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Design of experiment approach to optimize the high resolution ICP-OES method for biomonitoring Zn level in human blood samples

100%

Sawicki J. , Wojciak M. , Dresler S. , Torbicz A. , Skalska-Kaminska A. , Sowa I.

tom 28

nr 4

Lamium album extracts express free radical scavenging and cytotoxic activities

100%

Paduch R. , Matysik G. , Wojcik-Kosior M. , Kandefer-Szerszen M. , Skalska-Kaminska A. , Nowak-Kryska M. , Niedziela P.

nr 4

569-580

Lamium album, commonly known as “white dead nettle,” is a perennial herb widely used in folk medicine. The present paper presents the toxic, anti-proliferative, and free radical (DPPH˙) scavenging activities of methanol and ethyl acetate extracts of that plant. In order to determine the biologically active compounds, the plant extracts were separated by high performance thin layer chromatography (HPTLC) on silica gel Si 60 F254 and high performance liquid chromatography (HPLC) combined with densitometry. Methanol extract was rich mainly with flavonoids and phenolic acids. Ethyl acetate extract contained mainly triterpenes. Both extracts showed no toxic effects against normal human skin fibroblasts (HSF) in the range of applied concentrations (25–225 μg/ml). Anti-proliferative activity revealed that methanol extract expressed lower inhibitory properties than ethyl acetate one. The MTT test was, however, less sensitive than Neutral Red (NR) assay. Ethyl acetate extract did not exhibit DPPH˙ radical scavenging activity. Methanol extract reduced the radical of about 29% at the highest applied concentration (225 μg/ml). Both extracts slightly influenced cellular cytoskeleton organization and amount, and size of agyrophilic nucleolar organizer regions (AgNOR) protein deposits. These findings suggest that extracts of Lamium album exhibit potential usefulness in preparation of new natural formulations.