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The viability of the genetically diverse C. jejuni and C. coli strains in the macrophage J774 cell line

100%

Krutkiewicz A. , Klimuszko D.

nr 1

The intracellular survival of Campylobacter has been described within epithelial cells as well as in macrophages in vitro. The goal of this study was to estimate the viability of the genetically diverse C. jejuni and C. coli strains in the macrophage J774 cell line. Strains selected for analysis differed with regard to the occurrence of genes encoding specific virulence factors. The present work indicates that was no correlation between the source of isolates and relative intracellular survival.

Strategies of constructing recombinant vaccines

100%

Salamaszynska-Guz A. , Klimuszko D.

nr 1

Vaccines are the most effective prophylactic tool in veterinary medicine. Despite the great success of many vaccines used currently, there is still a constant need for their improvement. An ideal vaccine should contain a variety of immunogens, be safe and efficacious and induce broad humoral and cell-mediated immunity with one or, at most, two administrations given orally rather than by injection, and should be inexpensive. Traditional approaches include attenuated live vaccines, inactivated vaccines and subunit vaccines. Recently, scientific advances in molecular biology, immunology and bioinformatics, as well as the growing number of sequenced genomes of pathogens, have led to significant progress in respect to understanding virulence mechanisms at the molecular level. Genetic engineering has been applied to obtain recombinant bacterial and viral genomes in order to produce a modified and safe product useful in vaccine development. This article presents the progress and novel strategies used in creating new generation vaccines. It focuses on methods of searching for vaccine candidates to construction of vaccines based on recombinant DNA or proteins.

Influence of mutation in cj0183 and cj0588 genes for colonization abilities of Campylobacter jejuni in Caco-2 cells using confocal laser scanning microscope

80%

Salamaszynska-Guz A. , Godlewski M. M. , Klimuszko D.

nr 2

The cj0183 and cj0588 genes identified in the Campylobacter jejuni NCTC 11168 genome encode proteins with homology to virulence factors found in other bacteria. Previous studies showed that single mutation in the cj0183 gene does not affect adhesion of C. jejuni to the Caco-2 cell line whereas protein encoded by cj0588 is involved in adherence to the Caco-2 cells. In the presented study differences in invasion index were observed between mutants in both genes and single mutation of cj0588 in 81116 and 81-176 C. jejuni strains. This fact indicates that Cj0183 protein might play some role in invasion of bacteria into host cells.

Influence of mutation in cj0183 and cj0588 genes for colonization abilities of Campylobacter jejuni in Caco-2 cells using confocal laser scanning microscope

80%

Sałamaszyńska-Guz A. , Godlewski M. M. , Klimuszko D.

Production of enterotoxins by strains of Escherichia coli isolated from pigs

61%

Niemialtowski M. , Szynkiewicz Z. , Binek M. , Klimuszko D. , Ciosek D. , Jakubowski T.

nr 3-4

The viability of the genetically diverse C. jejuni and C. coli strains in the macrophage J774 cell line

60%

Krutkiewicz A. , Klimuszko D.