.

Ecological Chemistry and Engineering. S

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2017

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tom Vol. 24, nr 4

627--636

EN

Normal oxygen metabolism is an endogenous source of reactive oxygen species (ROS). The source of ROS are also many environmental factors including heavy metals. In certain concentration range, the presence of ROS is necessary to maintain proper cell function. Thus, cells have many mechanisms, which role is focused on maintaining a constant concentration of ROS. Imbalance between the formation of ROS and action of a protective antioxidant system leads to oxidative stress. This may results with a damage to the structure of proteins, lipids and nucleic acids, which in turn can lead to disturbances in the functioning of the cell and even to the death. The aim of the study was to evaluate the effect of copper ions on the metabolic activity of garden cress Lepidium sativum L. The action of copper ions with different concentrations was treated seeds. After four, six and eight days after planting in the leaves of garden cress were determined the specific activity of guaiacol peroxidase (GPOX), lipid peroxidation and protein content. Additionally intake of copper ions was determined using adsorption spectrometry technique. The results revealed that the applied doses of copper ions affected the activity of guaiacol peroxidase. The highest enzyme activity was found in plant material, which was treated with dose of copper ions 1000 mg/dm3 regardless of day. In the same samples the lowest level of lipid peroxidation was found. The highest concentrations of total proteins was found in samples treated with the highest dose of copper ions. The copper content in the tested plant material is correlated with the applied dose of copper ions. Our results indicate reliable correlations between copper content and values of oxidative stress biomarkers in plant tissues.

9

The influence of methylene blue and malachite green on common carp (Cyprinus carpio) blood parameters

38%

Bojarski B. , Jurecka P. , Szala L. , Kondera E. , Gaj-Chucher C. , Stonawski B. , Rombel-Bryzek A.

Animal Science and Genetics

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2023

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tom 19

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nr 3

10

Does the site of blood collection in fish affect haematological and blood biochemical results?

32%

Bojarski B. , Socha M. , Drag-Kozak E. , Rombel-Bryzek A. , Kapinos S. , Szala L. , Kondera E. , Lugowska K. , Witeska M.

Folia Biologica

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2021

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tom 69

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nr 2

11

The importance of tight junctions in the blood-brain barrier

32%

Kapinos S. , Rombel-Bryzek A. , Chmielewski J. , Adamczyk-Gruszka O. , Grudzien R. , Dziechciaz M. , Strzelecka A. , Nowak-Starz G.

Journal of Elementology

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2021

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tom 26

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nr 4