Wyniki wyszukiwania - Biblioteka Nauki

1

Wystepowanie genow opornosci na metycyline w szczepach Staphylococcus aureus

100%

Bystron J. , Lis E. , Bania J. , Molenda J.

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tom 07

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nr 2

3-8

2

Wytwarzanie enterotoksyn gronkowcowych w żywności

100%

Schubert J. , Krakowiak S. , Bania J.

Medycyna Weterynaryjna

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2018

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tom 74

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nr 01

PL

Staphylococcal food poisoning results from ingestion of food contaminated with toxins produced by enterotoxigenic Staphylococcus aureus strains. Common symptoms of this intoxication include vomiting, diarrhea, and abdominal cramps. Staphylococcal enterotoxins are resistant to heat and a number of environmental factors. Certain cheeses, milk powder, and whey powder are the only foodstuffs that are being routinely examined for the presence of staphylococcal enterotoxins SEA-SEE. The newly identified enterotoxins are not included in the current examination scheme. Enterotoxin-producing staphylococci were already isolated from meat, meat products, milk, dairy products, fermented food products, vegetables, pastries and fish products. It has been demonstrated that many environmental factors associated with food processing and storage can significantly influence the level of secreted enterotoxins by S. aureus strains. Nevertheless, only a few studies on the production of staphylococcal enterotoxins were conducted in foodstuffs. Most data on their expression is based on experiments performed with a low number of S. aureus strains, and usually only SEA-SEE enterotoxins are investigated. These results inclined many authors to the conclusion that milk and dairy products are unfavorable environments for expression of staphylococcal enterotoxins. However, recent research has indicated a significant heterogeneity in the ability of enterotoxin production in milk among S. aureus strains derived from diverse sources. S. aureus strains able to secrete high levels of enterotoxins in milk and meat juice were described. This research indicates that a high number of S. aureus strains should be used for studying staphylococcal enterotoxins expression in food. It seems to be the appropriate way to assess the risk of staphylococcal food poisoning.

3

Prevalence of antibiotic resistance genes in staphylococci isolated from ready-to-eat meat producs

100%

Podkowik M. , Bystron J. , Bania J.

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nr 2

EN

Prevalence of mecA, blaZ, tetO/K/M, ermA/B/C, aph, and vanA/B/C/D genes conferring resistance to oxacillin, penicillin, tetracycline, erythromycin, gentamicin, and vancomycin was investigated in 65 staphylococcal isolates belonging to twelve species obtained from ready-to-eat porcine, bovine, and chicken products. All coagulase negative staphylococci (CNS) and S. aureus isolates harbored at least one antibiotic resistance gene. None of the S. aureus possessed more than three genes, while 25% of the CNS isolates harbored at least four genes encoding resistance to clinically used antibiotics. In 15 CNS isolates the mecA gene was detected, while all S. aureus isolates were mecA-negative. We demonstrate that in ready-to-eat food the frequency of CNS harboring multiple antibiotic resistance genes is higher than that of multiple resistant S. aureus, meaning that food can be considered a reservoir of bacteria containing genes potentially contributing to the evolution of antibiotic resistance in staphylococci.

4

Bakterie z rodzaju Arcobacter - potencjalnym zagrożeniem bezpieczeństwa żywnosci

100%

Bystron J. , Krupa P. , Podkowik M. , Bania J.

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tom 70

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nr 01

EN

The genus Arcobacter was included in the family Campylobacteraceae in 1991, and currently consists of fourteen species, of which A. butzlerii, A. cryaerophilus, and A. skirrowii have been associated with human and animal diseases. Arcobacter spp. have been isolated from food, mainly from products of animal origin, with the highest prevalence in chickens, followed by pork and beef. The bacteria have been commonly detected in chicken carcasses and in the intestinal content of birds. The faecal contamination is regarded as the main route of transmission of Arcobacter spp. into poultry carcasses. Additionally, these bacteria can attach to water pipes and are able to survive in the slaughterhouse environment under chilled conditions. Apart from poultry and other meat, Arcobacter spp. have been isolated from drinking water reservoirs, sewage, faeces of healthy animals and from animals affected by various diseases, including abortion, mastitis, septicaemia, and enteritis. Recent evidence suggests that Arcobacter spp., especially A. butzleri, may be involved in human enteric diseases. Occasionally, these bacteria have also been found in cases of human extraintestinal diseases. Until present, little is known about the infection dose, mechanisms of pathogenicity, and virulence factors of Arcobacter spp. Consumption of raw or poorly cooked contaminated food of animal origin, mainly poultry, is the most likely source of human poisoning. The actual role of Arcobacter spp. in human diseases is probably underestimated because of the lack of standardized identification methods and routine detection procedures.

5

Enzymy hydrolityczne entomopatogennych grzybow i ich rola w patogenezie

100%

Kolaczkowska M. , Chrzanowska J. , Bania J.

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tom 44

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nr 6

31-44

EN

Entomopathogenic fungi attack the host insect and therefore may be potentially used for insect control. Penetration of cuticle is achieved by a combination of mechanical pressure and enzymatic degradation. The entomopathogenic fungi produce a variety of exocellular hydrolytic enzymes: proteases, chitinases and lipases, which are capable to digest the major components of insect cuticle. This review describes the characteristics of these enzymes and their role in pathogenesis.

6

Genotypes of Giardia duodenalis in household dogs and cats from Poland

100%

Piekarska-Stepinska A. , Piekarska J. , Goryczkowski M. , Bania J.

Acta Parasitologica

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2021

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tom 66

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nr 2

7

Enterotoksyny gronkowcowe w żywności - nowe zagrożenia

100%

Podkowik M. , Schubert J. , Bania J. , Bystron J.

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nr 05

8

Application of a duplex-PCR for detection of cows' milk in goats' milk

100%

Kotowicz M. , Adamczyk E. , Bania J.

Annals of Agricultural and Environmental Medicine

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2007

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tom 14

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nr 2

EN

A duplex-PCR method, with 2 pairs of primers recognizing sequences of mitochondrial D-loop region, was developed to identify cows’ milk in the milk of goats. The PCR was shown to be specifi c and sensitive, enabling the detection of less than 1% of cows’ milk added to the milk of goats. Simultaneous use of a primer pair for goats’ and cows’ mitochondrial DNA fragment prevented false negative results. The method was applied to track the presence of cow DNA in goat milk available on the Polish market. A total of 54 milk samples from 3 Polish (34) and one foreign producer (20) were examined. In 33 samples, cow DNA was detected, while 21 samples, including all of the 20 samples from foreign producers, produced the goat-specific product only.

9

Distribution of enterotoxin genes in Staphylococcus aureus isolates from pork and pigs

88%

Bystron J. , Podkowik M. , Bania J. , Krupa P. , Schubert J.

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nr 06

EN

Aim of the study: The study was conducted to determine the incidence of genes encoding emetic staphylococcal enterotoxins (SEs) in S. aureus isolates from pork and pigs, and to demonstrate the connection between the enterotoxigenic potential of S. aureus and its genetic background. Materials and methods: S. aureus isolates from pork (45 isolates) and pigs (45 isolates), representing various clonal complexes, were tested for the presence of emetic SEs genes. Results and discussion: Thirty-four of the 45 S. aureus isolates (75%) derived from pork were shown to harbor genes encoding emetic SEs. Among 45 pig-derived S. aureus isolates, SE genes were detected in 28 isolates (62%). Fifty-five percent of potentially enterotoxigenic staphylococci carried genes encoding classical toxins (SEA-SEE), whereas 28 isolates (45%) harbored exclusively genes encoding new emetic SEs. The most prevalent (82%) classical enterotoxin gene was seb, whereas seg and sei genes dominated (82%) among isolates harboring genes encoding other emetic toxins. Seventeen of 23 S. aureus isolates assigned to the CC15 clonal complex were found to harbor the seb gene. Ten of 15 CC7 isolates contained the selp gene. Isolates harboring seg and sei genes dominated in CC30 (81%) and CC9 clones (76%). Four isolates assigned to CC398 were shown to harbor enterotoxin genes, such as seb, sed, seg, sei, and ser. Our results indicate a high incidence of enterotoxigenic S. aureus isolates harboring genes encoding other emetic SEs in pork and pigs. In most of the pig- and pork-derived isolates studied here, genotype-enterotoxin association was similar to that known from human S. aureus isolates. This is the first report on SE genes in S. aureus CC398 genetic background in Poland, and probably also in Europe.

10

Morphology and molecular study of Fascioloides magna – a growing threat to cervids (Cervidae) in Poland

88%

Houszka M. , Piekarska J. , Podkowik M. , Gorczykowski M. , Bania J.

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nr 4

11

Prevalence and antimicrobial resistance of Arcobacter butzleri and Arcobacter cryaerophilus isolates from retail meat in Lower Silesia region, Poland

88%

Zacharow I. , Bystroń J. , Wałecka-Zacharska E. , Podkowik M. , Bania J.

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nr 1

12

Dystrybucja genow enterotoksyn gronkowcowych wsrod szczepow Staphylococcus aureus wyizolowanych z zywnosci i od ludzi

88%

Bystron J. , Bania J. , Molenda J. , Kosek-Paszkowska K. , Korzekwa K.

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tom 61

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nr 11

1270-1273

13

Invasiveness of Listeria monocytogenes strains isolated from animals in Poland

75%

Walecka-Zacharska E. , Kosek-Paszkowska K. , Bania J. , Staroniewicz Z. , Bednarski M. , Wieliczko A.

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nr 4

EN

Animals are important reservoir of Listeria monocytogenes, a pathogen causing serious infections in both humans and livestock. However, data on invasiveness of L. monocytogenes strains of animal origin is very scarce. Ability of 18 L. monocytogenes strains of animal origin to invade HT- 9 cells was investigated. Plaque forming assay was used to assess invasiveness and ability of the pathogen to spread in the cell line. Almost 40% of L. monocytogenes strains were weakly invasive. It was shown that strains from serogroup 4b exhibited the highest invasiveness, whereas serogroup 1/2b consisted of strains of invasiveness below 0.0001%. Analysis of translated inlA and inlB gene sequences revealed no premature stop codons. Lineage-specific mutations in low invasive strains were identified within inlA and inlB sequences. Our results demonstrate high incidence of low invasive animal L. Monocytogenes strains, which may be at least partly explained by unique point mutations in the InlA and InlB.

14

Reduction of prevalence of persistent BVDV infection in cattle herds by long-term vaccination program (preliminary clinical study)

75%

Rypula K. , Ploneczka-Janeczko K. , Bania J. , Walecka E. , Bierowiec K. , Rozpedek W.

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nr 2

EN

Effectiveness of long-term anti-BVDV vaccination program in reducing prevalence of persistent BVDV infection in cattle herds was evaluated in seven years observational study (2005-2011). Among three seropositive dairy cattle herds (within herd seroprevalence 100%, confirmed by ELISA Herd Check BVDV Ab, IDEXX, Sweden) vaccination program based on inactivated vaccine (cytopathic strain 5960) was commenced in 2007 in two herds and continued till 2010. In the years 2007-2011 all calves aged 2-12 weeks in all three herds were tested yearly with RT-PCR in order to detect persistently infected individuals. For the entire study period true prevalence of BVDV persistent infection was significantly lower in vaccinated than in non-vaccinated herd. This may imply the role of long-term vaccination program in reducing prevalence of persistent BVDV infection in cattle herds.

15

Invasiveness of Listeria monocytogenes strains isolated from animals in Poland

75%

Wałecka-Zacharska E. , Kosek-Paszkowska K. , Bania J. , Staroniewicz Z. , Bednarski M. , Wieliczko A.

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nr 4

16

Detection of enterotoxigenic Staphylococcus aureus strains using a commercial ELISA and multiplex-PCR

75%

Bystron J. , Bania J. , Zarczynska A. , Korzekwa K. , Molenda J. , Kosek-Paszkowska K.

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nr 3

17

Prevalence of antibiotic resistance genes in staphylococci isolated from ready-to-eat meat products

63%

Podkowik M. , Bystroń J. , Bania J.

|

18

Effect of short-term rapid ventricular pacing followed by pacing interruption on arterial blood pressure in healthy pigs and pigs with tachycardiomyopathy

51%

Skrzypczak P. , Noszczyk-Nowak A. , Zyśko D. , Pasławska U. , Janiszewski A. , Gajek J. , Nicpoń J. , Kiczak L. , Bania J. , Zacharski M. , Tomaszek A. , Jankowska E. A. , Ponikowski P. , Witkiewicz W.

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nr 1

19

Matrix metalloproteinase 9/neutrophil gelatinase associated lipocalin/tissue inhibitor of metalloproteinases 1 complexes are localized within cardiomyocytes and serve as a reservoir of active metalloproteinases in porcine female myocardium

51%

Kiczak L. , Tomaszek A. , Bania J. , Paslawska U. , Zacharski M. , Janiszewski A. , Rybinska I. , Dziegiel P. , von Haehling S. , Ardehali H. , Jankowska E. A. , Ponikowski P.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 3

20

P-R interval in porcine model of chronic tachycardia-induced cardiomyopathy

51%

Skrzypczak P. , Zysko D. , Paslawska U. , Noszczyk-Nowak A. , Janiszewski A. , Kiczak L. , Bania J. , Zacharski M. , Tomaszek A. , Rybinska I. , Nicpon J. , Paslawski R. , Gajek J. , Jankowska E. A. , Ponikowski P.

Bulletin of the Veterinary Institute in Puławy

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2013

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tom 57

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nr 3

EN

The aim of the study was to assess the atrioventricular conduction in the model of porcine pacing induced tachycardiomyopathy. Fifty-one swine were examined: 27 were paced and 24 served as a control group. Every 4 weeks, the animals were anaesthetised for 1 h and an ECG Holter was performed. Thirty minutes after the onset of anaesthesia, P-R and R-R intervals were measured. Each result was assigned to the subgroup according to the animal's weight and the presence or absence of previous pacing. P-R interval was longer in animals after at least 4 weeks of rapid ventricular stimulation than in adjusted group of the animals according to the body mass. Multivariate analysis has showed that longer P-R interval was related to male gender, higher body mass, slower heart rate, and history of previous pacing. Chronic ventricular pacing led to the slowing of atrioventricular conduction. The presence of differences in the duration of R-R intervals between groups was only found in swine weighing 120-139 kg. The R-R interval was shorter in paced animals, whereas PR interval was longer in that group, indicating that PR prolongation is related to electrical or structural remodelling of the cardiac conductive tissue but not increased sympathetic nervous system activity, which is expected to produce corresponding changes in PR and R-R intervals.