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tom 44
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nr 06
RU
Определили сосуществование 381 результата проб молока, исследуемого методом Уайтсайда, содержащего консервирующее средство в виде глицерина и борной кислоты. Для сравнительных целей исследовали те же самые пробы без консервирующего средства. Отметили, что сосуществование совпадающих результатов видно было в 61,4%. Процент несовпадающих 38,6% реакции охватывал 15,2% мнимо отрицательных результатов и 23,4% заниженных результатов. Отметили, что молоко с добавкой глицерино-борного консерванта не пригодно к оценке в пробе Уайтсайда. Наличие консервирующего средства может вести к ошибочной оценке нормальности молока, неправильной интерпретации состояния здоровья молочных желез коров и осложнять борьбу с маститом.
EN
Employing Whiteside’s test 381 samples of milk containing conservative additions (glycerol and boric acid) or not (control) have been tested. Consistent results were obtained only in 61.4%. Inconsistent findings comprised 15.2% of false negative and 23.4% of lowered results. It was found that milk with the conservative preparations should not be used in Whiteside’s test. The presence of those substances might lead to false assessment of milk, inadequate evaluation of the state of udders, and therefore to hamper to combat mastitis.
RU
Цель работы состояла в определении в условиях in vitro чувствительности к антибиотикам штаммов Staph. aureus, изолированных из клинических форм мастита, и оценка возможного влияния биостимуляции вымени на антибиотикорстойчивость изолированных микроорганизмов. Материал для исследований составляли 542 штамма золотистого стафилококка, изолированных из воспалительного секрета вымени. Исследования показали, что в группе коров, позвергнутых биостимуляции, последовал резкий рост чувствительности к пенициллину. Разница в частоте появления пенициллиночувствительных стафилококков между исследуемой и контрольной группами составила 60% в 1986 г. Низкий процент штаммов, чувствительных к окситетрациклины и неомицину. в подопытной гpуппе мог быть вызван применением препарата Mastalone в боpьбе с маститом. Полученные результаты показывают, что применение биостимуляции вымени, а тем самым ограничение ввода антибиотиков, может уменьшить число устойчивых штаммов.
EN
The purpose of the work was to determine the sensitivity of the S. aureus strains isolated from subclinical mastitis to antibiotics and to evaluate the influence of udder biostimulation on the sensitivity of isolated strains to antibiotics. The studies were carried out on 542 strains of S. aureus isolated from the inflammatory secretions of udders. It was found that in the group of cows in which biostimulation was performed the strains were more often sensitive to penicillin than those of the control group; the difference of frequency was 60%. A low percentage of strains sensitive to oxytetracycline and neomycin in the experimental group might be caused by the drug Mastalone used in the control of mastitis.
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tom 46
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nr 11
EN
The influence of residues in milk at the experimental concentrations detected by microbiological method of some sanitizing substances on the result of the Penzym test was evaluated. The following washing substances: Ixi, Michasia, Krasula and Mirax at concentrations 0.1—1.0% and disin­fectants: lime, Mastycyd, Incozan W, Chloramin T, chlori­nated lime, lime water at concentrations 0.2—1.0%, H202 at concentrations 0.025—1.0% and Chlorogen D at concentra­tions 0.08—0.5% and 1.0% were examined. These substances were used in the test separately or in an association with penicillin. It was found that Chloramin T, Ixi, Chlorogen D, Mastycyd, H202 and chlorinated lime afected the colour change in the Penzym test and hence these substances might be detected in the test. A false positive results are obtained when milk acidity is changes from pH 6.1. The residues of experimental concentrations i.e. 0.5% of Chlorogen D, 0.7% Chloramin T, 0.3% of chlorinated lime eliminated a possibility to detect 0.017 iu of penicillin/cm3. The microbiological test was free from this unsuitability. These preliminary experiments were done under laboratory conditions.
EN
Pathozone for treatment purposes was administered in two groups of cows with mastitis. The first group consisted of 37 cows suffered from the acute form of the disease and it concerned 74 udder lobuses, the second concerned 27 cows with the signs of the subacute mastitis comprising 28 udder lobuses. There were assayed the kind of secretion, general and local signs at day 0 and 2-4-7 days and 14 days after the treatment. The number of skin lesions and mechanical injuries round the teat canal orifices influenced the development of acute mastitis (at 0,05 < p < 0,10). In the both groups general and organ signs of the disease disap­peared though the frequency of the signs at the beginning was different — 59,6% and 2,7% at day 7, and 0% at day 14. The efficacy of Pathozone was well marked in the first 4 days of treatment and at end the effect was similar independently upon the clinical from of the disease. The degree of clinical recoveries was 89,2% in the group of 37 cows and 100% in the second group; in the both groups there was 93,73% of resoveries.
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tom 46
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nr 10
EN
There were compared microbiological indices of 20 milk raw materials taken at random from the large scale cowsheds and 20 samples from the stations of individual deliverers. The microorganisms were found in the amount of 32.5X10® and 17.5X10®/1 ml respectively. The titer of E. coli was 10—4 (40%h) or 10—2 (30%), enterococi 10~3 (75%), 10-3 (50%), and staphylococci in 90% and 65% of the samples. The higher number of acidophylic, caseolytic and psychrotrophic microflora was noted in the raw materials of individual deliverers. The results of microbiological examinations were not in agreement with the healthy state of cows from the large state farms. The control of mastitis showed that: a) 74.5% cows was free from udder diseases, b) 8.55% suffered from subclinical diseases and 14.5% from aseptic forms of mastitis. The yield of milk was increasing every year and it was 30531 in 1983 and 37691 in 1988. The results indicate that a lack of market mechanisms in respect to milk production does not influence profitably the effects of the control of mastitis in cows. The healthy state of udders in cows of individual farms expressed by positive Whiteside’s test was 85% and in the comparable group — 40%.
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