Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Secretion of stress-related proteins by suspension-cultured Lupinus albus cells

100%

Wojtaszek P. , Pislewska M. , Bolwell G. P. , Stobiecki M.

nr 1

A suspension culture of white lupin cells has been established, and proteins of the exocellular matrix analysed. Based on homologies of N-terminal amino-acid sequences, three stress- or defence-related proteins: acidic class III chitinase, polygalacturonase-inhibiting protein, and germinóxalate oxidase, secreted by lupin cell culture, were identified.

Exocellular [glyco]proteins of Lupinus albus plants and suspension-cultured cells

100%

Wojtaszek P. , Pislewska M. , Bolwell G. P.

tom 20

nr 3

Plant species from genus Lupinus are among the oldest known legumes, and various aspects of their biology are considerably different from those commonly observed within Leguminosae. To study this issue in more detail, a suspension culture of Lupinus albus cells was developed, and the glycosylation patterns of exocellular proteins analysed. N-linked oligosaccharide side-chains were detected with two lectins: concanavalin A (ConA) and wheat germ agglutinin (WGA) used with respective anti-lectin antibodies, while O-linked arabinosylated side-chains of (hydroxy)proline-rich glycoproteins were identified with anti-(42 kDa French bean chitin-binding protein) antibodies. The obtained data were compared with analogous ones for exocellular (glyco)proteins from suspension-cultured Phaseolus vulgaris cells and from various tissues of L. albus plants. Major species-specific differences between exocellular (glyco)proteins from lupin and bean cells were identified. Similarly, developmentally regulated glycosylation changes following transition from organised plant tissue to dedifferentiated suspension-cultured lupin cells were detected and analysed.

Exocellular pathogenesis defence-related [glyco]proteins from suspension-cultured Lupinus albus cells

100%

Wojtaszek P. , Pislewska M. , Bolwell G. P. , Stobiecki M.

Biological Bulletin of Poznań. Supplement

1997

tom 34