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Chlamydia pneumoniae - biologia i chorobotworczosc

100%

Podsiadly E. , Tylewska-Wierzbanowska S.

nr 2

145-166

Wystepowanie Bartonella spp. w wybranych rezerwuarach i wektorach na terenie Polski

100%

Podsiadly E. , Tylewska-Wierzbanowska S.

nr 3

275-281

Czy Chlamydophyla pneumoniae moze byc czynnikiem etiologicznym chorob nieinfekcyjnych?

100%

Podsiadly E. , Tylewska-Wierzbanowska S.

nr 2

127-136

Obecność laktoferyny w kale jako wskaźnik zakażenia Clostridium difficile u dzieci

80%

Dabrowska S. , Demkow U. , Podsiadly E.

nr 1

Presence of Bartonella spp. in various human populations

80%

Chmielewski T. , Podsiadly E. , Tylewska-Wierzbanowska S.

nr 1

Bartonella spp. bacteria are significant human pathogens and the agents of bacterial zoonosis acquired from an animal companion. The aim of the study was to determine the seroprevalence of two of the most common Bartonella species B. henselae and B. quintana in various human populations. The studied groups included: alcoholics, intravenous drug users, veterinarians, cats' owners. Blood samples were collected and cultured on chocolate agar plates and in mouse fibroblasts L-929 cell line culture. The levels of Bartonella henselae IgM and IgG antibodies were determined by indirect immunofluorescence assay. Specific B. henselae IgG were detected in 48.3% of homeless alcoholics, in 45.0% veterinarians and in 53.3% cats' owners. The differences in the prevalence of B. henselae antibodies between the studied groups and a control group were statistically supported. No homeless intravenous drug users had specific B. henselae and B. quintana antibodies. High titers of B. quintana IgG antibodies were detected in two homeless alcoholics. Bartonella spp. was cultured on chocolate blood agar plates from blood samples from 2 alcoholics. The isolates were identified as B. henselae by PCR amplification of the riboflavin synthase gene (ribC). The results prove that B. henselae and B. quintana, emerging human pathogens, are present and widely distributed in Poland in such specific risk groups as: alcoholics, veterinarians and cats' owners.

Molecular epidemiology of Q fever in Poland

61%

Chmielewski T. , Sidi-Boumedine K. , Duquesne V. , Podsiadly E. , Thiery R. , Tylewska-Wierzbanowska S.

nr 1

9-13

Coxiella burnetii is the etiologie agent of Q fever, a worldwide distributed zoonosis, accountable for serious health problem both for humans and animals. The exposure to C. burnetii infected animals and their products is the main risk factor for Q fever in humans. Several outbreaks of Q fever have been described in Poland which sources were recognized to be related to imported animals and their products or to wildlife using serological methods. Moreover, some of them have been confinned by isolation of C. burnetii strains. In this study, multispacer sequence typing (MST) and multiple loci variable number tandem repeats (VNTR) analysis (MLVA) have been used to characterize C. burnetii strains isolated in Poland. A total of two sequence types (MST) and four MLVA types were identified among 6 C. burnetii isolates examined. This study highlighted the usefulness of these methods in the improvement of epidemiological investigations of Q fever loci on the Polish territory.