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Phenotypic and genotypic characterisation of enterococci isolated from milk of dairy cows with mastitis

100%

Zastempowska E.

Postępy Mikrobiologii. Suplement

2017

tom 56

nr 1

Mycotoxin – induced apoptosis in swine kidney epithelial cells

51%

Chrustek A. , Twaruzek M. , Zastempowska E. , Grajewski J.

Annales Universitatis Paedagogicae Cracoviensis Studia Naturae

2016

tom 01

Virulence factors in coagulase-negative staphylococci isolated from cows with subclinical mastitis

51%

Malinowski E. , Klossowska A. , Zastempowska E.

Bulletin of the Veterinary Institute in Puławy

2011

tom 55

nr 4

The aim of this study was to investigate the presence of virulence genes in 46 coagulase-negative staphylococci (CNS) strains isolated from 46 udder quarters of 46 cows with subclinical mastitis. The isolates were examined phenotypically (cultural features, Gram stain, catalase, coagulase, API tests) and genotypically for: icaA, icaAB, icaD, atlE, bhp, agrA, sarA, mecA, and blaZ genes. These isolates had no nuc and coa genes, and were identified as S. warneri (11 strains), S. chromogenes (10), S. xylosus (seven), S. epidermidis (one), S. hyicus (one), and other Staphylococcus species (16). The single virulence genes or their combinations were detected in nine (19.6 %) of isolates. The following genes were found: blaZ (in four isolates), mecA (3), sarA (3), agrA (3), and atlE (three). None of the strains harboured the bhp, icaA, icaD, and icaAB genes. In conclusion, subclinical mastitis in cows can be caused by different species of CNS that harbour different virulence factors.

Występowanie metycylinooporności gronkowców koagulazo-ujemnych izolowanych z mastitis u krów

51%

Lassa H. , Skorzewski R. , Zastempowska E. , Smulski S.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

2012

tom 07

nr 2 Monografia

Etiological agents of bovine mastitis in Poland

45%

Krukowski H. , Lassa H. , Zastempowska E. , Smulski S. , Bis-Wencel H.

Medycyna Weterynaryjna

2020

tom 76

nr 04

This research paper addresses the hypothesis that the incidence of various bovine mastitis pathogens is subject to change over time and across regions. The aim of the research was to determine the pathogen species that are currently the etiological agents of mastitis in Polish dairy herds. This comprehensive study was conducted in the period 2009-2017 and included all the Polish provinces, distinguishing between those with high and low numbers of dairy cattle. Over eight years 38,852 quarter milk samples were obtained from 22,587 cows with clinical and subclinical mastitis from herds located in all 16 provinces in Poland. Milk samples were cultured using standard microbiological methods. Most of them were positive for microorganisms but there was also part of bacteriologically negative and contaminated samples. Gram-positive catalase-negative cocci and staphylococci were the most prevalent pathogens. The growth of other microorganisms such as Gram-negative and Gram-positive bacilli, yeasts and algae was observed at lower frequencies. There were differences in the prevalence of microorganisms depending on the region. In the eastern part of the country a higher number of Streptococcus agalactiae infections than in other regions was observed. This paper provides the latest data on microbial species, frequency of their occurrence and regional location within Poland. The knowledge of mastitis pathogens enables appropriate treatment and to make prevention recommendations for the herds.

Udział świni domowej w puli genowej naturalnych populacji dzika europejskiego w Polsce

45%

Dzialuk A. , Zastempowska E. , Skorzewski R. , Twaruzek M. , Grajewski J.

Postępy Mikrobiologii. Suplement

2017

tom 56

nr 2