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1
Biodegradowalne poliestry hydroksykwasow alkanowych [PHA] syntetyzowane przez drobnoustroje
100%
Szewczyk E. M.
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nr 3
97-115
2
Perspektywy syntezy bakteryjnych biodegradowalnych poliestrow przez rosliny
100%
Szewczyk E. M.
Kosmos
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1995
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tom 44
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nr 3-4
709-718
3
Wytwarzanie substancji przeciwbakteryjnych przez maczugowce rezydujace na skorze czlowieka
63%
Kwaszewska A. , Szewczyk E. M.
Medycyna Doświadczalna i Mikrobiologia
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2007
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tom 59
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nr 3
251-257
4
Zdolnosc adhezji do komorek nablonkowych i powierzchni tworzyw szczepow Staphylococcus cohnii zasiedlajacych srodowisko szpitalne
63%
Waldon E. , Szewczyk E. M.
Medycyna Doświadczalna i Mikrobiologia
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2002
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tom 54
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nr 2
109-118
PL
Badano zdolność adhezji szczepów Staphylococcus cohnii do komórek nabłonkowych człowieka i do powierzchni tworzyw. Stwierdzono zdolność adhezji tych bakterii do komórek nabłonka, szczególnie wśród szczepów podgatunku S. cohnii ssp. cohnii oraz silnie wyrażoną właściwość większości badanych szczepów do wiązania się z powierzchnią tworzyw sztucznych i szkła. Oceniano związek tych cech z hydrofobowością i wytwarzaniem wewnątrzkomórkowego śluzu.
EN
Presented study describes abilities of staphylococci to adhere to exfoliated cheek and uroepithelial epithelium cells and to various surfaces such as plastics, glass and steel. The subject of the study were strains of Staphylococcus cohnii ssp. cohnii and Staphylococcus cohnii ssp. urealyticus isolated from Intensive Care Unit of Pediatric Hospital. Staphylococcus cohnii ssp.cohnii adhered in great number to epithelial cells. However, the adhesion differed by individual strains. We did not find relationship between slime production and adherence to epithelial cell. Most of investigated strains adhered closely to surfaces - especially of plastics and glass. This phenomenon was stronger in the presence of culture medium and phosphate buffer.
5
Wplyw warunkow badania na ocene wrazliwosci Staphylococcus cohnii na antybiotyki beta-laktamowe
63%
Nowak T. , Szewczyk E. M.
Medycyna Doświadczalna i Mikrobiologia
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2006
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tom 58
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nr 2
95-102
PL
Przy oznaczaniu wrażliwości Staphylococcus cohnii na antybiotyki ß-lakta- mowe zastosowanie warunków hodowli stymulujących mechanizmy oporności sprzyja eliminacji błędnych wyników, niezgodnych z dobrze sprawdzającymi się wynikami oznaczania meticylinooporności w krążkowym teście z cefoksytyną (FOX30).
EN
The high occurence of coagulase-negative staphylococci among bacteria responsible for hospital infections is unquestioned. Studies on the poorly-known novobiocin-resistant, coagulase-negative Staphylococcus cohnii were undertaken. The possibilities of optimizing conditions for determination of susceptibility to ß-lactam antibiotics of this species were researched. In the case of S. cohnii the new cefoxitin test for detection of methicillin resistant strains, introduced by the National Reference Centre for Antibiotics in Poland was found as a good and of credible quality. It was also shown, that application in in vitro examination conditions stimulating the mechanisms of resistance to ß-lactam antibiotics, supplies credible results relating to their true susceptibility. The necessity of establishing individual conditions for susceptibility determination in different species of coagulase-negative staphylococci was suggested.
6
Gronkowce koagulazoujemne w odpadach komunalnych
63%
Sobis-Glinkowska M. , Szewczyk E. M.
Medycyna Doświadczalna i Mikrobiologia
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2004
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tom 56
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nr 1
19-27
PL
Aerozole powstające podczas utylizacji odpadów komunalnych zawierają między innymi koagulazoujemne gronkowce, spośród których wiele wykazuje oporność na antybiotyki. Przy obróbce odpadów następuje selekcja szczepów opornych na tetracyklinę i chloramfenikol.
EN
The total of 259 coagulase-negative staphylococci isolates gained from aerosols created during dumping and utilisation of municipal waste were investigated. Species and subspecies identification allowed to determine predominance of novobiocin-resistant species which primarily colonise rodents or other animals. However, about 30% of isolates were species originating from humans. Metabolic properties of the studied group of isolates were examined, as well as their sensitivity to a set of 8 commonly used antibiotics: amoxicillin, erythromycin, tetracycline, clindamicin, chloramphenicol, gentamicin, trimethoprim/sulfamethoxazole and ciprofloxacin. Many isolates resistant to erythromycin (19%), tetracycline (13%) and a considerably smaller group resistant to clindamycine (6.2%), chloramphenicol (2%) and trimethoprim/sulfamethoxazole (1%) were found. It appeared that processes of occurring at the time of dumping and utilisation of waste affect the prevalence of only some features. The examined metabolic features were found to be relatively stable and no changes were observed, which would indicate the tendency of adaptation to existence in the inanimate matter environment. Only isolates with active nitrate reductase were more frequently detected. The increase in frequency of occurrence of isolates resistant to tetracycline and chloramphenicol and simultaneous elimination of isolates resistant to other antibiotics were observed.
7
Cytolizyny – czynniki zjadliwości Staphylococcus intermedius i Staphylococcus pseudintermedius
63%
Kmieciak W. , Szewczyk E. M.
|
|
nr 4
8
Bacteria forming a resident flora of the skin as a potential source of opportunistic infections
63%
Kazmierczak A. K. , Szewczyk E. M.
Polish Journal of Microbiology
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2004
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tom 53
|
nr 4
EN
Along with progress of medicine, contribution that opportunistic bacteria make in nosocomial infections increases. Coagulase-negative staphylococci are these multiresistant strains which often cause this kind of infections. But more and more frequently other genera of bacteria are isolated. The main source of them is first and foremost the hospitalized patient's endogenous flora e.g. from their skin, because transmission of bacteria from this source is very effective. Analysis was concerned with bacteria that were recovered repeatedly from the skin of young, healthy men during period of five months. Composition of resident bacteria, after removing transients was evaluated. The number of microorganisms per 1 cm² patients' skin was a constant value but different for each patient. Newly composed media enabled exact isolation and qualitative analysis of all groups of expected bacteria. Isolated microorganisms represented three main groups: sensitive to novobiocin staphylococci, microaerophilic rods from Propionibacterium genus and coryneform bacteria. Aside from quantitative differences in total bacteria number, significant differences in contribution of aerobic and anaerobic flora living on patient skin were observed. A persistent although not predominant population occurring on the skin of all patients in similar number (average 2%), were coryneform bacteria. They mainly belonged to the Corynebacterium genus, and 84.7% of them were the lipophilic species. These bacteria deserve special attention because among such species isolated from nosocomial infections, multiple antibiotic resistance of unknown origin was described.
9
Staphylococcus cohnii - resident of hospital environment: cell-surface features and resistance to antibiotics
63%
Szewczyk E. M. , Rozalska M.
|
|
tom 49
|
nr 2
10
Outpatient antibiotic consumption fluctuations in a view of unreasonable antibacterial therapy
51%
Ciszewski M. , Czekaj T. , Szewczyk E. M.
Polish Journal of Microbiology
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2017
|
tom 66
|
nr 1
EN
Unreasonable antibacterial therapy is suspected to be the main reason of emergence of multi-resistant bacteria. The connection between seasonal variability of antibiotic use and reasonable antibacterial therapy has been described. We examined the issue basing on the data obtained from the primary care system in Szczecin (Poland) in order to verify the situation in this region of Central Europe. Increase in antibiotic consumption in a viral infection season was proved to be statistically significant. Statistically significant differences in various drug forms dispensation were also observed. Increased consumption of antibiotics in seasons of influenza-like illnesses might be connected with a lack of proper diagnostics or numerous cases of bacterial co-infections.
11
Species-specific sensitivity of coagulase-negative staphylococci to single antibiotics and their combinations
51%
Szymanska G. , Szemraj M. , Szewczyk E. M.
|
|
tom 60
|
nr 2
EN
The activity of β-lactam antibiotics (oxacillin, cloxacillin, cephalotin), vancomycin, gentamicin and rifampicin applied in vitro individually and in combination against 37 nosocomial methicillin-resistant strains of coagulase-negative staphylococci (CNS) was assessed to demonstrate the heterogeneity of this group of bacteria and estimate the chance of the efficacy of such therapy. The strains belonged to four species: Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus cohnii, Staphylococcus hominis. They originated from a hospital environment and from the skin of medical staff of the intensive care unit of a paediatric ward at a university hospital. All strains were methicillin-resistant, according to CLSI standards, but individual strains differed in MICOX values. Susceptibility to other tested antibiotics was also characteristic for the species. The increased susceptibility to antibiotics in combinations, tested by calculating the fractional inhibitory concentration (FIC) index, concerned 26 out of 37 investigated strains and it was a feature of a particular species. Combinations of vancomycin and cephalotin against S. epidermidis and oxacillin with vancomycin were significant, as well as cephalotin and rifampicin in growth inhibition of multiresistant S. haemolyticus strains.
12
Oddziaływanie substancji wydzielanych przez lipofilny szczep Corynebacterium CDC G1 ZMF 3P13 na inne drobnoustroje bytujące na skórze człowieka
51%
Wysocki P. , Kwaszewska A. K. , Szewczyk E. M.
Medycyna Doświadczalna i Mikrobiologia
|
2011
|
tom 63
|
nr 1
13
New gene responsible for resistance of clinical corynebacteria to macrolide, lincosamide and streptogramin B
51%
Szemraj M. , Kwaszewska A. , Szewczyk E. M.
Polish Journal of Microbiology
|
2018
|
tom 67
|
nr 2
EN
The subject of the study was phenotypic marking of the antibiotic susceptibility and MLSB resistance mechanism in Corynebacterium spp. isolated from human skin (18 isolates) and from clinical materials (19 isolates). The strains were tested for the presence of the erm(A), erm(B), erm(C), erm(X), lnu(A), msr(A), msr(B) and mph(C) genes. Clinical isolates showed wide resistance to antibiotics. In 89% clinical isolates and 72% skin microbiota a constitutive type of MLSB resistance was found. In 12 clinical isolates the erm(C) gene was detected-eight of which had erm(X) as well as erm(C), two harboured erm(X), erm(C) and erm(A) and two demonstrated only erm(C).
14
Wplyw kontaktu ze srodowiskiem szpitala na wrazliwosc na antybiotyki i chemioterapeutyki lipofilnych szczepow Corynebacterium rezydujacych na skorze czlowieka
51%
Kwaszewska A. K. , Sobis-Glinkowska M. , Szewczyk E. M.
Medycyna Doświadczalna i Mikrobiologia
|
2009
|
tom 61
|
nr 4
359-366
15
Opportunistic coryneform organisms - resident of human skin
51%
Kazmierczak A. K. , Szarapinska-Kwaszewska J. K. , Szewczyk E. M.
|
|
nr 1
EN
Opportunistic infections are usually caused by endogenic flora originated from physiological flora. In this context we studied coryneform bacteria recovered from deeper layers of epidermis of the forehead (278 isolates) and the back (196 isolates) of healthy men. It was observed that coryneform bacteria are in dynamic equilibrium with coagu-lase-negative staphylococci and they amount 4,7% of resident aerobic flora. On the base of biological and metabolic features 49 different biotypes were indicated. Biotypes of lipophilic rods were the basic part of coryneform flora existing for long period. The most frequently isolated taxa were C. jeikeium (31%), CDC group G2 (23,4%), next in order CDC group Gl (11%) and C. afermentans ssp. lipophilum (7%). These isolates were phenotypically differentiated. Nonlipophilic species did not play significant role in creating resident flora of the skin. The significance of coryneform bacteria in opportunistic infections is growing, especially in immunocompromissed patients. Isolated lipophilic taxa belong to these taxa coryneforms which are described in literature as the main etiologic factors of opportunistic infections.
16
Content available The effect of fragmentation and packaging of dried parsley leaves on selected chemical and microbiological parameters
38%
Sikorska-Zimny K. , Wojcicka K. , Rochminska A. , Rutkowski K. P. , Szewczyk E. M. , Lisiecki P.
|
|
nr 2
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