Wyniki wyszukiwania - Biblioteka Nauki

1

Redescription of a poorly-known marine cyrtophorid ciliate, Dysteria pusilla [Claparede et Lachmann, 1859] [Protozoa: Ciliophora: Cyrtophorida] from Qingdao, China

100%

Gong J. , Lin X. , Song W.

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nr 3

2

Two new marine pleurostomatid ciliates from China, Loxophyllum jini and L. qiuianum [Ciliophora: Pleurostomatida]

100%

Lin X. , Song W. , Wilbert N. , Warren A.

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nr 2

3

On two marine oligotrich ciliates, Spirostrombidium agathae n. sp. and S. schizostomum [Kahl, 1932] n. comb. from China, with a key to the identification of seven well-characterized Spirostrombidium spp. [Ciliophora: Oligotrichida]

100%

Xu D. , Song W. , Lin X. , Warren A.

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tom 45

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nr 4

4

Identification of genes associated with fruit ripening in Ziziphus jujuba using suppression subtractive hybridization approach

88%

Lin X. , Yao L. , Li Y. , Wu R. , Pang X.

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nr 06

EN

Owing to the high nutritional value and extensive medicinal use of its products, Chinese jujube (Ziziphus jujuba Mill) is one of the most important fruit crops in China. However, jujube fruits are highly perishable and thus have a short shelf life, which is a serious hindrance to the industry. Better understanding of the molecular mechanisms underlying jujube fruit softening is fundamental to overcome the problem. Thus, both forward and reverse suppression subtractive hybridization (SSH) cDNA libraries were constructed to identify differentially expressed genes for fruit at half-red ripening stage and complete red stage. As a result of dot blot confirmation, a total of 154 differentially expressed genes were identified. After removed low-quality regions and screened for vector contamination, blasted with the non-redundant NCBI databases, 78.6 % of sequences exhibited high homology to previously identified or putative proteins. All the ESTs were annotated and classified according to the terms of the three main Gene Ontology vocabularies using the Blast2GO software. Furthermore, the quantitative real-time PCR was carried out for 17 genes to validate the genes differentially expressed from the SSH libraries. And the full-length sequences of galactose oxidase and aldehyde dehydrogenase genes were obtained. It is the first step to explore the functional genomics and regulatory networks during the storage period of jujube fruit. The identification of the genes differentially expressed is helpful to understand the ripening and softening of the jujube fruit at the molecular level.

5

DNA methylation polymorphism in a set of Elite rice cultivars and its possible contribution to inter-cultivar differential gene expression

88%

Wang Y. , Lin X. , Dong B. , Wang Y. , Liu B.

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nr 3

EN

RAPD (randomly amplified polymorphic DNA) and ISSR (intersimple sequence repeat) fingerprinting on HpaII/MspI-digested genomic DNA of nine elite japonica rice cultivars implies inter-cultivar DNA methylation polymorphism. Using both DNA fragments isolated from RAPD or ISSR gels and selected low-copy sequences as probes, methylation-sensitive Southern blot analysis confirms the existence of extensive DNA methylation polymorphism in both genes and DNA repeats among the rice cultivars. The cultivar-specific methylation patterns are stably maintained, and can be used as reliable molecular markers. Transcriptional analysis of four selected sequences (RdRP, AC9, HSP90 and MMR) on leaves and roots from normal and 5-azacytidine-treated seedlings of three representative cultivars shows an association between the transcriptional activity of one of the genes, the mismatch repair (MMR) gene, and its CG methylation patterns.

6

Nutritional components and antioxidant activities of Firmiana platanifolia seeds in response to different germination times

76%

Zhi X. M. , Qian W. Z. , Song Y. , Mu C. J. , Yu D. S. , Lin X. , Gao S.

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tom 29

|

nr 1

7

Prostate cancer stem cells: viewing signaling cascades at a finer resolution

76%

Lin X. , Farooqi A. A. , Qureshi M. Z. , Romero M. A. , Tabassum S. , Ismail M.

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2016

|

tom 64

|

nr 3

8

Mitochondrial uncoupling protein silence is compromised in photosynthesis and redox poise

76%

Chen S. , Jin W. , Liu A.-r. , Liu Z. , Liu D. , Xia X. , Lin X.

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nr 08

EN

Mitochondrial uncoupling proteins play important roles in generation of metabolic thermogenesis, response to stress situations, and regulation of energy metabolism. We demonstrated here that the absence of LeUCP in tomato inhibited photosynthesis using virusinduced gene-silencing approach. A significant decrease in the rate of CO2 assimilation in LeUCP-silencing plants was observed over a range of different light intensities. Absence of LeUCP resulted in lower net photosynthetic rate, lightsaturated rate of the CO2 assimilation (Asat), maximum carboxylation rates (Vcmax) and maximum RuBP regeneration rate (Jmax). Activities of ribulose-1,5-bisphosphate carboxylase/oxygenase Rubisco and stromal fructose-1,6- bisphosphatase and genes expression levels encoded Calvin cycle enzymes of LeUCP gene-silencing plants were inhibited. However, silencing of LeUCP gene had no effect on Fv/Fm, but decreased photochemical quenching and electron transport rate. Meanwhile, non-photochemical quenching and Je (PSII), the distribution of Je (PCR) and Je (PCO), the content of AsA, NAD, and the ratios of NAD?/ NADH, AsA/DHA were significantly reduced with increased reactive oxygen species while GSH and GSSG were unaltered. Taken together, these results strongly suggest that LeUCP gene in tomato leaves is crucial in maintaining the redox poise of the mitochondrial electron transport chain to facilitate photosynthetic metabolism.