Wyniki wyszukiwania - Biblioteka Nauki

1

Human herpesvirus type 8 seroprevalence among patients in immunosuppression state

100%

Trzcinska A. , Litwinska B.

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tom 50

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nr 3-4

301-303

2

Wplyw komorek hodowli Namalwa na replikacje HSV-1

100%

Wieczorek M. , Litwinska B.

Medycyna Doświadczalna i Mikrobiologia

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2009

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tom 61

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nr 2

197-202

3

Differences in inhibition of apoptosis depending on the virulence of used herpes simplex virus type 1 strains. Function of interferon alpha in apoptotic death of virus infected cells

100%

Rechnio M. , Litwinska B.

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tom 52

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nr 3

4

Association of EBV infection with lymphomas

100%

Trzcinska A. , Litwinska B.

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nr 2

5

Porównanie aktywności wirusostatycznej kwasów: kawowego, chlorogenowego i rozmarynowego

80%

Borkowski B. , Biesiadecka A. , Litwinska B.

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tom 42

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nr 4

317-321

PL

Badania aktywności wirusostatycznej wykazały, że miano wirusa herpes simplex (HSV-1) w układzie z kwasem kawowym i rozmarynowym było niższe o około 3 lg w porównaniu do miana wirusa w układzie kontrolnym. W przypadku kwasu chlorogenowego takiego działania nie obserwowano.

EN

It has been shown that caffeic and rosmarinic acids reveals activity against herpes simplex virus (HSV-1). Chlorogenic acid had no virusostatic activity.

6

Metoda shell vial w diagnostyce zakazen wywolanych wirusem cytomegalii

80%

Siennicka J. , Litwinska B. , Kantoch M.

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nr 3-4

293-299

PL

Do diagnostyki zakażeń wirusem cytomegalii wykorzystywanych jest wiele metod laboratoryjnych o różnej wartości diagnostycznej. Jednak stwierdzenie w badanym materiale obecności zdolnego do replikacji wirusa stanowi niepodważalny dowód aktywnego zakażenia.

EN

The paper presents description of „shell vial" method and results obtained in the study of 331 materials derived from 3 different groups of patients. Problems of referring materials to laboratory, choice of type of material and method of staining are discussed. Also advantages (detection of virus capable of replication) and disadvantages (low sensitivity, toxicity of materials for detector cells) are discussed.

7

Wirusy papilloma i herpes oraz wybrane czynniki ryzyka w etiopatogenezie raka szyjki macicy. I. Zastosowanie hybrydyzacji kwasow nukleinowych do wykrywania zakazen HPV

80%

Szkoda M. T. , Rekosz M. , Litwinska B. , Kantoch M.

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tom 51

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nr 3-4

383-392

8

Isolation of Sabin-like polioviruses from sewage in Poland

80%

Figas A. , Wieczorek M. , Zuk-Wasek A. , Litwinska B.

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tom 67

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nr 1

EN

As a complement to the active search for cases of acute flaccid paralysis, environmental sampling was conducted from January to December 2011, to test for any putative polio revertants and recombinants in sewage. A total of 165 environmental samples were obtained and analyzed for the presence of polioviruses by use of cell culture (L20B, RD and Caco-2) followed by neutralization and reverse-transcription polymerase chain reaction. Out of the 31 CPE positive samples, 26 contained one and 5 two different serotypes, yielding a total of 36 PVs. The microneutralization test revealed the presence of 7, 10 and 19 strains belonging to poliovirus serotype 1, 2 and 3, respectively. The genomic variability of 36 poliovirus strains was examined by the restriction fragment length polymorphism assay (RFLP). By combined analyses of two distant, polymorphic segments of the viral genome, one situated in the capsid protein VP1 coding region and the other in the 3D-polymerase coding region, we screened for the putative poliovirus revertants and recombinants. All detected PVs were classified as vaccine strains on the basis of RFLP-VP1 test. None of wild-type PVs or vaccine derived polioviruses were detected. RFLP assay also revealed the presence of 11 recombinants in 3D-polymerase coding region. Nine isolates appeared to be S3/S2, one S3/S1 and S1/S2 recombinant in analyzed 3Dpol region. This study revealed, through environmental monitoring, the introduction of SL PVs into the population associated with the routine use of OPV in Poland before the April 2016. Our findings demonstrate the usefulness of environmental surveillance in the overall polio eradication program.

9

Profile of antibodies against Epstein-Barr virus in patients with blood disorders

80%

Zuk-Wasek A. , Litwinska B. , Traczyk Z.

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2008

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tom 52

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nr 4

EN

The aim of the study was to investigate the profile of Epstein-Barr virus (EBV) antibodies and to compare this profile with the detectability of EBV DNA and compare this profile in healthy people with the profile in patients with blood disorders. The profile of antibodies was examined in serum from the following groups: 40 patients with blood disorders, but without EBV DNA in their lymphocytes, 44 patients with blood disorders and EBV DNA in lymphocytes, and 46 healthy people. EBV antibodies were detected by using ELISA commercial kits. The obtained results were analysed by Fisher's exact test. No significant differences in antibody profiles were found between EBV DNA-positive and EBV DNA-negative sera. Between patients and the control group, considerable differences were observed in reactivation and in past infection, and a significant difference was observed in chronic infection. These analyses suggest that EBV reactivation or chronic infection can be related with blood disorders and have an influence on the course of blood disorders.

10

Niepandemiczne koronawirusy człowieka – charakterystyka i diagnostyka

80%

Abramczuk E. , Pancer K. , Gut W. , Litwinska B.

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tom 56

|

nr 2

11

Wplyw wysokich dawek gammablobuliny [IVIG] na przebieg zakazenia HSV-1 u myszy poddanych immunosupresji

80%

Litwinska B. , Bucholc B. , Kantoch M.

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tom 44

|

nr 3-4

167-171

12

The utility of Caco-2 cells in isolation of enteroviruses from environmental and clinical material

80%

Wieczorek M. , Ciacka A. , Witek A. , Litwinska B.

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nr 1

EN

The work presented here demonstrates the utility of Caco-2 cells in the isolation of enteroviruses (EVs) from environmental and clinical materials. Thirty-two samples of cerebrospinal fluid positive in Pan-entero RT-PCR were taken for EV strain isolation in cell culture. Out of the 32 samples analysed, 22 (68.75%) were positive for enteroviruses by isolation in Caco-2 cells, and 10 (31.25%) were positive by isolation in RD cells. High viral titre in clinical specimens resulted in rate increase for isolation in Caco-2 cells and RD cells (87.5% and 50%, respectively). Also, the probability of isolation of enteroviruses from sewage in Caco-2 cells was 20 times higher that in RD cells. We proved that Caco-2 cells were more effective than RD cells in enterovirus isolation, irrespective of the material used in the inoculation process.

13

Przeciwwirusowa aktywność handlowych preparatów immunoglobulin

80%

Litwinska B. , Bucholc B. , Sadowski W. , Kantoch M.

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nr 1-2

14

Detection of EBV infection in different etiologic groups of patients

80%

Trzcinska A. , Litwinska B. , Kantoch M.

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tom 50

|

nr 3-4

275-280

15

Wplyw doboru szczepow wirusa cytomegalii na stymulacje cytokin panelu Th1-Th2

80%

Siennicka J. , Gut W. , Litwinska B.

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nr 3

309-316

16

Studies on the stability of HSV-1 temperature sensitive 28 degrees Celsius clone

80%

Litwinska B. , Biesiadecka A. , Kantoch M.

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nr 5-6

17

The detection of enteroviruses in sewage using Caco-2 cells

71%

Wieczorek M. , Kuryk L. , Witek A. , Diuwe A. , Litwinska B.

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nr 1

EN

The work presented here demonstrates the utility of Caco-2 cells to detect enteroviruses in sewage. Viruses were concentrated by beef extract elution and organic flocculation prior to analysis by cell culture assays and RT-PCR. Enteroviruses were detected in all sewage samples, but only one sample was positive solely in RT-PCR assay. We proved that Caco-2 cells were more effective than RD and L20B cells in enterovirus isolation, depending on procedures used in the inoculation process.

18

Sequence based typing and pre-absorption test in retrospective analysis of a pseudo-outbreak of Legionella infections differentiates true cases of legionellosis

61%

Pancer K. , Jahnz-Rozyk K. , Kucharczyk A. , Gut W. , Kucharczyk P. , Elverdal P. L. , Litwinska B.

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2012

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tom 19

|

nr 3

19

Przydatnosc testu cytotoksycznego do badania poziomu przeciwcial bloniczych w surowicach uodparnianych zwierzat

61%

Kudelski Z. , Wysokinska T. , Janaszek W. , Zakrzewska A. , Litwinska B. , Biesiadecka A.

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tom 48

|

nr 1-2

95-98

20

Ocena przydatnosci roznych wariantow metody PCR oraz metody hybrydyzacji kwasow nukleinowych w wykrywaniu zakazenia CMV u pacjentow z immunosupresja

61%

Siennicka J. , Trzcinska A. , Litwinska B. , Durlik M. , Seferynska I. , Palynyczko G. , Kantoch M.

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tom 52

|

nr 3

283-293

PL

Porównano diagnostyczną przydatność metody hybrydyzacji i różnych wariantów PCR do wykrywania DNA wirusa cytomegalii w próbkach krwi pacjentów z immunosupresją.

EN

In diagnosis of CMV infection various laboratory methods are used. The methods based on detection of viral nucleic acids have been introduced routinely in many laboratories. The aim of this study was to compare nucleic acid hybridisation method and various variants of PCR methods with respect to their ability to detect CMV DNA. The studied material comprised 60 blood samples from 19 patients including 13 renal transplant recipients and 6 with acute leukaemia. The samples were subjected to hybridisation (Murex Hybrid Capture System CMV DNA) and PCR carried out in 3 variants: with one pair of primers (single PCR), nested PCR and Digene SHARP System with detection of PCR product using a genetic probe in ELISA system. The sensitivity of the variants ranged from 10° particles of viral DNA in nested PCR to 102 in single PCR. The producer claimed the sensitivity of the hybridisation test to be 3 x 105 and it seems to be sufficient for detection of CMV infection. The obtained results show that sensitivity of hybridisation was comparable to that of single PCR and the possibility of obtaining quantitative results makes it superior, on efficacy of antiviral therapy. especially in monitoring CMV infection in immunossupressed patients and in following the efficacy of antiviral treatment.