Flow-through toxicity tests were conducted on common freshwater fishes: rainbow trout (Oncorhynchus mykiss), three-spined stickleback (Gasterosteus aculeatus), roach (Rutilus rutilus), perch (Perca fluviatilis) and dace (Leuciscus leuciscus), to estimate their sensitivity to acute toxicity of nickel. The 96-hour median lethal concentration (96-hour LC50) values obtained from the tests ranged from 19.3 to 61.2 mg Ni/l. According to nickel sensitivity, the species tested may be arranged in the following sensitivity order: rainbow trout > three-spined stickleback > perch = roach > dace. Obtained data could be successfully applied in solving theoretical and practical goals of aquatic toxicology.
Our study investigated the behavioural responses of medicinal leech and rainbow trout at different ontogenetic levels under the effect of crude oil (CO) and heavy fuel oil (HFO), performed comparative analysis of the sensitivity of animal responses, evaluated the specificity of these responses, and determined “safe“ toxicant concentrations. Comparison of sensitivity of behavioural responses of leech and fish revealed that the most sensitive response to long-term exposure to CO was leech locomotor activity, while the most sensitive parameter to HFO was the coughing rate in juvenile fish. Our study showed that the sensitivity and specificity of behavioural responses of aquatic animals at different phylogenetic and ontogenetic levels can be successfully used to evaluate the toxicity of ambient water polluted with oil hydrocarbons.
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