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2 Journal of Applied Genetics

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Tools of the trade: diagnostics and research in domestic animal cytogenetics

100%

Iannuzzi L. , Berardino_Di D.

Journal of Applied Genetics

2008

tom 49

nr 4

357-366

This paper describes the most common cytogenetic techniques we routinely adopt in our laboratories for producing high-resolution banding on prometaphase stage chromosomes, from synchronized or nonsynchronized blood cultures. Special emphasis is given to the FISH procedures applied to prometaphase chromosomes for mapping purposes. Each section includes historical information, basic principles for the given technique, its primary use in veterinary cytogenetics, and major limitations. Supplementary material (protocols and chemicals used) are available on our website. Even though these techniques mainly refer to the Bovidae, they can be easily extended and adapted to members of other taxa.

Chromosomal assignment of R-spondin genes in the donkey (Equus asinus, 2n = 62)

61%

De_ L. L. , Genualdo V. , Perucatti A. , Pia_ D. M. G. , Molteni L. , Iannuzzi L. , Parma P.

Journal of Applied Genetics

2010

tom 51

nr 3

319-321

R-spondins constitute a recently discovered small family of growth factors, and the evidence of their role in several developmental pathways is growing fast. In this work we describe the chromosomal location of the four RSPO genes in the donkey. Using horse BACs, we localized RSPO1 on EAS 5q23, RSPO2 on EAS 12q13, RSPO3 on EAS 24q26, and RSPO4 on EAS 15p13. Moreover, RSPO2, RSPO3, and RSPO4 are the first genes mapped on donkey chromosomes 12, 24, and 15, respectively.