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Frequent failures in the treatment of human and animal infections with antibiotics give little reason for optimism as to the perspectives of this kind of therapy. It is estimated that biofilms, which are the most common factor responsible for such diseases, demonstrate up to 1000-fold higher drug resistance than dispersed populations of the same bacteria species. A relatively slow development of new antibiotics suggests the need for a new approach in anti-infection therapy, alternative to the therapy with “classical” antibiotics. There is a growing interest in applying natural components of plant and animal immune systems. Nowadays, a challenge for medicine and microbiology is to discover new agents that would increase the effectiveness of anti-film therapy. These studies could verify current views on biofilms and their role in some infectious diseases of humans and animals.
EN
Pseudomonas spp., are Gram-negative rods belonging to the Pseudomonadaceae family. These bacteria often change their phenotypic characteristics, are very adaptable and also resistant to most of the utilized antibiotics and disinfectants. The major representative of these bacteria is Pseudomonas aeruginosa (blue pus rod), which is primarily considered as a dangerous pathogen not only to humans but also for animals. P. aeruginosa is isolated from the gastrointestinal tract but these bacteria also constitute physiological microflora of the semen. Strains with increased virulence present in semen may involve the reproductive processes of the utilized breeds. These microorganisms are also used in ecology for the decomposition of oil-derivative components.
EN
The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.
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